Flow Cytometry and Confocal Microscopy Shared Resource

流式细胞术和共焦显微镜共享资源

基本信息

批准号：
10625766
负责人：
Patrick Kellish
金额：
$ 7.83万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-06-01 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10625766
关键词：
Abbreviations Accounting Adherence Antibodies Applications Grants Back Biological Markers Biometry Biotechnology Brain COVID-19 pandemic Cancer Center Cancer Center Support Grant Cancer Control Cell Separation Cells Cellular Morphology Certification Charge Clinical Collaborations Communities Complex Confocal Microscopy Consultations Data Data Analyses Development Doctor of Philosophy Educational workshop Effectiveness Ensure Equipment Experimental Designs Feedback Florida Flow Cytometry Funding Future Genetic Research Growth Health Hour Image Immune Immune response Immunobiology Immunologist Immunophenotyping Immunotherapy Infrastructure Institution Knowledge Laboratories Lasers Last Name Letters Malignant Neoplasms Methods Microscopy Mission Modernization NCI Center for Cancer Research Peer Review Phenotype Policies Population Sciences Postdoctoral Fellow Procedures Productivity Publications RNA vaccine Recommendation Reproducibility Research Research Activity Research Project Grants Research Support Reservations Resource Sharing Resources Sampling Scanning Science Services Solid Neoplasm Sorting Strategic Planning Students Surface Surface Antigens Surveys System Technology Tissues Training United States National Institutes of Health Universities Vaccine Clinical Trial Vendor anticancer research cost cost effective design exosome experience first-in-human fluorescence microscope improved instrument instrumentation live cell imaging member multi-photon operation particle programs recruit response satisfaction single cell analysis student training time use treatment response tumorigenesis ultra high resolution

项目摘要

FLOW CYTOMETRY AND CONFOCAL MICROSCOPY SHARED RESOURCE (CYT-SR): ABSTRACT CYT-SR provides University of Florida Health Cancer Center (UFHCC) members with cost-effective, state-of- the-art flow cytometry and modern confocal microscopy expertise, instrumentation, and services to support their research activities. The CYT-SR is an institutionally-managed shared resource (SR) that is supported by UFHCC through a partnership with the UF Interdisciplinary Center for Biotechnology Research (ICBR). It is co-located within the Cancer & Genetics Research Complex and McKnight Brain Institute, providing convenient access for UFHCC members. The CYT-SR has 4 staff with over 22 years combined experience including Kellish, the Scientific Director, an immunologist with more than 7 years of experience in all aspects of flow analysis. Key technologies available in the CYT-SR include multi-parameter flow cytometry, cell sorting, live cell imaging via confocal microscopy, surface biomarker-based cancer and immune cell phenotyping, assessment of tissue and cell morphology by confocal microscopy, and small particle and exosome analysis. During the recent COVID-19 pandemic, the CYT-SR was identified as an essential campus resource and remained open and accessible to all users. Instrument reservations, equipment usage time, and technician-assisted activities are tracked by the UFHCC and charged back via the CrossLab laboratory management system. Since 2016, the CYT-SR was utilized by 78 unique UFHCC users (43 with peer-reviewed funding) accounting for ~24% of all users of the facility. CYT-SR use by UFHCC members increased 5.3-fold from 900 hours in 2016 to 4,800 hours in 2021. The CYT-SR supported more than 180 publications, with 24% having IF >10. Since the 2019 CCSG submission, 484 students, postdoctoral fellows, and technical staff from UFHCC member laboratories were trained on CYT- SR instruments. Mechanisms of Oncogenesis and the Cancer Therapeutics and Host Response members represent the majority of UFHCC users. The effectiveness of the CYT-SR in enhancing member research productivity is assessed by annual surveys, feedback from the CYT-SR Scientific Advisory Group, and recommendations from the SR Oversight Committee. Due to the rising demand for CYT-SR resources by members, the UFHCC supported the purchase of a new Sony SP6800 Spectral Analyzer (2018), Cytoflex LX21 (2020), and a Becton Dickinson SYMPHONY A3 (2020). To support first-in-human RNA vaccine clinical trials, UFHCC supported the recruitment of a PhD-level immunologist to the CYT-SR to perform flow-based immunophenotyping. Future growth of the CYT-SR are in alignment with the UFHCC strategic plan, Momentum 2027, and user satisfaction surveys, and include upgrades such as an acquisition of a BD FACSYMPHONY S6 cell sorter, increasing capabilities and services in super high-resolution microscopy and single cell analysis, and in collaboration with BQS-SR, expansion of advanced training in the application of biostatistics to cytometric analysis.

流式细胞仪和共聚焦显微镜共享资源（CYT-SR）：摘要 Cyt-SR为佛罗里达大学健康癌症中心（UFHCC）成员提供成本效益，最新 ART流式细胞术和现代共聚焦显微镜专业知识，仪器和服务以支持其研究活动。 CYT-SR是由UFHCC支持的机构管理的共享资源（SR）通过与UF跨学科生物技术研究中心（ICBR）的合作关系。它是共同的在癌症与遗传学研究院和麦克奈特脑研究所中，为方便的访问提供了 UFHCC成员。 Cyt-SR拥有4位员工，拥有超过22年的合并经验，包括Kellish，科学总监，一名免疫学家，在流动分析的各个方面拥有超过7年的经验。钥匙 Cyt-SR中可用的技术包括多参数流式细胞术，细胞分类，通过活细胞成像通过共聚焦显微镜，基于表面生物标志物的癌症和免疫细胞表型，组织和共聚焦显微镜以及小颗粒和外泌体分析的细胞形态。在最近的Covid-19期间大流行，Cyt-SR被确定为必不可少的校园资源，并保持开放和访问所有用户。仪器预订，设备使用时间和技术人员辅助活动由 UFHCC并通过CrossLab实验室管理系统充电。自2016年以来，Cyt-SR是由78个唯一的UFHCC用户（43个具有同行评审的资金）使用，占所有用户的24％设施。 UFHCC成员使用的CYT-SR使用率从2016年的900小时增加到5.3倍，增加到2021年的4,800小时。 Cyt-SR支持了180多个出版物，其中24％的IF> 10。自2019年CCSG提交以来， 484名学生，博士后研究员和来自UFHCC成员实验室的技术人员接受过培训。 SR仪器。肿瘤发生和癌症治疗和宿主反应成员的机制代表大多数UFHCC用户。 CYT-SR在增强成员研究方面的有效性生产率通过年度调查，CYT-SR科学咨询小组的反馈以及 SR监督委员会的建议。由于对Cyt-SR资源的需求不断增加成员，UFHCC支持购买新的Sony SP6800 Spectral Analyzer（2018），CytoFlex LX21 （2020）和Becton Dickinson Symphony A3（2020）。为了支持首次人类RNA疫苗临床试验， UFHCC支持将PHD级免疫学家招募到CYT-SR，以执行基于流量的免疫表型。 Cyt-SR的未来增长与UFHCC战略计划保持一致 2027年和用户满意度调查，并包括升级，例如收购BD FACSYMPHONY S6 细胞分类器，超高分辨率显微镜和单细胞分析中的功能和服务的增加，以及与BQS-SR合作，在应用生物统计学中扩展高级培训分析。