STERICALLY STABILIZED CATIONIC LIPID - DNA COMPLEXES USED IN GENE DELIVERY

空间稳定的阳离子脂质 - 用于基因传递的 DNA 复合物

• 批准号: 8362475
• 负责人: CYRUS R SAFINYA
• 金额: $ 1.93万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362475
• 关键词: Cells; Chromosomes, Artificial, Human; Complex; DNA; Funding; Gene Delivery; Genes; Grant; In Vitro; Knowledge; Lipids; Liposomes; Measures; Microscopy; Molecular; National Center for Research Resources; Principal Investigator; Renaissance; Research; Research Infrastructure; Resources; Source; Therapeutic; Transfection; United States National Institutes of Health; Viral Vector; Virus; base; cost; in vivo; vector

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The research field focusing on virus-free synthetic vectors intended for therapeutic delivery applications, in particular, using cationic liposome based vectors has recently undergone a renaissance. This is due to the non-immunogenicity of the vectors and the potential of transferring large DNA pieces such as sections of about 1Mbps of human artificial chromosome into cells which is not feasible with viral vectors. However, transfection efficiency (TE; a measure of the expression of an exogenous gene that is transferred into cells) of cationic lipid-DNA complexes (CLDCs), remains low compared to viral vectors and only a significant further increase in our knowledge of the interactions between CLDCs and cells will result in in vitro as well as in vivo optimization of TE.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 专注于无病毒的合成媒介的研究领域旨在进行治疗递送 尤其是使用阳离子脂质体载体的应用程序最近进行了A 复兴。这是由于向量的非免疫原性以及 转移大型DNA碎片，例如大约1Mbps人造的部分 染色体进入病毒载体不可行的细胞。但是，转染 效率（TE；用于转移到的外源基因表达的量度 与病毒载体和 我们对CLDC与CLDC与 细胞将导致TE的体外以及体内优化。