Center for Magnetic Resonance

磁共振中心

基本信息

批准号：
7694850
负责人：
ROBERT Guy GRIFFIN
金额：
$ 3.21万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
1976
资助国家：
美国
起止时间：
1976-05-01 至 2009-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7694850
关键词：
Amplifiers Amyloid Amyloid Fibrils Amyloid Proteins Appendix Area Arts Bacteriorhodopsins Biological Boston Cell Nucleus Collaborations Complex Computers Development Development Plans Electron Nuclear Double Resonance Endopeptidases Enzyme Inhibitor Drugs Enzyme Inhibitors Enzymes Equipment Facility Construction Funding Category Faculty Free Radicals Frequencies Funding Gases Goals Grant Housing Human Resources Individual Industry Institution Investigation Ions Joints Journals Label Laboratories Letters Liquid substance Lytic Magic Magnetic Resonance Maintenance Measurement Measures Membrane Membrane Proteins Metals Methodology Methods NMR Spectroscopy New England Noise Nuclear Nucleic Acids Numbers Operative Surgical Procedures Peptide Hydrolases Performance Physiologic pulse Principal Investigator Production Protein NMR Spectroscopy Proteins Protocols documentation Publications Pulse taking Purpose Recombinants Request for Proposals Research Research Personnel Research Project Grants Resolution Ribonucleotide Reductase Sampling Scheme Services Signal Transduction Solid Solutions Spectrum Analysis Spin Labels Structure Students Superoxide Dismutase System Techniques Technology Time Torsion Training Vesicle Work base cold temperature computerized data processing improved instrument instrumentation interest medical schools next generation novel programs protein aggregate research study solid state success

项目摘要

This proposal requests continued support for the MIT/Harvard Center for Magnetic Resonance (CMR) located at the Francis Bitter Magnet Laboratory, MIT (Grant EB0020261RR-00995). During the coming fiveyear period we plan significant upgrades of existing equipment, acquisition of new instrumentation, and research initiatives in several new areas. (1) The cluster of NMR and EPR spectrometers currently operating in the facility will continue to be improved with new transmitters, receivers, pulse programmers, computers, etc. (2) We anticipate that the facility will expand with the addition of five instruments: a 900 MHz spectrometer for solution and solid state NMR to be operated as a national facility; a 750 MHz/89 mm magnet and console for the study of amyIoid and membrane proteins; a 800/52 spectrometer for solution NMR; a 460 GHz/700 MHz spectrometer for high field dynamic nuclear polarization experiments; a 140 GHz, 100 watt gyroamplifier for pulsed EPR and DNP experiments (3) In collaboration with industry we purpose the development of a tunable 330 GHz gyrotron oscillator and low temperature MAS probes that can be used to perform DNP experiments with existing 500 MHz/89 mm magnets. This will enable DNP experiments to be transferred to other laboratories. (4) New and continuing areas of interest in the core research program include: (a) high resolution solution NMR spectroscopy of proteins and protein/nucleic acid complexes, (b) structural studies of solids via dipolar recoupling techniques, (c) structural studies of large proteins including enzyme/inhibitor complexes, amyloid, and membrane, (d) CW and pulsed dynamic nuclear polarization, and (e) high frequency CW and pulsed EPR and ENDOR studies of paramagnetic enzymes and enzyme/inhibitor complexes. (5) Significant collaborative research efforts are planned for solution and solid state NMR and high frequency EPR. (6) The service component of the facility remains an important function as is evident by the 19 proposals from outside investigators who have or plan to use the facility frequently. (7) Our plans for training and dissemination include course work in magnetic resonance, seminars, and publication in scholarly journals, and the dispersal of the DNP technology mentioned above.

该提案请求继续支持麻省理工学院/哈佛大学磁共振中心 (CMR) 位于麻省理工学院弗朗西斯苦磁实验室（授予 EB0020261RR-00995）。未来五年内在此期间，我们计划对现有设备进行重大升级，购买新仪器，以及几个新领域的研究举措。（1）目前NMR和EPR谱仪集群该设施的操作将继续通过新的发射器、接收器、脉冲编程器得到改进， (2) 我们预计该设施将增加五台仪器：a 900 用于溶液和固态核磁共振的 MHz 波谱仪将作为国家设施运行； 750 MHz/89 毫米用于淀粉样蛋白和膜蛋白研究的磁铁和控制台；用于解决方案的 800/52 光谱仪核磁共振；用于高场动态核极化实验的 460 GHz/700 MHz 光谱仪； 140 GHz、100 瓦陀螺放大器，用于脉冲 EPR 和 DNP 实验 (3) 与业界合作，我们目的是开发可调谐 330 GHz 回旋管振荡器和低温 MAS 探头可用于使用现有 500 MHz/89 mm 磁体执行 DNP 实验。这将启用 DNP 实验转移到其他实验室。 (4) 核心领域新的和持续的兴趣领域研究计划包括： (a) 蛋白质和蛋白质/核酸的高分辨率溶液核磁共振波谱配合物，(b) 通过偶极耦合技术进行固体结构研究，(c) 大型材料的结构研究蛋白质，包括酶/抑制剂复合物、淀粉样蛋白和膜，(d) CW 和脉冲动态核极化，以及 (e) 顺磁的高频 CW 和脉冲 EPR 和 ENDOR 研究酶和酶/抑制剂复合物。 (5) 计划开展重大合作研究工作溶液和固态 NMR 以及高频 EPR。 (6) 设施的服务部分仍然是已经或计划使用该功能的外部调查人员提出的 19 项提案就证明了这一点经常设施。 (7) 我们的培训和传播计划包括磁共振课程，研讨会、在学术期刊上发表文章以及上述 DNP 技术的传播。