2nd U.S.-Canada Winter School on Biomolecular Solid State NMR

第二届美国-加拿大生物分子固态核磁共振冬季学校

• 批准号: 7806213
• 负责人: ROBERT Guy GRIFFIN
• 金额: $ 0.5万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-01 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7806213
• 关键词: Amyloid Fibrils; Area; Arts; Biochemical; Biological; Canada; Chemistry; Collaborations; Communities; Complex; Computer software; Data; Development; Education; Electron Spin Resonance Spectroscopy; Electronics; Emerging Technologies; Event; Feedback; Flowers; Fostering; Frequencies; Future; Hand; Investigation; Knowledge; Label; Magnetic Resonance; Magnetic Resonance Imaging; Membrane; Methods; Molecular Biology Techniques; Nuclear; Peptides; Physiologic pulse; Preparation; Principal Investigator; Proteins; Quantum Mechanics; Radio; Renaissance; Research; Research Personnel; Research Project Grants; Resistance; Sampling; Schools; Science; Scientist; Series; Simulate; Solid; Solutions; Structural Models; Structure; Students; System; Techniques; Training; X ray diffraction analysis; X-Ray Diffraction; biological systems; computerized data processing; graduate student; lectures; mathematical theory; microwave electromagnetic radiation; multidisciplinary; next generation; protein expression; research study; response; simulation; skills; solid state; solid state nuclear magnetic resonance; structural biology; success; theories

DESCRIPTION (provided by applicant): In the past 5-10 years, solid state NMR (SSNMR) has emerged as a technique that yields important structural data on systems not amenable to examination with either X-ray diffraction or solution NMR, presently the two pillars of structural biology. For example, SSNMR experiments have recently produced some of the first structures of membrane peptides and proteins and the first experimentally-determined structures of amyloid fibrils, which have been resistant to detailed investigation by other techniques because they are insoluble and do not crystallize in their biologically relevant state. These recent successes are a result of fundamental and practical improvements in spectroscopic and sample preparation methods. Concurrently, technology emerging from developments in SSNMR, for example Average Hamiltonian Theory has influenced other areas of research such as solution NMR and magnetic resonance imaging. Finally, the renaissance in sensitivity enhancement via dynamic nuclear polarization (DNP) originally intended for SSNMR experiments is finding wide application in solution NMR and imaging and is further integrating high field electron paramagnetic resonance (EPR) and NMR into a single manifold. In order to rapidly propagate these advances to the scientific community we propose to hold the 2nd Winter School on Biomolecular Solid State NMR in Stowe, VT January 24-29, 2010. The principal component of this school will be a series of lectures on fundamental and advanced topics by leading researchers in the field. Topics will include basic aspects of the mathematical theory of modern SSNMR, assessment of practical advantages of specific state-of-the-art experimental techniques, methods for preparation of isotopically labeled proteins and peptides, data processing and numerical simulation, the development of structural models from SSNMR data, etc. The lectures will be supplemented by problem sets and hands-on demonstrations of software (SPINEVOLUTION, SIMPSON, GAMMA, etc.) currently used to simulate pulsed NMR experiments important for biological solids. Attendance at this school will expose graduate and postdoctoral students to a broad spectrum of essential techniques and concepts, greatly enhancing the more specialized training they would otherwise obtain through specific research projects in their own research groups. This is the 2nd SSNMR school and is being organized in response to the very positive feedback that we received from the students and postdoctorals that attended the 1st school in January 2008. As a result we believe the Winter School an extremely important pedagogical event for the blossoming solid state biological NMR community.

描述（由申请人提供）：在过去 5-10 年中，固态 NMR (SSNMR) 已成为一种技术，可以为不适合用 X 射线衍射或溶液 NMR 检查的系统提供重要的结构数据，目前这两种方法结构生物学的支柱。例如，SSNMR 实验最近产生了膜肽和蛋白质的一些第一个结构以及淀粉样原纤维的第一个实验确定的结构，这些结构一直无法通过其他技术进行详细研究，因为它们在生物学中不溶且不结晶。相关状态。最近的这些成功是光谱和样品制备方法的根本和实际改进的结果。与此同时，SSNMR 发展中出现的技术，例如平均哈密顿理论，已经影响了溶液 NMR 和磁共振成像等其他研究领域。最后，最初用于 SSNMR 实验的动态核极化 (DNP) 增强灵敏度的复兴正在溶液 NMR 和成像中得到广泛应用，并进一步将高场电子顺磁共振 (EPR) 和 NMR 集成到单个流形中。为了快速向科学界传播这些进展，我们建议于 2010 年 1 月 24 日至 29 日在佛蒙特州斯托举办第二届生物分子固态 NMR 冬季学校。该学校的主要内容将是一系列关于基础和生物分子固态 NMR 的讲座。该领域领先研究人员的高级主题。主题将包括现代 SSNMR 数学理论的基本方面、特定最先进实验技术的实际优势评估、同位素标记蛋白质和肽的制备方法、数据处理和数值模拟、结构模型的开发讲座将辅以问题集和软件（SPINEVOLUTION、SIMPSON、GAMMA 等）的动手演示，这些软件目前用于模拟对生物固体很重要的脉冲 NMR 实验。就读这所学校将使研究生和博士后学生接触到广泛的基本技术和概念，极大地增强他们原本可以通过自己研究小组的具体研究项目获得的更专业的培训。这是第二所 SSNMR 学校，其组织是为了响应我们从 2008 年 1 月参加第一所学校的学生和博士后那里收到的非常积极的反馈。因此，我们相信冬季学校对于蓬勃发展的学生来说是极其重要的教学活动。固态生物核磁共振社区。