OPTICAL AND MOLECULAR APPROACHES TO THE STUDY OF CHEMICAL AGENTS

研究化学试剂的光学和分子方法

• 批准号: 8362624
• 负责人: TATIANA B KRASIEVA
• 金额: $ 1.21万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362624
• 关键词: 2-chloroethyl ethyl sulfide; Adhesions; Biosensor; Biotechnology; Cell Cycle; Cell physiology; Cellular Structures; Chemical Agents; Cytoskeleton; Development; Event; Exposure to; Funding; Future; Goals; Grant; Knowledge; Lasers; Molecular; Mono-S; Mustard; Mustard Gas; National Center for Research Resources; Optics; Principal Investigator; Research; Research Infrastructure; Resources; Role; Source; Tissues; United States National Institutes of Health; Wound Healing; cost; effective therapy; treatment strategy

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The overall goal of this project is to apply modern optical and molecular/cellular probes to study SM effects on cell structure and function by using 2-chloroethyl ethyl sulfide, a mono-functional sulfur mustard (CEES). This research effort is intended to enhance our understanding of the primary cellular effects of SM exposure. To this end, this fundamental knowledge of the cellular and molecular events underlying SM exposure will serve as a keystone to future biosensor/probe development and will facilitate the development of more effective treatment strategies. The specific objectives of this project are: (1) to examine the effects of CEES (half-mustard) at the molecular and cellular level, specifically, on the cytoskeleton in relationship to the cell cycle, (2) to determine a potential role for Src in the disorganization of the cytoskeleton and adhesion following exposure to CEES, (3) to study the effects of CEES at the tissue level as related to wound healing.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该项目的总体目标是应用现代光学和分子/细胞探针来研究SM对细胞结构和功能的影响，并使用2-氯乙基硫化物（一种单功能性硫芥末（CEES））。这项研究工作旨在增强我们对SM暴露的主要细胞影响的理解。为此，对SM暴露的细胞和分子事件的这种基本知识将成为未来生物传感器/探针开发的基石，并将促进开发更有效的治疗策略。该项目的具体目标是：（1）检查分子和细胞水平上的CEE（半粘量）在与细胞周期之间关系的细胞骨架上的影响，（2）确定SRC在与C的细胞骨架和粘附之间的混乱中的潜在作用（3）与C hun cees相关的CEES（3）疗法的效果。