Treatment of dentin hypersensitivity by calcium phosphate precipitation method.

磷酸钙沉淀法治疗牙本质过敏。

基本信息

批准号：
09557152
负责人：
EBISU Shigeyuki
金额：
$ 7.74万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

We have proposed calcium phosphate precipitation (CPP) method which occluded dentinal tubules with apatitic mineral for the treatment of dentin hypersensitivity. In this study, we aimed to evaluate occluding ability of CPP method in vivo using vital teeth of beagle dogs. Class V cavities were prepared in cervical of molars and canines in beagle dogs. Class V cavities were prepared in cervical of molars and canines in beagle dogs, then the teeth were etched with 50% citric acid for 2 min to open dentinal tubules. CPP solution (1M CaHPOィイD24ィエD2・2HィイD22ィエD2O, 2M HィイD23ィエD2POィイD24ィエD2) and post-treatment solution (1M NaHCOィイD23ィエD2, 0.3M NaF) were applied to the teeth with cotton swab. The teeth were divided into two groups, (1) plaque control made by brushing, and (2) nonplaque control group. Dentin biopsy was performed to obtain dentin specimens using cylindrical diamond point immediately after treatment and seven days after treatment with CPP method. The surface and fractured surface of the specimens were observed by scanning electron microscopy. We found that dentinal tubules were occluded homogeneously and completely with apatitic precipitate up to 5 μm from the dentin surface when CPP treatment was made. In non-plaque control group, the dentinal tubules became open seven days after CPP treatment. In contrast, most of the dentinal tubules were still occluded with the precipitate in plaque control group. We concluded that CPP method is useful for the treatment of dentin hypersensitivity when proper plaque control was made. In the other words, plaque control plays a key role for the duration of the dentin hypersensitivity treatment with CPP method.

我们提出了用磷灰石矿物质封闭牙本质小管的磷酸钙沉淀（CPP）方法，用于治疗牙本质过敏。在本研究中，我们旨在使用比格犬的活牙来评估 CPP 方法的体内封闭能力。在比格犬的臼齿和尖牙的颈部制备V类蛀牙，然后在比格犬的臼齿和尖牙的颈部制备V类蛀牙，然后蚀刻牙齿。用 50% 柠檬酸 2 分钟打开牙本质小管，将 CPP 溶液（1M CaHPO D24 D2、2H D22 D2O、2M HPO D23 D2PO D24 D2）和后处理溶液（1M NaHCO2D2、0.3M NaF）应用于牙齿。带棉的将牙齿分为两组，（1）通过刷牙进行牙菌斑对照，（2）在治疗后立即和治疗后7天进行牙本质活检以获取牙本质标本。通过扫描电镜观察标本表面和断面，发现牙本质小管均匀、完全地被磷灰石沉淀堵塞。在进行 CPP 治疗时，在非斑块对照组中，牙本质小管在 CPP 治疗后 7 天变得开放，相比之下，在斑块对照组中，大多数牙本质小管仍然被沉淀物堵塞。当进行适当的牙菌斑控制时，CPP 方法可用于治疗牙本质过敏。换句话说，牙菌斑控制对于牙本质的持续时间起着关键作用。过敏症治疗采用CPP法。