Localization of periodontal disease-associated bacteria in human periodontal pockets and its relationship to tissue destruction

牙周病相关细菌在人牙周袋中的定位及其与组织破坏的关系

基本信息

批准号：
04454472
负责人：
EBISU Shigeyuki
金额：
$ 4.35万
依托单位：
Tokushima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

This study was designed to observe the localization and distribution of certain plaque bacteria including periodontal disease-associated bacteria in human periodontal pocket. After providing informed consent, teeth and their surrounding periodontal tissue with advanced adult periodontitis were obtained carefully attempting not to change the structure of the periodontal pockets. They were processed into serial sections, and those were stained with Brown-Brenn modified Gram stain and LASB method with specific rabbit antiboeies against fifteen plaque bacterial species including periodontal disease-associated bacteria. The localization and distribution of the bacterial species in human periodontal pockets were observed under a light microscope. A small aggregates of Porphyromonas gingivalis were distributed spattering throughout the periodontal pocket. Eikenella corrodens, shich formed small clumps, was located mainly close to root surface in the middle and deep pocket zones. Fusobacterium … More nucleatum also consisted small clumps in the middle and deep pocket zones, but its localization was in the unattached plaque area. On the other hand, Camphylobacter rectus generaly formed large clumps in the middle and deep pocket zones, and located closely on both root surface and epithelial surface in periodontal pockets. Treponema denticola was observed over the unattached plaque area located mainly in the middle zone of pockets.previously, we found that E.corrodens 1073 had a cell-associated lectin-like substans (EcLS) and it functioned in the adherence of this periodontal disease-associated bacteria to various cell surface. The immunoelectron microscopic study using monoclonal antibody against EcLS revealed that EcLS was localized in the outer space of outer membrane, not in cell surface appendages such as fimbriae where many bacteria possessed adhesin. This EcLS was found to have potent mitogenic activity when cultured with B lymphocytes from BALB/c mice. EcLS stimulated murine B lymphocytes not only to proliferate, but also to differentiate into antibody-secreting cells. These findings suggest that EcLS can stimulate host cells, and does not just mediate adherence between the host cell and E.corrodens. Less

本研究旨在观察人类牙周袋中某些牙菌斑细菌（包括牙周病相关细菌）的定位和分布，在获得知情同意后，患有晚期成人牙周炎的牙齿及其周围牙周组织小心翼翼地尝试不改变所获得的结构。将它们加工成连续切片，并使用针对包括牙周病相关细菌在内的 15 种菌斑细菌的特异性兔抗体，用 Brown-Brenne 改良革兰氏染色和 LASB 方法对这些切片进行染色。光镜下观察牙周袋内细菌种类的分布，牙龈卟啉单胞菌小聚集体散布在整个牙周袋内，形成小团块，主要分布在靠近牙根面的中、深部。具核梭杆菌在中部和深处的口袋区也包含小团块，但其定位在未附着的斑块区域。另一方面，直肠弯曲杆菌一般在牙周袋的中、深部形成大的团块，并且靠近牙周袋的牙根表面和上皮表面，在主要位于牙袋中部的未附着菌斑区域观察到密螺旋体。此前，我们发现 E.corrodens 1073 具有细胞相关凝集素样物质 (EcLS)，并且它在粘附这种物质方面发挥作用。使用针对 EcLS 的单克隆抗体进行的免疫电子显微镜研究表明，EcLS 位于外膜的外部空间，而不是在许多细菌具有粘附素的细胞表面附属物中。当与 BALB/c 小鼠的 B 淋巴细胞一起培养时，EcLS 不仅能刺激小鼠 B 淋巴细胞增殖，而且能分化为细胞，具有有效的促有丝分裂活性。这些发现表明，EcLS 可以刺激宿主细胞，而不仅仅是介导宿主细胞和腐蚀杆菌之间的粘附。