SYNCHROTRON X-RAY STUDIES OF BIOMOLECULAR MATERIALS

生物分子材料的同步辐射 X 射线研究

基本信息

批准号：
8169977
负责人：
CYRUS R SAFINYA
金额：
$ 1.69万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-01 至 2011-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8169977
关键词：
Biopolymers Blood capillaries Chemicals Computer Retrieval of Information on Scientific Projects Database Crystallography Drug Industry Electronics Electrons F-Actin Funding Grant Image Institution Lead Learning Microscopy Microtubules Nature Optics Powder dose form Proteins Research Research Personnel Resources Sampling Source Structure Synchrotrons Techniques United States National Institutes of Health capillary crosslink insight nanometer nanoscale nanoscience neurofilament research study

Biopolymers Blood capillaries Chemicals Computer Retrieval of Information on Scientific Projects Database Crystallography Drug Industry Electronics Electrons F-Actin Funding Grant Image Institution Lead Learning Microscopy Microtubules Nature Optics Powder dose form Proteins Research Research Personnel Resources Sampling Source Structure Synchrotrons Techniques United States National Institutes of Health capillary crosslink insight nanometer nanoscale nanoscience neurofilament research study

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The experiments aim to elucidate the key parameters that control the interactions between cytoskeletal filamentous proteins (the biopolymers neurofilaments, microtubules, and filamentous actin), and lead to hierarchical supramolecular structures on the nanometer to micron scale. The biopolymer assemblies will be characterized in powder and oriented samples using x-ray capillaries and microchannels, respectively. The synchrotron structure studies of oriented assemblies in microchannels should reveal the nature of the ordering of the cross-linking, or, in the case of neurofilaments, protruding sidearm proteins, and provide important insight into the origin of interfilament interactions. The proposal?s unique approach combines synchrotron x-ray scattering, which yields statistically averaged structures in reciprocal space, and real-space imaging with electron and optical microscopy, to probe and solve non-crystalline structures of biomacromolecular assemblies (where protein crystallography techniques do not apply). The learned concepts should lead to the exciting possibility of developing advanced nanometer scale materials for nanoscience applications in the electronic, chemical, and pharmaceutical industries.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。该实验旨在阐明控制细胞骨架丝状蛋白（生物聚合物神经丝，微管和丝状肌动蛋白）之间相互作用的关键参数，并导致纳米尺度上的纳米尺度上的层次上分子结构。生物聚合物组件将分别在粉末和定向样品中使用X射线毛细管和微通道进行表征。微通道中定向组件的同步加速器结构研究应揭示交联的顺序的性质，或者在神经丝的情况下，伸出了侧层蛋白，并对间相互作用的起源提供重要的见解。该提案的独特方法结合了同步子X射线散射，该散射在相互空间中产生统计上平均的结构，以及用电子和光学显微镜进行实空成像，以探测和求解非晶体的生物分子组件的非晶体结构（其中蛋白质晶体学技术不适用）。学识渊博的概念应导致令人兴奋的可能性开发用于电子，化学和制药行业中纳米科学应用的高级纳米尺度材料。