U.S.-Canada Winter School on Biomolecular Solid State NMR

美国-加拿大生物分子固态核磁共振冬季学校

• 批准号: 9398504
• 负责人: ROBERT Guy GRIFFIN
• 金额: $ 1万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9398504
• 关键词: Amyloid Fibrils; Area; Arts; Biochemical; Biological; Biophysics; Calendar; Canada; Chemistry; Collaborations; Communities; Complex; Computer software; Cryoelectron Microscopy; Data; Development; Education; Electron Spin Resonance Spectroscopy; Electronics; Emerging Technologies; Event; Exposure to; Faculty; Feedback; Flowers; Fostering; Future; Investigation; Isotope Labeling; Knowledge; Magnetic Resonance; Magnetic Resonance Imaging; Membrane; Membrane Proteins; Methods; Modernization; Molecular Biology Techniques; Nuclear; Peptides; Physiologic pulse; Postdoctoral Fellow; Preparation; Principal Investigator; Problem Sets; Proteins; Quantum Mechanics; Renaissance; Research; Research Personnel; Research Project Grants; Resistance; Resolution; Sampling; Schools; Scientist; Series; Solid; Structural Models; Students; System; Techniques; Training; X ray diffraction analysis; X-Ray Diffraction; amyloid structure; biological systems; biophysical techniques; computerized data processing; experimental study; graduate student; instrumentation; lectures; mathematical theory; meetings; microwave electromagnetic radiation; multidisciplinary; next generation; pedagogy; peptide structure; protein expression; protein structure; radio frequency; response; simulation; simulation software; skills; solid state nuclear magnetic resonance; structural biology; success; symposium; synergism; theories

Project Summary In the past 10-15 years, solid state NMR (SSNMR) has emerged as a technique that yields important structural data on systems not amenable to examination with either X-ray diffraction, cryo electron microscopy or solution NMR, presently the three pillars of structural biology. In particular, SSNMR experiments have recently produced the first atomic resolution structures of amyloid fibrils, which have been resistant to detailed investigation by other techniques because they are sparingly soluble and do not crystallize in their biologically relevant state. Similar statements are applicable to membrane proteins. These recent successes are a result of fundamental and practical improvements in spectroscopic and sample preparation methods and instrumentation. Concurrently, technology emerging from developments in SSNMR, for example Average Hamiltonian and Floquet theory, has influenced other areas of research such as solution NMR and magnetic resonance imaging. Finally, the renaissance in sensitivity enhancement via dynamic nuclear polarization (DNP) originally intended for SSNMR experiments is finding wide application in solution NMR and imaging and is further integrating high field electron paramagnetic resonance (EPR) and NMR into a single manifold. In order to rapidly propagate these advances to the scientific community we propose to hold the Winter School on Biomolecular Solid State NMR in Stowe, VT January 7-12, 2018 with the aim of developing the workforce for this flourishing scientific area. The principal component of this school will be a series of lectures on fundamental and advanced topics by leading researchers in the field. Topics will include basic aspects of the mathematical theory of modern SSNMR, specific state-of-the-art experimental techniques, methods for preparation of isotopically labeled proteins, data processing and numerical simulation, the development of structural models from SSNMR data, etc. The lectures will be supplemented by problem sets and hands-on demonstrations of software (SpinEvolution, SIMPSON, GAMMA, etc.) currently used to simulate pulsed NMR experiments important for biological solids. Attendance at this school will train graduate and postdoctoral students in the essential techniques and concepts, greatly enhancing the expertise they would otherwise obtain through research projects in their own groups. The Winter School is being organized in response to the very positive feedback that we received from the students and postdoctorals that attended the school in January 2016. As a result we believe the Winter School an extremely important pedagogical event for the blossoming biological NMR community, and differs significantly from the plethora of other meetings on the calendar.

项目概要 在过去 10-15 年中，固态核磁共振 (SSNMR) 已成为一种可产生 系统的重要结构数据不适合用 X 射线衍射、低温电子检查 显微镜或溶液核磁共振，目前是结构生物学的三大支柱。特别是，SSNMR 最近的实验产生了淀粉样原纤维的第一个原子分辨率结构，该结构已被 难以通过其他技术进行详细研究，因为它们是微溶的并且不 以其生物学相关的状态结晶。类似的说法也适用于膜蛋白。这些 最近的成功是光谱和样品方面基本和实际改进的结果 制备方法和仪器。与此同时，随着技术的发展而出现的技术 SSNMR，例如平均哈密顿量和 Floquet 理论，影响了其他研究领域，例如 如溶液核磁共振和磁共振成像。最后，灵敏度增强的复兴 最初用于 SSNMR 实验的动态核极化 (DNP) 正在得到广泛应用 溶液核磁共振和成像，并进一步集成高场电子顺磁共振 (EPR) 和核磁共振成一个单一的流形。 为了将这些进步迅速传播给科学界，我们建议举办 生物分子固态核磁共振冬季学校将于 2018 年 1 月 7 日至 12 日在佛蒙特州斯托举行，目的是 为这个蓬勃发展的科学领域培养劳动力。这所学校的主要组成部分将是 由该领域的领先研究人员举办的关于基础和高级主题的系列讲座。主题将 包括现代 SSNMR 数学理论的基本方面、具体的最先进的实验 同位素标记蛋白质的制备技术、方法、数据处理和数值模拟 模拟、从SSNMR数据开发结构模型等。讲座将得到补充 通过问题集和软件实践演示（SpinEvolution、SIMPSON、GAMMA 等） 目前用于模拟对生物固体很重要的脉冲核磁共振实验。出席本次 学校将对研究生和博士后学生进行基本技术和概念方面的培训，极大地 增强他们原本可以通过自己小组的研究项目获得的专业知识。 冬季学校的组织是为了回应我们从学校收到的非常积极的反馈 2016 年 1 月就读该校的学生和博士后。因此，我们相信冬季 对于蓬勃发展的生物 NMR 社区来说，学校是一项极其重要的教学活动，并且不同 与日历上过多的其他会议相比，这显着。