WNPRC ASSAY SERVICES UNIT

WNPRC 检测服务单位

• 批准号: 8173095
• 负责人: Toni Elaine ZIEGLER
• 金额: $ 16.52万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173095
• 关键词: Accounting; Adolescent; Adult; Alouatta; American; Androgenization; Anthropology; Back; Behavioral; Biogenic Amines; Biological Assay; California; Callithrix; Callithrix jacchus jacchus; Catecholamines; Charge; Client; Clinical; Clinical and Translational Science Awards; Colobus Genus; Communities; Computer Retrieval of Information on Scientific Projects Database; Corpus striatum structure; Corticosterone; Country; Detection; Development; Dopamine; Endocrine; Fee-for-Service Plans; Female; Finland; Funding; Grant; High Pressure Liquid Chromatography; Hormonal; Hormones; Human; Hydrocortisone; Hypothalamic structure; Income; Infant; Institution; International; Lemurs; Macaca mulatta; Maternal Behavior; Measures; Methods; Monkeys; Mus; National Center for Research Resources; Neuroendocrinology; Neurosciences; New York; Ovarian; Oxytocin; Parenting behavior; Phenotype; Physiology; Pituitary Gland; Plasma; Polycystic Ovary Syndrome; Postdoctoral Fellow; Primates; Printing; Progesterone; Programmed Learning; Prolactin; Proteins; Psychology; Psychoneuroendocrinology; Recommendation; Research; Research Personnel; Resource Allocation; Resources; Role; Running; S10 grant; Sampling; Scientist; Serotonin; Serum; Services; Societies; Source; Students; Testosterone; Time; Tissues; Training; Translational Research; United States National Institutes of Health; Universities; Urine; Validation; Variant; Vasopressins; Vitamin D; Vitamin D2; Vitamins; Wisconsin; Work; base; experience; fetal; graduate student; high school; instrumentation; kisspeptin; liquid chromatography mass spectrometry; male; meetings; method development; nonhuman primate; prenatal; reproductive; social; translational study; web site

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To provide core, CTSA clinical translational research, campus and non-host investigators with sophisticated and specialized assay resources and expertise. Progress and Concerns: From January 1, 2009 through December 31, 2009, Assay Services has received $191,949 as income from our charge back accounts for our service and $319 from charging base grant accounts within the Primate Center. We ran 46,342 determinations during this time. Assay Services' new initiatives have been 1) iodinating for other labs for their RIA of protein hormones as a fee for service, 2) development and validation of methods for determining biogenic amines including dopamine and its metabolites and serotonin in striatal tissue, 3) development of methods for measuring dopamine in marmoset pituitary and hypothalamic perfusates 4) developmental work on oxytocin for an ultrasensitive assay, 5) developmental work on oxytocin 6) running human samples as a core lab for the Clinical Translational Science Award at the University of Wisconsin for translational studies, 6) LC/MS development for measuring Vitamin D (1,25 Vitamin D2&3, 25(OH) Vitamin D2&3, 7) development and validation of a kisspeptin assay for rhesus serum. Allocation of Resource Access: Assay Services has an international and national client base. During the past year we ran samples for investigators from many different states and several countries. We primarily provide services for NIH based grants using nonhuman primate samples for hormonal determinations and more recently for translational studies. We submitted a proposal for the NCRR shared instrumentation grant (S10) in March for a LC/MS/MS to enhance our capability to measure the Vitamin Ds simultaneously and other multisteroids. Assay Services has a website that is easily assessable to the scientific community: http://ink.primate.wisc.edu/~assay/assay.php Dissemination: We have many clients who contact us via our website. Additionally, we attend meetings such as the American Society of Primatologists, International Primatological Society, Society of Behavioral Neuroendocrinology, and the Endocrine Society to make the nonhuman primate community aware of our services. We also receive clients from recommendations by previous clients. Training: Assay Services continues to train graduate students, technical support, post doc and scientists to run their own samples as self-service. A postdoctoral student from the Stony Brook University, New York, spent several months validating and analyzing fecal samples from several species of free-ranging lemurs along with a graduate student from the University of Helsinki, Finland. A University of Wisconsin anthropology graduate student trained and ran fecal samples for testosterone and cortisol from Alouatta pigra. A graduate student at UC-Berkeley spent six months training and analyzing samples from red colobus monkeys. A postdoc from Psychology trained in analysis of urine samples for oxytocin and vasopressin. A graduate student with the UW neuroscience program learned HPLC with electrochemical detection for mouse striatal tissue. Another graduate student trained and analyzed serum samples for corticosterone, testosterone and progesterone for Californica mus. Additionally, we have trained two graduate students and a postdoctoral student from Ei Terasawa's research team. Within Assay Services, we trained one undergraduate student for working in the lab and a high school student for summer time training. Assay Services has begun to train technical staff at the California Primate Center to develop plasma catecholamines detection by HPLC electrochemical detection. PUBLICATIONS: *Ziegler TE, Prudom SL, Zahed SR. Variations in male parenting behavior and physiology in the common marmoset. Am J Hum Biol. 2009 Nov-Dec;21(6):739-44. PMID: 19367571 *Abbott DH, Tarantal AF, Dumesic DA. Fetal, infant, adolescent and adult phenotypes of polycystic ovary syndrome in prenatally androgenized female rhesus monkeys. Am J Primatol. 2009 Sep;71(9):776-84.PMID: 19367587 *Dumesic DA, Patankar MS, Barnett DK, Lesnick TG, Hutcherson BA, Abbott DH. Early prenatal androgenization results in diminished ovarian reserve in adult female rhesus monkeys. Hum Reprod. 2009 Sep 9. [Epub ahead of print]PMID: 19740899 *Ziegler TE, Prudom SL, Zahed SR, Parlow AF, Wegner F. Prolactin's mediative role in male parenting in parentally experienced marmosets (Callithrix jacchus). Horm Behav. 2009 Oct;56(4):436-43. Epub 2009 Aug 5. PMID: 19664636 Van Belle S, Estrada A, *Ziegler TE, Strier KB. Social and hormonal mechanisms underlying male reproductive strategies in black howler monkeys (Alouatta pigra). Horm Behav. 2009 Oct;56(4):355-63. Epub 2009 Aug 25.PMID: 19712675 *Saltzman W, Abbott DH. Effects of elevated circulating cortisol concentrations on maternal behavior in common marmoset monkeys (Callithrix jacchus). Psychoneuroendocrinology. 2009 Apr 10. [Epub ahead of print ] PMID: 19362777

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 目标：为核心、CTSA 临床转化研究、校园和非主办研究人员提供先进和专业的检测资源和专业知识。 进展和担忧： 从 2009 年 1 月 1 日到 2009 年 12 月 31 日，Assay Services 从我们的服务退款账户中收到了 191,949 美元的收入，从灵长类动物中心内的收费基础补助金账户中收到了 319 美元的收入。 在此期间，我们进行了 46,342 次测定。 Assay Services 的新举措包括 1) 为其他实验室进行蛋白质激素 RIA 碘化作为服务费用，2) 开发和验证用于测定纹状体组织中生物胺（包括多巴胺及其代谢物和血清素）的方法，3) 开发测量狨猴垂体和下丘脑灌注液中多巴胺的方法 4) 用于超灵敏测定的催产素的开发工作，5)催产素的开发工作 6) 作为威斯康星大学转化研究临床转化科学奖的核心实验室运行人体样本，6) 用于测量维生素 D（1,25 维生素 D2&3、25(OH) 维生素）的 LC/MS 开发D2&3, 7) 恒河猴血清 Kisspeptin 测定的开发和验证。 资源访问的分配： 检测服务拥有国际和国内客户群。 在过去的一年里，我们为来自许多不同州和几个国家的调查人员收集了样本。 我们主要为基于 NIH 的拨款提供服务，使用非人类灵长类动物样本进行激素测定，最近还用于转化研究。 我们于 3 月份提交了一份 NCRR 共享仪器拨款 (S10) 提案，用于 LC/MS/MS，以增强我们同时测量维生素 D 和其他多类固醇的能力。 Assay Services 有一个可供科学界轻松访问的网站： http://ink.primate.wisc.edu/~assay/assay.php 传播： 我们有许多客户通过我们的网站与我们联系。 此外，我们还参加美国灵长类动物学家协会、国际灵长类动物学会、行为神经内分泌学会和内分泌学会等会议，以使非人类灵长类动物界了解我们的服务。 我们还通过以前客户的推荐来接待客户。 训练： 检测服务继续培训研究生、技术支持、博士后和科学家以自助方式运行自己的样品。 纽约石溪大学的一名博士后学生与芬兰赫尔辛基大学的一名研究生花了几个月的时间验证和分析了几种自由放养狐猴的粪便样本。 威斯康星大学人类学研究生对 Alouatta Pigra 的粪便样本进行了训练和检测，以检测睾酮和皮质醇。 加州大学伯克利分校的一名研究生花了六个月的时间训练和分析红疣猴的样本。心理学博士后接受过尿液样本催产素和加压素分析培训。华盛顿大学神经科学项目的一名研究生学习了 HPLC 和小鼠纹状体组织的电化学检测。另一名研究生对加州小鼠的皮质酮、睾酮和黄体酮血清样本进行了培训和分析。 此外，我们还培养了寺泽荣研究团队的两名研究生和一名博士后。 在检测服务中，我们培训了一名本科生在实验室工作和一名高中生进行暑期培训。 Assay Services 已开始培训加州灵长类动物中心的技术人员，以开发通过 HPLC 电化学检测来检测血浆儿茶酚胺的方法。 出版物： *Ziegler TE、Prudom SL、Zahed SR。普通狨猴雄性育儿行为和生理的变化。 Am J Hum Biol。 2009 年 11 月至 12 月；21(6):739-44。 PMID：19367571 *雅培 DH、塔兰塔尔 AF、杜梅西克 DA。产前雄激素化雌性恒河猴的胎儿、婴儿、青少年和成人多囊卵巢综合征表型。我是 J Primatol。 2009 年 9 月；71(9):776-84.PMID: 19367587 *Dumesic DA、Patankar MS、Barnett DK、Lesnick TG、Hutcherson BA、Abbott DH。早期产前雄激素化导致成年雌性恒河猴卵巢储备减少。嗡嗡声再现。 2009 年 9 月 9 日。[打印前的电子版]PMID：19740899 *Ziegler TE、Prudom SL、Zahed SR、Parlow AF、Wegner F。催乳素在有养育经历的狨猴（Callithrix jacchus）的雄性养育中的中介作用。霍姆行为。 2009 年 10 月；56(4)：436-43。电子版 2009 年 8 月 5 日。PMID：19664636 范贝尔 S、埃斯特拉达 A、*齐格勒 TE、斯特里尔 KB。黑吼猴（Alouatta Pigra）雄性生殖策略的社会和激素机制。霍姆行为。 2009 年 10 月；56(4)：355-63。 Epub 2009 年 8 月 25 日.PMID: 19712675 *Saltzman W，雅培 DH。循环皮质醇浓度升高对普通狨猴（Callithrix jacchus）母性行为的影响。精神神经内分泌学。 2009 年 4 月 10 日。[印刷前的电子版] PMID：19362777