Spinning Disk Confocal and Single Molecule Localization Microscope

转盘共焦和单分子定位显微镜

• 批准号: 10177496
• 负责人: Jeffrey L Caplan
• 金额: $ 56.17万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2022-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177496
• 关键词: 3-Dimensional; Address; Anabolism; Biogenesis; Biomedical Research; Biotechnology; Cell Wall; Cells; Collagen Fibril; Communities; Confocal Microscopy; Contract Services; Crystalline Lens; Cytoskeleton; Delaware; Ensure; Erythrocytes; Experimental Designs; Fluorescence Microscopy; Funding; Grant; Human Resources; Image; Imaging Techniques; Institutes; Laser Scanning Confocal Microscopy; Light Microscope; Liquid substance; Lung; Measures; Methods; Microscope; Microscopy; Minor; Musculoskeletal Diseases; Organelles; Photobleaching; Phototoxicity; Preparation; Process; Recording of previous events; Research; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Signal Transduction; Speed; Technology; Tissue Engineering; Uncertainty; United States National Institutes of Health; Universities; bioimaging; bone; data acquisition; design; experience; extracellular vesicles; flexibility; instrument; instrumentation; interest; live cell microscopy; mechanotransduction; meetings; member; multimodality; pathogenic bacteria; programs; single molecule; tissue regeneration; tissue repair

Project Summary / Abstract: 13 NIH-funded investigators are seeking support under the Shared Instrumentation Grant Program to obtain an Andor Dragonfly spinning disk confocal microscope with single molecule localization capability. This multimodal microscope will bring unprecedented speed, resolution, sensitivity and large field of view to the users of the Bio- Imaging Center at the University of Delaware (UD). Spinning disk confocal microscopy is today’s state-of-the-art method for live-cell microscopy due to its reduced photobleaching and phototoxicity, compared to point-scanning confocal microscopy. Furthermore, 3D single molecule localization microscopy is a cutting edge super-resolution imaging technique with that has been attracting widespread interest in biomedical research. Neither of these technologies are currently available to the research community in Delaware, and the Dragonfly is thus set to fill two needed gaps at once. The current application targets key research projects from 10 major users and 8 minor users who have a critical need for the requested microscope. Thanks to its flexibility & multimodality, there is no doubt that this instrument will keep meeting the needs of a wide range of research projects throughout its lifetime. The Dragonfly will impact a broad range of research fields from our major users. This includes Dr. Caplan, who will address fundamental questions on how cellular organelles move and release signals; Drs. Elliott and Wang, who will examine collagen fibrils and bone mechanosensing in respect to musculoskeletal disorders; Dr. Fowler who will study the cytoskeleton in red blood cells and ocular lenses. Other major users include Dr. Gleghorn, who will measure dynamic processes in the developing lung; Dr. Jia, who examine engineered tissue substitutes for tissue repair and regeneration; Drs. Grimes and Neunuebel will study bacterial cell wall biosynthesis and pathogenic bacteria survival and replication in host cells, respectively; Dr. Vlachos will quantify fluid dynamics, and Dr. Tanis will seek to determine the mechanisms underlying the biogenesis of extracellular vesicles. The Dragonfly will be placed in a new state-of-the-art research building, in which imaging suites were specifically designed for high-end light microscopes. It will be managed by the Delaware Biotechnology Institute (DBI)’s Bio- Imaging Center, which is a research hub that serves the imaging needs of over 150 research groups at UD and beyond. DBI and UD have a long established history of providing very strong institutional support, and have committed space, personnel, service contract, and administrative support. A 5-year financial plan has been provided to ensure long-term sustainability. The Bio-Imaging Center has a strong track record for providing excellent and sustained user support and resource management. Four staff members, who have over 75 years of combined experience in fluorescence microscopy, will provide assistance to the Dragonfly’s users in experimental design, sample preparation, data acquisition, and analysis.

项目摘要 /摘要： 13 NIH资助的调查人员正在根据共享工具赠款计划寻求支持，以获得 具有单分子定位能力的Andor Dragonfly旋转磁盘共聚焦显微镜。这个多模式 显微镜将为生物的使用者带来前所未有的速度，分辨率，灵敏度和较大的视野 特拉华大学（UD）的成像中心。旋转磁盘共聚焦显微镜是当今的最先进的 与点扫描相比 共聚焦显微镜。此外，3D单分子定位显微镜是尖端超分辨率 在生物医学研究中，这种成像技术吸引了宽度有趣。这些都不是 技术目前可用于特拉华州的研究社区，因此蜻蜓将填补 同时需要两个差距。当前的应用程序针对10个主要用户和8个未成年人的关键研究项目 对所需显微镜急需的用户。感谢它的灵活性和多模式，没有 怀疑该工具将在其一生中继续满足广泛的研究项目的需求。 蜻蜓将影响主要用户的广泛研究领域。这包括卡普兰博士，他 将解决有关细胞细胞器如何移动和释放信号的基本问题；博士。埃利奥特和王， 谁将在肌肉骨骼疾病方面检查胶原蛋白原纤维和骨骼机械感应；福勒博士 谁将研究红细胞和眼镜镜片中的细胞骨架。其他主要用户包括Gleghorn博士， 谁将测量发育中的肺部动态过程；贾博士，检查了工程组织替代品 用于组织修复和再生；博士。 Grimes和Neuuebel将研究细菌细胞壁生物合成和 宿主细胞中的致病细菌存活和复制； Vlachos博士将量化流体动力学， Tanis博士将寻求确定细胞外蔬菜生物发生的基础机制。 蜻蜓将被放置在新的最先进的研究大楼中，其中成像套件是专门的 专为高端光显微镜设计。它将由特拉华州生物技术研究所（DBI）的生物管理 成像中心，这是一个研究中心，可满足UD和UD和150多个研究组的成像需求 超过。 DBI和UD在提供非常强大的机构支持的历史上有很长的历史，并且 承诺的空间，人员，服务合同和行政支持。一项为期5年的财务计划已经 提供以确保长期可持续性。生物成像中心有很强的往绩记录 优秀和持续的用户支持和资源管理。四名员工，他们有75年以上 荧光显微镜的综合体验将为蜻蜓的用户提供帮助 实验设计，样品制备，数据采集和分析。