Treatment of dentin hypersensitivity by calcium phosphate precipitation method.

磷酸钙沉淀法治疗牙本质过敏。

基本信息

批准号：
07557271
负责人：
EBISU Shigeyuki
金额：
$ 2.3万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

We have proposed calcium phosphate precipitation (CPP) method which occluded dentin tubules with apatitic mineral for the treatment of dentin hypersensitivity. In this study, we aimed (1) to assess the feasibility of other basic solutions as the post-treatment solution for clinical use of CPP method. (2) to evaluate occluding ability of CPP method in vivo using beagle dogs. The current CPP method uses 1 mol/L NaOH (pH=12.4) as the post-treatment solution. However, its strong basicity is not desirable for clinical use. We evaluated several buffer solutions to neutralize CPP solution, then we chose 1 mol/L NaHCO_3 (pH=8.1) as the post-treatment solution. It gave almost the same occluding ability of dentinal tubules with that of NaOH.Next, we evaluated the occluding ability of dentinal tubules with CPP method using vital tooth of beagle dogs. Class V cavity was prepared in cervical of molar in beagle dogs, then the tooth was etched with 50% citric acid for 2 min to open dentinal tubules. After the application of CPP method, dentin biopsy was performed to obtain dentin specimens using cylindrical diamond point. We found that dentinal tubules were occluded homogeneously and completely with the precipitate. The depth of the precipitate was approximately 5 mum, the value was approximately half of that obtained in vitro results. The Ca/P molar ratio of the precipitate was 1.5. The value indicated that the precipitate was carbonate-apatite. We concluded that CPP method is useful for the treatment of dentin hypersensitivity since it occludes dentinal tubules homogeneously with apatitic mineral even though the depth of precipitate was shallower than that of in vitro results.

我们提出了用磷灰石矿物质封闭牙本质小管的磷酸钙沉淀（CPP）方法来治疗牙本质过敏。在本研究中，我们的目的是（1）评估其他基本溶液作为CPP方法临床使用的后处理溶液的可行性。 (2)用Beagle犬体内评价CPP方法的封堵能力。目前的CPP方法采用1 mol/L NaOH（pH=12.4）作为后处理溶液。但其碱性过强，不利于临床使用。我们评估了几种缓冲溶液来中和CPP溶液，然后选择1 mol/L NaHCO_3 (pH=8.1)作为后处理溶液。它具有与NaOH几乎相同的牙本质小管封闭能力。接下来，我们使用比格犬的活牙通过CPP法评估了牙本质小管的封闭能力。在Beagle犬磨牙颈部制备V级空洞，然后用50%柠檬酸酸蚀牙齿2 min，打开牙本质小管。应用CPP法后，利用圆柱形金刚石尖进行牙本质活检，获取牙本质标本。我们发现牙本质小管被沉淀物均匀且完全地闭塞。沉淀深度约为5μm，该值约为体外结果的一半。沉淀物的Ca/P摩尔比为1.5。该值表明沉淀物是碳酸盐-磷灰石。我们得出的结论是，CPP 方法可用于治疗牙本质过敏，因为它用磷灰石矿物质均匀地封闭牙本质小管，即使沉淀的深度比体外结果浅。