A study on host-parasite interactions in human periodontal pockets.

人类牙周袋中宿主与寄生虫相互作用的研究。

基本信息

批准号：
06454541
负责人：
EBISU Shigeyuki
金额：
$ 4.1万
依托单位：
Tokushima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

We observed subgingival plaque in 18 severe adult-periodontitis affected teeth by scanning electron microscopy. In the apical one-third zone of periodontal pockets, several bacterial species aggregated with glycocalyx-like structures, which comprised net-like and mucoidal structure. In the so-called "plaque-free" zone, two types of bacterial adherence were observed : bacterial cell walls directly contacted with the root surface, and microorganisms adhered to the root surface mediated by the glycocalyx net-like materials.After Providing informed consent, 30 teeth with severe advanced adult periodontitis were extracted, and the plaque-free zones were investigated with specific antibodies against four periodontal diseaseassociated bacteria by a scanning immunoelectron microscopy method. Porphyromonas gingivalis was actually found in the plaque-free zones in four of the seven samples examined, and Treponema denticola was detected in five of nine samples. Prevotella intermedia occurred in o … More nly one of the seven samples examined, and Actinomyces viscosus was identified in four of the seven samples. More than half of the number of the spirochetes and rods detected by secondary electron imaging in the plaque-free zones did not react positively with the four specific antibodies examined by back-scattered electron imaging. This suggests that several unidentified microorganisms were present in the plaque-free zones in addition to the above four bacterial species.We also examined immunohitologically the cell densities and distribution of T and B cells in periodontitis, and analyzed their relationship in terms of periodontal tissue destruction. T cells appeared in the low density of infiltrates and increased gradually with the number of total infiltrates, whereas B cells did not appear in the small infiltrates but increased rapidly with the number of infiltrates. The number of fibroblastic cells showed negative correlation with that of the total infiltrates and B cells, but it did not correlate with the number of T cells. These findings suggest that the T cells infiltrated into the imflamed sites at the onset and increased gradually with the development of inflammation, and that B cells infiltrated behind the T cells and their number increased rapidly with inflammation. Less

我们通过扫描电子显微镜观察了18颗患有严重成人牙周炎的牙齿的龈下菌斑，在牙周袋的根尖三分之一区域中，几种细菌聚集成糖萼状结构，其中包括网状和粘液状结构。 -称为“无菌斑”区，观察到两种类型的细菌粘附：细菌细胞壁直接与根表面接触，以及微生物通过细菌细胞壁介导粘附到根表面。在知情同意后，拔除30颗患有严重晚期成人牙周炎的牙齿，通过扫描免疫电镜方法对牙菌斑无抗体区进行特异性针对四种牙周病相关细菌的检测，实际发现了牙龈卟啉单胞菌。在所检查的七个样本中，有四个样本中存在无菌斑区，在九个样本中的五个样本中检测到了中间普雷沃氏菌。七个样本中只有一个被检测到，并且在七个样本中的四个中发现了粘性放线菌，通过二次电子成像在无噬菌斑区域中检测到的螺旋体和杆状体中有一半以上没有与四个特定的反应发生阳性反应。通过背散射电子成像检查抗体，这表明除了上述四种细菌之外，在无噬菌斑区域还存在几种未鉴定的微生物。我们还通过免疫组织学检查了 T 和 B 的细胞密度和分布。牙周炎中 T 细胞出现在低密度浸润中，并随着总浸润数的增加而逐渐增加，而 B 细胞则不出现在小浸润中，但随着数量的增加而迅速增加。成纤维细胞的数量与浸润总数和 B 细胞呈负相关，但与 T 细胞的数量无关。炎症部位在发病时随炎症发展而逐渐增多，B细胞浸润在T细胞后面，且数量随炎症的增加而迅速减少。