SIRNA NANOPARTICLES

SIRNA纳米粒子

• 批准号: 8168579
• 负责人: CYRUS R SAFINYA
• 金额: $ 0.65万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168579
• 关键词: Aminoglycosides; Behavior; Categories; Complex; Computer Retrieval of Information on Scientific Projects Database; Funding; Gene Silencing; Goals; Grant; Home environment; Image; Institution; Lipids; Nature; Phase; Research; Research Personnel; Resources; Roentgen Rays; Small Interfering RNA; Source; Structure; System; United States National Institutes of Health; nanoparticle; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Complexes comprising siRNA and lipidic aminoglycoside derivatives have been recently imaged by Cryo-EM, displaying a typical layered microstructure. In our lab we prepared lipid-siRNA constructs using a variety of lipid systems which have been successfully employed in gene silencing activities. The lipid-siRNA phase behavior appears to be very rich and so far we have identified by X-ray scattering three categories of supramolecular structures. Specifically: i) a lamellar phase obtained with commercially available cationic/non-ionic lipids, ii) a 2D hexagonal structure obtained with multivalent cationic lipids synthesized in our lab, and finally iii) a bicontinuous cubic phase obtained by a combination of a "cubic forming" neutral lipid and "home synthesized" multivalent cationic lipids (as well as commercially available). In addition, we have observed that the nature of the phase has a great impact on SE. We propose Cryo-EM experiments to obtain 2D images of the lipid nanoparticles alone and upon aggregation with siRNA. Our long-term goals are to locate the siRNA domains in the lipid-siRNA aggregate.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 最近，Cryo-Em成像了包含siRNA和脂质氨基糖苷衍生物的复合物，显示出典型的分层微观结构。 在我们的实验室中，我们使用多种在基因沉默活性中成功使用的脂质系统制备了脂质 - 丝娜构建体。脂质 - 丝娜相的行为似乎非常丰富，到目前为止，我们已经通过X射线散射三类超分子结构来识别。 Specifically: i) a lamellar phase obtained with commercially available cationic/non-ionic lipids, ii) a 2D hexagonal structure obtained with multivalent cationic lipids synthesized in our lab, and finally iii) a bicontinuous cubic phase obtained by a combination of a "cubic forming" neutral lipid and "home synthesized" multivalent cationic lipids (as well as市售）。此外，我们已经观察到该阶段的性质对SE具有很大的影响。 我们提出了冷冻EM实验，以单独并与siRNA聚集时获得脂质纳米颗粒的2D图像。我们的长期目标是将siRNA结构域定位在脂质 - siRNA骨料中。