PLANNING AND EVALUATION

规划与评估

• 批准号: 7821305
• 负责人: MARTIN D ABELOFF
• 金额: $ 6.02万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7821305
• 关键词: Autopsy; Bladder; Brain; Breast; Cancer Center; Cancer Center Support Grant; Cell Line; Cervix Uteri; Chromosome abnormality; Clinical Trials; Common Data Element; Computer software; Custom; Data; Databases; Development; Diagnosis; Esophagus; Evaluation; Funding; Future; Gallbladder; Gene Expression; Harvest; Histology; Human; Human Development; Image; Image Analysis; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Information Technology; Internet; Laboratories; Liver; Manuscripts; Microarray Analysis; Microscope; Normal tissue morphology; Online Systems; Operative Surgical Procedures; Ovary; Pancreas; Pathology; Prostate; Prostatic Neoplasms; Publishing; Reproduction spores; Research; Research Personnel; Resolution; Resource Sharing; Salivary Glands; Scanning; Screening procedure; Secure; Security; Services; Slide; Software Tools; Specimen; Speed; Spottings; Staining method; Stains; Technology; Thyroid Gland; Tissue Microarray; Tissue Sample; Tissues; Tumor Tissue; Universities; Update; Xenograft procedure; animal tissue; anticancer research; computerized; design; digital imaging; improved; instrumentation; member; oncology; open source

Tissue microarrays (TMAs) allow rapid screening of gene expression or chromosomal alterations from a large number of tissue samples on a single microscope slide. The TMA Core at Johns Hopkins was initially funded by the Prostate SPORE but has grown into a user-supported University Service Center/Shared Resource. The facility has constructed 398 TMAs for 26 investigators containing 53,304 tissue cores from tumors from the prostate, breast, pancreas, gall bladder, cervix, ovary, brain, liver, urinary bladder, salivary glands, esophagus, head and neck, thyroid and various cell lines. Users Have published 50 manuscripts involving JHU TMAs since 2003. Instrumentation is available to automatically capture high resolution digital images of TMA spots and perform semi-automated quantitative image analysis. The current number of composite TMA images stored server is 104,059 from 84 different TMAs. Users have access to a set of Open Source web-based software tools and backend database (TMAJ) that were developed to facilitate the all facets of research involving TMA technology from TMA design, data entry, image diagnosis, analysis, and publishing on the Internet. The Specific Aims of this Shared Resource are to support and speed translational cancer research by : 1) continuing to produce TMAs for Cancer Center members and others using human surgical and autopsy specimens, human specimens from clinical trials, xenografts, animal tissue specimens and cell lines as needed; 2) providing a center for quantitative TMA image analysis of immunohistochemistry, in situ hybridization and immunofluorescence; 3) to continue to evaluate and update new TMA construction and imaging platforms; 4) to enhance our information technology platform to improve ease of use, compliance with emerging CaBIG standards, including common data elements, and integration of new image analysis technologies with TMAJ.

组织微阵列（TMA）允许快速筛选基因表达或染色体改变 在单个显微镜载玻片上大量的组织样品。约翰·霍普金斯（Johns Hopkins）的TMA核心最初是 由前列腺孢子资助，但已成长为用户支持的大学服务中心/共享 资源。该设施已为26位研究人员构建了398个TMA 来自前列腺，乳房，胰腺，胆囊，子宫颈，卵巢，大脑，肝脏，膀胱，唾液的肿瘤 腺体，食道，头颈，甲状腺和各种细胞系。用户已经发布了50个手稿 自2003年以来涉及JHU TMA。仪器可自动捕获高分辨率数字 TMA斑点的图像并执行半自动化的定量图像分析。当前的数量 来自84种不同TMA的复合TMA图像存储的服务器为104,059。用户可以访问一套开放 基于网络的基于Web的软件工具和后端数据库（TMAJ），这些工具是为了促进所有人的开发 TMA设计，数据输入，图像诊断，分析和 在互联网上发布。该共享资源的具体目的是支持和速度翻译 癌症研究作者：1）继续使用人类为癌症中心成员和其他人生产TMA 手术和尸检标本，临床试验的人类标本，异种移植物，动物组织标本 和细胞系； 2）为免疫组织化学的定量TMA图像分析提供一个中心， 原位杂交和免疫荧光； 3）继续评估和更新新的TMA构建 和成像平台； 4）为了增强我们的信息技术平台以提高易用性， 遵守新兴的Cabig标准，包括常见数据元素以及新图像的集成 TMAJ分析技术。