Console for an 800/89 DNP Spectrometer

800/89 DNP 光谱仪控制台

• 批准号: 8448486
• 负责人: ROBERT Guy GRIFFIN
• 金额: $ 53.9万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-25 至 2014-06-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8448486
• 关键词: Amyloid Fibrils; Amyloid Proteins; Amyloidosis; Area; Bacteriorhodopsins; Biological; Boston; Communities; Dialysis procedure; Funding; Funding Applicant; Future; Gases; Grant; Investigation; Lipid Bilayers; M2 protein; Magic; Membrane; Membrane Proteins; North America; Nuclear; Phosphatidylinositols; Phosphotransferases; Proteins; Research; Research Project Grants; SH3 Domains; Sampling; Scientist; System; Techniques; Temperature; Testing; Vacuum; Vesicle; Voltage-Dependent Anion Channel; beta-2 Microglobulin; cold temperature; influenzavirus; innovative technologies; instrument; instrumentation; nanodisk; prototype; research study; solid state nuclear magnetic resonance; structural biology

DESCRIPTION (provided by applicant): Funding is requested for the purchase of three essential items that will be used with a 800/89 magnet: (1) a spectrometer console, (2) room temperature shims and (3) a magic angle spinning (MAS) probe with a 1.3 mm stator rotor system. The magnet was delivered in February and is presently at field. In the immediate future the spectrometer will be operated as a high field MAS NMR with low temperature capabilities. However, in the long term it will be converted to an 800 MHz/527 GHz DNP instrument. With funds from another grant we purchased a probe capable of performing experiments in the 90-300 K range and will be used to examine a variety of temperature dependent phenomena in cryoprotected membrane protein and amyloid protein samples. The probe has a vacuum jacketed dewar, sample eject capabilities, and includes a waveguide for dynamic nuclear polarization (DNP) experiments at 527 GHz. Research projects include the following investigations of membrane and amyloid proteins: (1) bacteriorhodopsin (bR) photocycle intermediates in native membranes and (2) nanodiscs, (3) the voltage dependent anion channel (VDAC) in lipid bilayers, (4) the M2 protein from influenza virus, (5) gas vesicles which form a proteinaceous membrane, and amyloid fibrils formed from (6) phosphatidyl-inositol-3-kinase SH3 domain (PI3- SH3), (7) beta-2-microglobulin associated with dialysis related amyloidosis, and (8) the CsgA protein, from Curli. In addition, this will be a prototype instrument performing DNP experiments at 800/527 and will (9) be used to test new biradical polarizing agents, (10) develop high field 17O MAS experiments and (11) new techniques for dipole recoupling at high ?r/2?. The 800/89 will be part of a cluster of instrumentation that serves a community of scientist performing high field biological NMR in the Boston area and North America. It will alleviate a pressing need for additional capabilities for low temperature and high field DNP/ MAS experiments. Further, the instrument will have a significant impact on research since it will employ a number of innovative technologies that are not available elsewhere. For example, the combination of the LT-MAS probe with the 800/89 will be one of the few widebore, low temperature solid state NMR instruments available worldwide and the addition of a 527 GHz gyrotron will make the system a unique DNP spectrometer in North America. It will therefore fill an important void in structural biology.

描述（由申请人提供）：请求资金购买将与 800/89 磁铁一起使用的三个基本物品：(1) 光谱仪控制台，(2) 室温垫片和 (3) 魔角旋转（ MAS）探头带有 1.3 mm 定子转子系统。该磁铁已于二月交付，目前已投入使用。在不久的将来，该光谱仪将作为具有低温功能的高场 MAS NMR 运行。然而，从长远来看，它将转换为 800 MHz/527 GHz DNP 仪器。利用另一笔赠款的资金，我们购买了能够在 90-300 K 范围内进行实验的探针，并将用于检查冷冻保护膜蛋白和淀粉样蛋白样品中的各种温度依赖性现象。该探头具有真空夹套杜瓦瓶、样品弹出功能，并包括用于 527 GHz 动态核极化 (DNP) 实验的波导。研究项目包括对膜和淀粉样蛋白的以下研究：(1) 天然膜中的细菌视紫红质 (bR) 光周期中间体和 (2) 纳米圆盘，(3) 脂质双层中的电压依赖性阴离子通道 (VDAC)，(4) M2来自流感病毒的蛋白质，（5）形成蛋白质膜的气体囊泡，以及由（6）形成的淀粉样原纤维磷脂酰肌醇 3 激酶 SH3 结构域 (PI3-SH3)，(7) 与透析相关淀粉样变性相关的 β-2-微球蛋白，以及 (8) CsgA 蛋白，来自 Curli。此外，这将是在 800/527 下执行 DNP 实验的原型仪器，并将 (9) 用于测试新的双自由基偏振剂，(10) 开发高场 17O MAS 实验，以及 (11) 在高场偶极子重新耦合的新技术?r/2?。 800/89 将成为为科学家群体服务的仪器集群的一部分 在波士顿地区和北美进行高场生物核磁共振。它将缓解低温和高场 DNP/MAS 实验对附加功能的迫切需求。此外，该仪器将对研究产生重大影响，因为它将采用许多其他地方没有的创新技术。例如，LT-MAS 探头与 800/89 的组合将成为全球范围内为数不多的宽口径、低温固态 NMR 仪器之一，并且添加 527 GHz 陀螺仪将使该系统成为北方地区独特的 DNP 波谱仪。美国。因此，它将填补结构生物学的一个重要空白。