Targeted Analysis

针对性分析

• 批准号: 10836245
• 负责人: Kurunthachalam Kannan
• 金额: $ 83.64万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10836245
• 关键词: Targeted; Analysis

PROJECT SUMMARY / ABSTRACT – TARGETED The overall objective of the Targeted Analysis Resource (TAR) within the Wadsworth Center’s Human Health and Exposure Analysis Resource (WC-HHEAR) is to provide access to state-of-the-art, analytical facilities to HHEAR clients conducting studies into environmental exposures and the effects of environmental contaminants on human health. The proposed TAR will leverage the Center’s extensive infrastructure, expertise, and reputation as a world-class biomonitoring laboratory that has been built over several decades in the Department of Environmental Health Sciences (DEHS). Within DEHS two analytical lab groups conduct targeted analyses for environmental contaminants: the Trace Elements Lab (TREL) and the Human Organics Biomonitoring Lab (HOBL). Dr. Patrick Parsons is the Director of DEHS overseeing the TREL, while Dr. Kurunthlachalam Kannan is Deputy Director of DEHS overseeing the HOBL. Drs. Parsons and Kannan (the MPIs of this proposal) have more than 65 years of combined experience in analytical chemistry applied to measuring environmental contaminants in human tissues and fluids. The HOBL analyzes biospecimens for 30 classes of trace organic contaminants totaling hundreds of analytes including, but not limited to, polychlorinated biphenyls, polycyclic aromatic hydrocarbons, perfluorinated compounds, brominated flame retardants, pesticides (e.g., organophosphate, organochlorine, synthetic pyrethroid and neonicotinoid insecticides), phthalates (including metabolites, replacements and emerging chemicals), drugs (e.g., opioids), biomarkers of stress (e.g., oxidative stress markers of DNA, protein and lipids), life style biomarkers (e.g., tobacco smoke) and nutritional biomarkers (e.g., phytoestrogens, micronutrients). The TREL group analyzes biospecimens for up to 22 trace elements that including toxic (lead, cadmium, mercury and arsenic) and essential (copper, selenium, zinc) elements in whole blood, urine, and serum/plasma, plus the 16 rare earth elements, which includes the lanthanides series. Both HOBL and TREL have extensive expertise analyzing non-traditional matrices such as placenta, cord blood, baby teeth, dried blood spots, hair, nails, amniotic fluid, breast milk, saliva, and other tissues. The TAR will capitalize on Wadsworth’s international reputation and leadership to promote harmonization of laboratory analyses for biomonitoring and exposure analysis based on rigorous quality control which is evidenced by 1) organizing external proficiency testing programs and educational interlaboratory studies (for emerging assays); 2) providing subject matter expertise through disseminating standard operating protocols; 3) offering hands-on training for staff from other laboratories; and 4) producing well-characterized quality control and reference materials that are available to the wider scientific community. The wealth and breadth of experience, knowledge, skills and reputation that WC-HHEAR has garnered over the years, including its successful participation in CHEAR as a TAR, will enable the successful and smooth implementation of HHEAR exposomics for the range of exposures that require rigorous analytical quality control.

项目摘要 /摘要 - 针对性 沃兹沃思中心人类健康中目标分析资源（TAR）的总体目标 和暴露分析​​资源（WC-HHEAR）是为了提供对最先进的分析设施的访问权限 HHEAR客户进行环境暴露和环境污染物的影响 关于人类健康。拟议的焦油将利用该中心广泛的基础设施，专业知识和 作为世界一流的生物监测实验室的声誉，该实验室已在该部门建立了数十年 环境卫生科学（DEHS）。在DEHS中，两个分析实验室组进行了针对性的分析 对于环境污染物：痕量元素实验室（TREL）和人类有机物生物监测实验室 （HOBL）。帕特里克·帕森斯（Patrick Parsons）博士是DEHS的主任，负责TREL，而Kurunthlacharam Kannan博士 是DEHS监督Hobl的副主任。博士。 Parsons和Kannan（该提案的MPI） 超过65年的分析化学经验应用于测量环境 人体组织和流体中的污染物。 HOBL分析了30种痕量有机的生物测量 总计数百种分析物的污染物，包括但不限于多氯苯基，多环芳烃 芳族碳氢化合物，全氟化化合物，溴化阻燃剂，农药（例如， 有机磷酸盐，有机氯，合成拟除虫毒和新烟碱杀虫剂），邻苯二甲酸盐（包括 代谢物，替代品和新兴化学物质），药物（例如阿片类药物），胁迫的生物标志物（例如氧化 DNA，蛋白质和脂质的应力标记），生活方式生物标志物（例如烟草烟）和营养生物标志物 （例如，植物雌激素，微量营养素）。 TREL组分析了多达22个微量元素的生物测量 包括有毒（铅，镉，汞和砷）和必不可少的（铜，硒，锌）元素 血液，尿液和血清/血浆，以及16个稀土元素，其中包括兰塔尼德斯系列。两个都 HOBL和TREL具有广泛的专业知识，分析了非传统物质，例如胎盘，脐带血，婴儿 牙齿，干斑，头发，指甲，羊水，母乳，唾液和其他组织。焦油将大写 关于沃兹沃思的国际声誉和领导地位，以促进实验室分析的协调 基于严格质量控制的生物监测和暴露分析​​，这可以通过1）组织 外部能力测试计划和教育介入研究（用于新兴测定）； 2）提供 主题专业知识通过传播标准运营协议； 3）提供动手培训 其他实验室的工作人员； 4）生产良好的质量控制和参考材料 可用于更广泛的科学界。经验，知识，技能和知识的财富和广度 多年来，WC-Hear赢得了声誉，包括成功参与Chear作为一个 焦油，将使HHEAR暴露的成功实施用于暴露范围 这需要严格的分析质量控制。