Cryoprobe repairs and amplifier replacement for the 800 and 750 MHz spectrometers

800 和 750 MHz 光谱仪的冷冻探针维修和放大器更换

• 批准号: 10400474
• 负责人: ROBERT Guy GRIFFIN
• 金额: $ 8.27万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10400474
• 关键词: 2,4-Dinitrophenol; 3-Dimensional; Agreement; Amplifiers; Binding; Caliber; Cholesterol; Communities; Core Protein; Crystallization; Development; Diamond; Disease; Ensure; Equipment; Evolution; Frequencies; Funding; G-Protein-Coupled Receptors; Grant; Helium; Investigation; Isotope Labeling; Label; Laboratories; Libraries; Magic; Magnetic Resonance; Magnetic Resonance Spectroscopy; Measures; Membrane Proteins; Methods; Molecular; Morphologic artifacts; Motion; Nature; Nuclear; Nucleic Acids; Pharmacologic Substance; Physiologic pulse; Procedures; Production; Protein Dynamics; Proteins; Request for Proposals; Research; Resolution; Sampling; Schedule; Seeds; Side; Signal Transduction; Site; Structure; System; Techniques; Technology; Temperature; Tertiary Protein Structure; Thermal Conductivity; Time; Tyrosine; Vertebral column; base; biological systems; cohort; cost; cryogenics; design; dipole moment; experimental study; improved; instrument; instrumentation; magnetic dipole; magnetic field; microwave electromagnetic radiation; next generation; optimal control theory; protein function; protein structure; repaired; structural biology; theories; transmission process

Project Summary Overall The Overall section of the P41 presents a summary of the four TR&D projects which are part of the proposal. These include TR&D 1 – New instrumentation for magnetic resonance TR&D 2 – High-field and fast-MAS methods for biomolecular structure and dynamics TR&D 3 – Next generation solution NMR methods TR&D 4 -- Advanced Magic Angle Spinning NMR methods The specific aims of each of these projects is described and a summary of the contents in presented in more detail. In addition, we provide a rationale for the P41 center which includes the cost and size of present day instrumentation, the unique requirements for instruments and the sophistication of magnetic resonance spectroscopy. We also provide a table enumerating the equipment presently available for the CMR and a list of the new instrumentation that will be constructed during the five years of the proposed grant.

项目概要 全面的 P41 的总体部分总结了四个 TR&D 项目，它们是 提案的一部分。 TR&D 1 – 新型磁共振仪器 TR&D 2 – 用于生物分子结构和动力学的高场和快速 MAS 方法 TR&D 3 – 下一代解决方案 NMR 方法 TR&D 4——先进的魔角旋转核磁共振方法 描述了每个项目的具体目标并总结了内容 更详细地介绍了。 此外，我们还提供了 P41 中心的基本原理，其中包括成本和规模 当今的仪器仪表、对仪器的独特要求和复杂性 磁共振波谱。 我们还提供了一个表格，列出了目前可用于 CMR 的设备和 将在拟议的五年内建造的新仪器清单 授予。

项目成果

Membrane-induced tau amyloid fibrils.

膜诱导的 tau 淀粉样原纤维。

• 发表时间: 2023-04-28
• 影响因子: 5.9
• 作者: El Mammeri, Nadia;Gampp, Olivia;Duan, Pu;Hong, Mei
• 通讯作者: Hong, Mei

Hexamethylene amiloride binds the SARS-CoV-2 envelope protein at the protein-lipid interface.

六亚甲基阿米洛利在蛋白质-脂质界面结合 SARS-CoV-2 包膜蛋白。

• 发表时间: 2023-10
• 作者: Somberg, Noah H;Medeiros;Jo, Hyunil;Wang, Jun;DeGrado, William F;Hong, Mei
• 通讯作者: Hong, Mei

The Cytoplasmic Domain of the SARS-CoV-2 Envelope Protein Assembles into a β-Sheet Bundle in Lipid Bilayers.

SARS-CoV-2 包膜蛋白的细胞质结构域组装成脂质双层中的 β-片束。

• 发表时间: 2023-03-01
• 影响因子: 5.6
• 作者: Dregni, Aurelio J;McKay, Matthew J;Surya, Wahyu;Queralt;Medeiros;Wang, Harrison K;Aguilella, Vicente;Torres, Jaume;Hong, Mei
• 通讯作者: Hong, Mei

Golgi-localized putative S-adenosyl methionine transporters required for plant cell wall polysaccharide methylation.

植物细胞壁多糖甲基化所需的高尔基体定位的假定 S-腺苷甲硫氨酸转运蛋白。

• 发表时间: 2022-06
• 影响因子: 18
• 作者: Temple, Henry;Phyo, Pyae;Yang, Weibing;Lyczakowski, Jan J;Echevarría;Yakunin, Igor;Parra;Terrett, Oliver M;Saez;Dupree, Ray;Orellana, Ariel;Hong, Mei;Dupree, Paul
• 通讯作者: Dupree, Paul

High fidelity sampling schedules for NMR spectra of high dynamic range.

高动态范围 NMR 谱的高保真采样方案。

• 发表时间: 2022-06
• 作者: Hyberts, Sven G;Wagner, Gerhard
• 通讯作者: Wagner, Gerhard