NER: Acoustic Radiation Pressure Driven Atomic Force Microscope for Fast Imaging and Parallel Sensing of Biological and Chemical Processes at the Nanoscale

NER：声辐射压力驱动原子力显微镜，用于纳米级生物和化学过程的快速成像和并行传感

• 批准号: 0210415
• 负责人: Levent Degertekin
• 金额: $ 9万
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2004-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0210415&HistoricalAwards=false
• 关键词: NER; Acoustic; Radiation; Pressure; Driven

0210415DegertekinThe capability of operating in liquid environments has been one of the key reasons for the atomic force microscope's (AFM) indisputable role in the recent advances in nanoscience and nanotechnology. This capability has not only enabled imaging biological samples and observation of biological and chemical processes at the nanoscale, but also led to the development of many microcantilever-based devices in the area of biosensing and proteomics.The liquid environment presents significant challenges to the operation of the AFM, especially in dynamic imaging modes such as tapping mode, and fast imaging applications. As compared to air, the liquids provide a more efficient coupling medium for mechanical perturbations. Hence regular piezoelectric actuation of the AFM cantilever results in spurious resonant signals due to the liquid filled cavity surrounding the sample and the actuator structure. Several novel actuators, based on magnetic, electrostatic, and thin-film piezoelectric techniques have been developed to solve this problem, but these methods severely limit the type of cantilevers and liquids that can be used for experiments. Furthermore, these methods are not suitable for actuation of individual cantilevers in an array, an important capability required for biosensing applications.This exploratory research proposal aims to remove these important obstacles in the implementation of a versatile AFM for applications in liquids using a novel microcantilever actuation technique. The technique uses the acoustic radiation force generated by collimated high frequency (100-400MHz) acoustic waves directed to the AFM cantilever to actuate the cantilever in the DC-MHz frequency range. Promising initial results using the technique have been recently obtained and presented in the proposal. Based on these results, the following objectives are proposed:-Design and microfabrication of individual and arrays of acoustic radiation pressure (ARP) actuators: The actuators will be fabricated on silicon substrates using a thin Zinc Oxide film to generate acoustic waves around 250MHz and silicon micromachining techniques will be used to fabricate acoustic Fresnel lenses to direct the acoustic beams to AFM cantilevers.-Integration of the actuator to a widely available commercial AFM system: A fluid-cell including an ARP actuator will be manufactured and used on a commercial AFM system with appropriate electronics.-Evaluation of the capabilities and limitations of the integrated actuator: The performance of the ARP actuator for fast imaging, as well as array operation will be tested and compared with conventional methods.-Study of possible adverse effects of the ARP actuator: Interaction of high frequency acoustic waves with biological processes will be explored on several important samples and the actuator design will be improved accordingly. Successful implementation of this project will impact numerous areas of nanoscience and engineering, because it will help researchers in the testing and implementation of innovative ideas and in probing a wider variety of biological and chemical processes at the nanoscale.

0210415Degertekin在液体环境中运行的能力一直是原子力显微镜（AFM）在纳米科学和纳米技术的最新进展中无可争议的作用的关键原因之一。这种能力不仅可以使成像生物学样品和观察纳米级的生物学和化学过程观察，而且还导致了在生物传感和蛋白质组学领域的许多基于微型抗体的设备的开发。液体环境对AFM的运行提出了重大挑战，尤其是在诸如启动模式和快速化模式的动态成像中的运行。与空气相比，液体为机械扰动提供了更有效的耦合介质。因此，由于样品和执行器结构周围的液体充满了液体，AFM悬臂的定期压电致致激发会导致虚假共振信号。已经开发了基于磁性，静电和薄膜压电技术的几种新型执行器来解决此问题，但是这些方法严重限制了可用于实验的悬臂和液体的类型。此外，这些方法不适合在阵列中驱动各个悬臂，这是生物传感应用所需的重要能力。该探索性研究提案旨在消除这些重要的障碍，以实施使用新型的MicroCantilever驱动技术在液体中实施多功能AFM。该技术使用直接辐射的声波产生的声波（100-400MHz）的声波，该声波针对AFM悬臂在DC-MHz频率范围内启动悬臂。最近在提案中获得并提出了使用该技术的有希望的初始结果。 Based on these results, the following objectives are proposed:-Design and microfabrication of individual and arrays of acoustic radiation pressure (ARP) actuators: The actuators will be fabricated on silicon substrates using a thin Zinc Oxide film to generate acoustic waves around 250MHz and silicon micromachining techniques will be used to fabricate acoustic Fresnel lenses to direct the acoustic beams to AFM悬臂。执行器与广泛可用的商业AFM系统：包括ARP执行器在内的流体电池将在具有适当电子设备的商业AFM系统上制造并使用。对综合执行器的功能和局限性评估：对快速成像的ARP执行器的性能以及与差异的效果相比，对差异的效果以及可能的方法。执行器：将在几个重要样本上探索高频声波与生物过程的相互作用，并将相应地改进执行器设计。 该项目的成功实施将影响纳米科学和工程的众多领域，因为它将帮助研究人员测试和实施创新思想，并在纳米级的各种生物学和化学过程中进行探索。