Developmental Core

发展核心

• 批准号: 10222674
• 负责人: Kurunthachalam Kannan
• 金额: $ 34.06万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10222674
• 关键词: Adipose tissue; Amniotic Fluid; Biological; Biological Assay; Biological Monitoring; Blood; Chemicals; Chromium; Client; Coupling; Development; Environment; Follicular Fluid; Gas Chromatography; Generations; Goals; Hair; Health; Human; Human Milk; Human Resources; Inductively Coupled Plasma Mass Spectrometry; International; Laboratories; Liquid Chromatography; Liquid substance; Measurement; Measures; Meconium; Methods; Nail plate; Nickel; Organic Chemicals; Placenta; Publications; Quality Control; Rare Earth Metals; Research; Resources; Running; Sampling; Seminal Plasma; Spottings; Suggestion; Technology; Time; Tissues; Tooth structure; Trace Elements; Umbilical Cord Blood; Umbilical cord structure; Validation; Vanadium; analytical method; base; bone; cost; detection limit; environmental chemical; environmental stressor; experience; human tissue; improved; instrumentation; mass spectrometer; novel; novel marker; novel strategies; programs; response biomarker; tandem mass spectrometry; toxic metal

ABSTRACT – DEVELOPMENTAL CORE The overall goal of the Wadsworth Center’s Human Health Exposure Analysis Resource (WC-HHEAR) Developmental Core (Dev. Core) is to capitalize on the existing resources, expertise, and capacity at Wadsworth to develop and validate novel methods and analytical approaches for quantitative measurements of emerging chemicals in biospecimens, i.e., pushing the boundaries of human biomonitoring. Drs. Kannan and Parsons, the MPIs on this proposal, are leaders in their respective fields with more than 700 combined publications. They have spent the last two decades building the human biomonitoring program at Wadsworth – in Dr. Parsons’ Trace Elements Laboratory (TREL) and in Dr. Kannan’s Human Organics Biomonitoring Lab (HOBL). These efforts include assembling the current analytical capability; discovering emerging chemicals in human tissues and fluids; developing and validating new methods of analysis; and supporting the advancement of human health studies and the environment. Both HOBL and TREL have expansive lists of assays and capabilities that are part of the repertoire committed to support the WC-HHEAR targeted analysis resource (TAR). One of the Dev. Core’s functions will be to improve existing methods, requiring less sample volume for analysis, doing more with a limited amount of sample, improving detection limits, increasing sample throughput, reducing costs, and leveraging state-of-the-art technologies as they become available. For example, the proposed Dev. Core will investigate extractions that can be implemented for use on multiple classes of organic chemicals and improving detectability for trace elements using a new generation of technology based on inorganic tandem mass spectrometry. New capabilities will be developed in the Dev. Core and validated according to the international standards for single-laboratory method validation. The Dev. Core will build on its existing expertise with non-traditional matrices to offer more targets in more matrix types and will respond to HHEAR – driven by either discoveries in the untargeted resource, increased demand from HHEAR clients, or suggestions from the HHEAR program office. The transfer of these improvements to current methods will be facilitated by the WC-HHEAR administrative core, where key staff are integrated into both the TAR and Dev. Core. It is only through the progress outlined above that high-quality, quantitative, exposome research can flourish.

摘要 - 发展核心 沃兹沃思中心人类健康暴露分析资源（WC-HHEAR）的总体目标 发展核心（Dev。Core）是利用现有资源，专业知识和能力 沃兹沃思（Wadsworth）开发和验证新方法和分析方法的定量方法 生物测量中新兴化学物质的测量，即推动人类的边界 生物监测。博士。坎南（Kannan）和帕森斯（Parsons）是该提案的MPI，是各自领域的领导者 有700多个合并出版物。在过去的二十年中，他们已经建立了人类 沃兹沃思（Wadsworth）的生物监测计划 - 帕森斯（Parsons）的微量元素实验室（TREL）和博士 坎南的人类有机物生物监测实验室（HOBL）。这些努力包括组装电流 分析能力；在人体组织和液体中发现新兴的化学物质；开发和 验证新的分析方法；并支持人类健康研究的进步和 环境。 HOBL和TREL都有额外的测定和功能清单 曲目致力于支持WC-HHEAR目标分析资源（TAR）。开发者之一。 Core的功能将是改善现有方法，需要更少的样本量进行分析，执行 有限的样本，改善检测限，增加样品吞吐量，减少更多的样品 成本，并利用最先进的技术可用。例如，提议 开发核心将调查可在多种有机类别上使用的提取物 化学物质和改善痕量元素的可检测性，使用新一代技术基于 无机串联质谱。开发人员将开发新的功能。核心和验证 根据单位实验室方法验证的国际标准。开发人员。核心将建立 关于其与非传统物品的现有专业知识，以提供更多矩阵类型的目标，并将 响应HHEAR - 在未靶向资源中发现的任何一个发现，增加的需求。 HHEAR客户或HHEAR计划办公室的建议。这些改进转移到 当前的方法将由WC-HHEAR行政核心准备，其中关键人员被整合在一起 进入焦油和开发。核。只有通过上面概述的高质量的进展， 定量，外向研究会浮动。