Development of focal infection model result from infected root canal-associated bacteria on compromised hosts.

局灶性感染模型的开发是由受损宿主上受感染的根管相关细菌引起的。

基本信息

批准号：
12557161
负责人：
EBISU Shigeyuki
金额：
$ 7.94万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Correlation between Cyclosporin A (CsA)-induced immunosuppression and untreated or residual periapical lesions has been investigated 3 dimensionally with time, and histopathological analysis were performed. CsA treatment significantly inhibited lesion expansion.. Agter withdrawal of CsA, the lesion increased in size to a same extent in a control group. In addition, histopathological observation revealed that the difference of CsA administration volume did not affected much. However, CsA treatment induced intercellular substance in fibrous tissue to be voluminous, especially in 20mg/Kg/day administration group.In vitro, biofilm forming capacity of 7 infected root canal-associated bacterial (RCB) species to gutta-percha points (GP) was investigated morphologically. Enterococcus faecalis formed most frequently biofilm, but Fusobacterium uncleatum or Propionibacterium acnes did not form it at all. The biofilm forming capacity of 5 RCB species to extraradicular GPs on beagle dogs were evaluated by bacteriologic technique. E. faecalis identified in 71.4% of the examined samples, and was most suitable biofilm forming bacterium in extraradicular area. According to their results, compromised-animals to be administrated methylpredonisolon acetate were prepared for investigation of bacteremia by which planktonic bavteria detached from extraradicular E. faecalis biofilm caused. For 4 weeks administration, bacteremia was not induced, whereas planktonic E. faecalis cells insertion of about 10^3 times biofilm bacteria on GP in vitro in the periapical lesion became continuously bacteremia. Drug concentration and administration period were further studied, and we are continuously searching bacteremia caused by extraradicular biofilm on compromised-animal.

环孢菌素A（CSA）诱导的免疫抑制与未处理或残留的根本性病变之间的相关性已在时间上进行了3个维度，并进行了组织病理学分析。 CSA处理显着抑制了病变的扩张。CSA的戒断，病变的大小增加了对照组的程度。此外，组织病理学观察结果表明，CSA给药量的差异不大。然而，CSA处理诱导纤维组织中的细胞间物质是大量的，尤其是在20mg/kg/day给药组中。在体外，在体外，对Gutta-Percha-Percha点（GP）的体外形成7种感染根管相关的细菌（RCB）物种的生物膜能力被形成形态学。粪肠球菌形成最常见的生物膜，但叶肉芽杆菌或痤疮杆菌根本没有形成。通过细菌技术评估了5种RCB物种对Beagle狗的牙外GP的生物膜形成能力。粪肠球菌在71.4％的样品中鉴定出来，是在肌外区域形成细菌的最合适的生物膜。根据他们的结果，准备了乙酸乙酸酯的受损动物症，以研究菌血症，通过该菌血症，浮游粉与从肌外虫外虫生物膜上脱离的浮游粉红色。在4周的给药中，未诱导菌血症，而浮游生活性粪肠球菌细胞插入大约10^3倍的生物膜细菌在GP的体外，在根本性病变中成为持续的细菌血症。进一步研究了药物浓度和给药周期，我们一直在不断受损的动物膜外生物膜引起的菌血症。