Zeiss LSM710 Inverted Confocal Microscope

蔡司 LSM710 倒置共焦显微镜

• 批准号: 8826476
• 负责人: Jeffrey L Caplan
• 金额: $ 44.4万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826476
• 关键词: 15 year old; Apoptosis; Arts; Biocompatible Materials; Biomechanics; Biomedical Engineering; Biomedical Research; Biotechnology; Cardiovascular system; Cells; Classification; Communities; Consult; Contract Services; Core Facility; Delaware; Development; Engineering; Ensure; Equipment; Eye Development; Funding; Generations; HIV; Housing; Image; Imaging Techniques; Institutes; Life; Manufacturer Name; Microscope; Microscopy; Osteogenesis; Process; Research; Research Activity; Research Personnel; Research Project Grants; Salivary Glands; Schedule; Science; System; Thrombosis; Tissues; Training; United States National Institutes of Health; Universities; Vascular Graft; bioimaging; college; instrument; meetings; research study; vocal cord; 15 year old; Apoptosis; Arts; Biocompatible Materials; Biomechanics; Biomedical Engineering; Biomedical Research; Biotechnology; Cardiovascular system; Cells; Classification; Communities; Consult; Contract Services; Core Facility; Delaware; Development; Engineering; Ensure; Equipment; Eye Development; Funding; Generations; HIV; Housing; Image; Imaging Techniques; Institutes; Life; Manufacturer Name; Microscope; Microscopy; Osteogenesis; Process; Research; Research Activity; Research Personnel; Research Project Grants; Salivary Glands; Schedule; Science; System; Thrombosis; Tissues; Training; United States National Institutes of Health; Universities; Vascular Graft; bioimaging; college; instrument; meetings; research study; vocal cord

 DESCRIPTION (provided by applicant): The University of Delaware (UD) has a very high research activity (Carnegie Classification) and much of this activity focuses on diverse fields of biomedical research. In particular the College of Arts and Sciences and the College of Engineering depend heavily on advanced imaging instruments for research in tissues, cells and biomaterials. UD has maximized utilization of these instruments with an established multi-user imaging core facility, which is responsible for maintaining, scheduling equipment usage, training users and consulting on advanced technical projects or development of new imaging techniques. One of the first confocal microscopes we acquired at UD, an LSM510 VIS confocal microscope, is housed in the Delaware Biotechnology Institute (DBI) Bio-Imaging Center at UD. The instrument is now 15 years old and no longer in use due to poor image quality and the manufacturer no longer providing service contracts as of September 2009. This application is a resubmission requesting funds to replace this obsolete instrument, which no longer meets the needs of our user community, with an LSM710 inverted confocal microscope with an environmental chamber. The proposed system will be located in the microscopy suite at the DBI and administered by the Bio- Imaging Center technical staff. This will ensure access to the proposed microscope to all investigators who utilize the DBI Bio-Imaging Center, which is a critical core facility for numerous NIH funded research projects at UD. Currently, the DBI Bio-Imaging Center only has LSM510 generation inverted confocal microscopes that lack the sensitivity and environmental control for live-cell experiments. Therefore, we are requested an LSM710 inverted confocal microscope to support NIH funded investigators researching a diverse array of biomedical processes that include engineering vascular grafts, programmed cell death, cardiovascular, biomechanics, salivary glands, vocal fold bioengineering, eye development, thrombosis, bone formation and HIV assembly.

 描述（由应用程序提供）：特拉华大学（UD）具有很高的研究活动（卡内基分类），并且大部分活动都集中在生物医学研究的潜水领域。特别是艺术与科学学院以及工程学院，很大程度上取决于组织，细胞和生物材料研究的先进成像工具。 UD通过既定的多用户成像核心设施最大程度地利用了这些仪器，该设施负责维护，调度设备使用，培训用户并就高级技术项目进行咨询或开发新成像技术。我们在UD（LSM510 VIS共聚焦显微镜）中获得的最早的共聚焦显微镜之一是在UD的特拉华生物技术研究所（DBI）生物成像中心中安装的。由于图像质量差，该仪器现在已有15年的历史，并且不再使用，并且制造商截至2009年9月不再提供服务合同。此应用程序是一种重新提交，要求资金替换该过时的乐器，而该工具不再满足我们用户社区的需求，而LSM710倒置的倒置插孔显微镜与环境室。拟议的系统将位于DBI的显微镜套件中，并由生物成像中心技术人员管理。这将确保使用使用DBI生物成像中心的所有调查人员访问拟议的显微镜，这是UD众多NIH资助的研究项目的关键核心设施。目前，DBI生物成像中心仅具有LSM510倒置的共聚焦显微镜，这些显微镜缺乏实用细胞实验的灵敏度和环境控制。因此，我们被要求使用LSM710倒置的共聚焦显微镜，以支持NIH资助的研究人员研究一系列潜水的生物医学过程，其中包括工程血管移植物，程序性细胞死亡，心血管，生物力学，唾液机械，唾液杂志，声音折叠，折叠式生物兴趣，眼睛发育，眼睛发育，肿瘤，骨骼，骨骼，骨骼和HIV组成。