Monetite-Apatite Phase Transformation for an Enamel-Like Restorative Material

类牙釉质修复材料的三斜磷灰石-磷灰石相变

• 批准号: 9894790
• 负责人: Janet M. Oldak
• 金额: $ 24.75万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-03-15 至 2023-02-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9894790
• 关键词: Affect; Amalgam; Apatites; Biomechanics; Biomimetics; Ceramics; Chitosan; Crystallization; Dental; Dental Enamel; Dentin; Development; Exhibits; Future; Goals; Growth; Hardness; Human; Hydroxyapatites; In Situ; Ions; Kinetics; Longevity; Minerals; Modulus; Nanostructures; Outcome; Pathway interactions; Peptides; Phase; Polymers; Property; Research Proposals; Scheme; Structure; Surface; System; Testing; Time; Tissues; Tooth structure; alkalinity; amelogenin; base; calcium phosphate; cariogenic bacteria; chemical bond; chemical property; design; efficacy testing; experimental study; improved; inorganic phosphate; maleic acid; mechanical properties; mineralization; novel; physical property; preclinical efficacy; remineralization; restoration; restorative material; tooth surface

Project Summary / Abstract The goal of this exploratory/developmental research proposal is to explore a novel biomimetic calcium phosphate (CaP) composite material that will have an enamel-like structure and can be applied to restore posterior Class I cavities. Such an enamel-like biocomposite will integrate with the natural dental tissues through a strong chemical bond, and restore the tooth structures. It is based on the synthesis of a layered chitosan-monetite composite that can transform into an organized hydroxyapatite composite with improved mechanical properties. We demonstrated that that our newly designed amelogenin-inspired peptide P26 can promote regrowth of an enamel layer integrated with natural enamel and dentin. Building on these findings, we propose to apply the peptides to the monetite system in order to regulate the organized growth of apatite crystals at restoration-tooth interface. We hypothesize that i) organized enamel-like apatite crystals will be formed through a phase transformation of layered chitosan-monetite composite in the presence of amelogenin- inspired peptide ; and ii) the peptide will promote and control the in situ growth of organized apatite crystals at the enamel/dentin surface to integrate the biocomposite with tooth structures. We plan to test our hypotheses and approach the goal by pursuing the following three specific aims. Aim I) To synthesize a biomimetic chitosan-CaP composite with organized structure through a monetite-to-apatite phase transformation and to optimize its microstructure, biomechanical properties and setting time by incorporating an amelogenin-inspired peptide P26 and by examining the effects of additives such as Ca, phosphate, alkaline and amorphous calcium phosphate (ACP) on the abovementioned parameters. We hypothesize that incorporation of the small active peptides will assist in controlling the nanostructure of the final product and hence improve its hardness and elastic modulus. We will examine the release kinetics of mineralizing ions and P26 from the biocomposite material. Aim II) To evaluate integration of the biocomposite developed in Aim I to dental enamel and dentin using human extracted 3rd molar slabs. We will examine the interface between the biomimetic restorative material and the underlying tooth structures. We hypothesize that a strong chemical bond interface will be formed as the result of active peptide-guided remineralization at the tooth-restoration interface. If successful, this study will lead to an alternative dental restorative material to treat posterior Class I cavities, that has physical and chemical properties close to dental tissues (enamel/dentin), integrates well with the underlying tissues, and exhibits an extended longevity superior to the current restorative materials.

项目摘要 /摘要 该探索性/发展研究建议的目的是探索一种新型的仿生钙 磷酸盐（CAP）复合材料将具有牙釉质样结构，可用于恢复 后级腔。这种类似搪瓷的生物复合材料将与天然牙科组织集成 通过牢固的化学键，并恢复牙齿结构。它基于分层的合成 壳聚糖单石复合材料，可以转化为有组织的羟基磷灰石复合材料，并改善 机械性能。我们证明了我们新设计的阿米他素启发的肽P26可以 促进与天然搪瓷和牙本质集成的搪瓷层的再生。在这些发现的基础上，我们 提议将肽应用于货币系统，以调节磷灰石的有组织的生长 恢复牙齿界面处的晶体。我们假设i）有组织的牙釉质样磷灰石晶体将是 通过在氨基蛋白素的存在下通过分层壳聚糖 - 单石复合材料的相变形成 启发肽； ii）肽将促进和控制有组织的磷灰石晶体的原位生长 搪瓷/牙本质表面将生物复合材料与牙齿结构相结合。我们计划检验我们的假设 并通过追求以下三个特定目标来实现目标。目的i）合成仿生的 壳聚糖盖复合材料，通过货币至适地的相变和有组织的结构和 通过合并氨基蛋白酶启发的 肽P26并检查添加剂，例如Ca，磷酸盐，碱性和无定形钙的作用 上述参数上的磷酸盐（ACP）。我们假设掺入小型活动 肽将有助于控制最终产品的纳米结构，从而改善其硬度和 弹性模量。我们将检查矿化离子和p26的释放动力学 材料。目的ii）评估以牙齿和牙本质为目标的生物复合材料的整合 使用人类提取的第三摩尔平板。我们将检查仿生恢复之间的接口 材料和基础牙齿结构。我们假设强大的化学键界面将是 是由于在牙齿恢复界面处有活性肽引导的回忆性的结果。如果成功， 这项研究将导致一种替代性牙科修复材料来治疗后级腔，其中具有 靠近牙科组织（搪瓷/牙本质）附近的物理和化学特性，与基础良好地整合 组织，表现出延长的寿命，高于当前的修复材料。