Q-band Upgrade to an X-band Pulsed EPR spectrometer

Q 波段升级为 X 波段脉冲 EPR 光谱仪

• 批准号: 10177244
• 负责人: ROBERT Guy GRIFFIN
• 金额: $ 92.02万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-15 至 2024-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177244
• 关键词: 2,4-Dinitrophenol; Address; Anesthesia procedures; Area; Bacterial Proteins; Biochemistry; Biological; Boston; Chemistry; Collagen; Communicable Diseases; Complement; Complex; Electron Nuclear Double Resonance; Electrons; Enzymes; Health; Human; Immunity; Investigation; Medical; Membrane; Membrane Proteins; Methods; Microscope; Minor; Molybdoferredoxin; Motivation; Nitrogenase; Nuclear Magnetic Resonance; Physiologic pulse; Polysaccharides; Proteins; Rain; Research; Research Personnel; Research Project Grants; Resolution; Structure; Viral Proteins; X-Ray Crystallography; improved; instrument; medical schools; metalloenzyme; multidisciplinary; nervous system disorder; structural biology

Summary. A continuous wave and pulsed electron paramagnetic (EPR) spectrometer operating at 34 GHz (Q-band) is requested by investigators at MIT Chemistry, Harvard Medical School, Harvard Chemistry and Brandeis Biochemistry. The instrument’s capabilities include pulsed ENDOR and ELDOR. The primary motivation for the Q-band instrument is the much improved sensitivity and resolution available at 34 GHz as compared to our current X-band (9 GHz) spectrometer. This improved sensitivity and resolution will permit us to address many problems which at the moment are beyond our scientific reach. The instrument will complement high-end X-ray crystallography, nuclear magnetic resonance (NMR), and cryoelectron microscope (cryoEM) capabilities in the Boston area to enable multidisciplinary structural biology and mechanistic biochemistry investigations of questions that are of great biological and medical importance. Research projects from six major user groups (Griffin, Nocera, Nolan, Pandelia, Raines, and Suess) and those from another three minor groups (Betley Keissling, and Wagner) are included in the proposal. The instrument will advance the following health-related research: 1) ENDOR and ESSEM studies of reactive intermediates in radical SAM enzymes, metalloenzymes involved in human immunity, and polynuclear Fe complexes that mimic the nitrogenase FeMo-cofactor; 2) pulsed EPR/DNP methods aimed at developing methods to dynamically polarize proteins at high fields and 3) DEER studies of membrane and soluble proteins, collagen, and polysaccharide secondary structure;. We anticipate that further projects targeting membrane proteins important for example in neurological disease and anesthesia; and viral and bacterial proteins important in infectious disease will appear during the lifetime of the spectrometer.

概括。 工作频率为 34 GHz 的连续波和脉冲电子顺磁 (EPR) 光谱仪 （Q 波段）应麻省理工学院化学系、哈佛医学院、哈佛大学的研究人员的要求 化学和布兰迪斯生物化学 该仪器的功能包括脉冲 ENDOR 和 ELDOR。Q 波段仪器的主要动机是大大提高了灵敏度和 与我们当前的 X 波段 (9 GHz) 光谱仪相比，可在 34 GHz 下获得分辨率。 提高灵敏度和分辨率将使我们能够解决目前存在的许多问题 该仪器将补充高端 X 射线晶体学， 核磁共振 (NMR) 和冷冻电子显微镜 (cryoEM) 功能 波士顿地区将实现多学科结构生物学和机械生物化学 对具有重大生物学和医学重要性的问题进行调查。 来自六个主要用户组（Griffin、Nocera、Nolan、Pandelia、Raines 和 Suess）以及来自 另外三个小团体（贝特利·凯斯林和瓦格纳）也包含在提案中。 该仪器将推进以下健康相关研究：1) ENDOR 和 ESSEM 研究 自由基 SAM 酶、参与人体免疫的金属酶中的反应中间体，以及 模拟固氮酶 FeMo 辅因子的多核 Fe 复合物 2) 脉冲 EPR/DNP 方法； 旨在开发在高场动态极化蛋白质的方法和 3) DEER 研究 膜和可溶性蛋白质、胶原蛋白和多糖二级结构； 预计针对神经学等领域重要的膜蛋白的进一步项目 疾病和麻醉；以及在传染病中重要的病毒和细菌蛋白将会出现 在光谱仪的使用寿命期间。