Monetite-Apatite Phase Transformation for an Enamel-Like Restorative Material

类牙釉质修复材料的三斜磷灰石-磷灰石相变

• 批准号: 9894790
• 负责人: Janet M. Oldak
• 金额: $ 24.75万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-03-15 至 2023-02-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9894790
• 关键词: Affect; Amalgam; Apatites; Biomechanics; Biomimetics; Ceramics; Chitosan; Crystallization; Dental; Dental Enamel; Dentin; Development; Exhibits; Future; Goals; Growth; Hardness; Human; Hydroxyapatites; In Situ; Ions; Kinetics; Longevity; Minerals; Modulus; Nanostructures; Outcome; Pathway interactions; Peptides; Phase; Polymers; Property; Research Proposals; Scheme; Structure; Surface; System; Testing; Time; Tissues; Tooth structure; alkalinity; amelogenin; base; calcium phosphate; cariogenic bacteria; chemical bond; chemical property; design; efficacy testing; experimental study; improved; inorganic phosphate; maleic acid; mechanical properties; mineralization; novel; physical property; preclinical efficacy; remineralization; restoration; restorative material; tooth surface

Project Summary / Abstract The goal of this exploratory/developmental research proposal is to explore a novel biomimetic calcium phosphate (CaP) composite material that will have an enamel-like structure and can be applied to restore posterior Class I cavities. Such an enamel-like biocomposite will integrate with the natural dental tissues through a strong chemical bond, and restore the tooth structures. It is based on the synthesis of a layered chitosan-monetite composite that can transform into an organized hydroxyapatite composite with improved mechanical properties. We demonstrated that that our newly designed amelogenin-inspired peptide P26 can promote regrowth of an enamel layer integrated with natural enamel and dentin. Building on these findings, we propose to apply the peptides to the monetite system in order to regulate the organized growth of apatite crystals at restoration-tooth interface. We hypothesize that i) organized enamel-like apatite crystals will be formed through a phase transformation of layered chitosan-monetite composite in the presence of amelogenin- inspired peptide ; and ii) the peptide will promote and control the in situ growth of organized apatite crystals at the enamel/dentin surface to integrate the biocomposite with tooth structures. We plan to test our hypotheses and approach the goal by pursuing the following three specific aims. Aim I) To synthesize a biomimetic chitosan-CaP composite with organized structure through a monetite-to-apatite phase transformation and to optimize its microstructure, biomechanical properties and setting time by incorporating an amelogenin-inspired peptide P26 and by examining the effects of additives such as Ca, phosphate, alkaline and amorphous calcium phosphate (ACP) on the abovementioned parameters. We hypothesize that incorporation of the small active peptides will assist in controlling the nanostructure of the final product and hence improve its hardness and elastic modulus. We will examine the release kinetics of mineralizing ions and P26 from the biocomposite material. Aim II) To evaluate integration of the biocomposite developed in Aim I to dental enamel and dentin using human extracted 3rd molar slabs. We will examine the interface between the biomimetic restorative material and the underlying tooth structures. We hypothesize that a strong chemical bond interface will be formed as the result of active peptide-guided remineralization at the tooth-restoration interface. If successful, this study will lead to an alternative dental restorative material to treat posterior Class I cavities, that has physical and chemical properties close to dental tissues (enamel/dentin), integrates well with the underlying tissues, and exhibits an extended longevity superior to the current restorative materials.

项目概要/摘要 这项探索性/开发性研究计划的目标是探索一种新型仿生钙 磷酸盐（CaP）复合材料将具有类似牙釉质的结构，可用于修复 后 I 类空洞。这种类似牙釉质的生物复合材料将与天然牙组织融为一体 通过强大的化学键，恢复牙齿结构。它基于分层的合成 壳聚糖-三磷钙石复合材料，可以转化为有组织的羟基磷灰石复合材料，具有改进的 机械性能。我们证明，我们新设计的受牙釉蛋白启发的肽 P26 可以 促进与天然牙釉质和牙本质结合的牙釉质层的再生。基于这些发现，我们 提议将肽应用于三磷灰石系统以调节磷灰石的有组织生长 修复体-牙齿界面处的晶体。我们假设 i) 有组织的釉质状磷灰石晶体将是 在牙釉蛋白存在下，通过层状壳聚糖-三磷钙石复合材料的相变形成 激发肽； ii) 该肽将促进和控制有组织的磷灰石晶体的原位生长 牙釉质/牙本质表面，将生物复合材料与牙齿结构整合在一起。我们计划检验我们的假设 并通过追求以下三个具体目标来实现这一目标。目标 I) 合成仿生材料 壳聚糖-CaP 复合材料通过三斜磷灰石到磷灰石相变而具有有序结构，并 通过结合牙釉蛋白启发，优化其微观结构、生物力学特性和凝固时间 肽 P26 并检查添加剂（例如钙、磷酸盐、碱性钙和无定形钙）的影响 磷酸盐（ACP）对上述参数的影响。我们假设小活性的合并 肽将有助于控制最终产品的纳米结构，从而提高其硬度和 弹性模量。我们将研究生物复合材料中矿化离子和 P26 的释放动力学 材料。目标 II) 评估目标 I 中开发的生物复合材料与牙釉质和牙本质的整合 使用人类提取的第三磨牙板。我们将研究仿生修复体之间的界面 材料和下面的牙齿结构。我们假设强化学键界面将是 是由活性肽引导的牙齿修复界面再矿化的结果而形成的。如果成功的话， 这项研究将开发出一种替代牙科修复材料来治疗后 I 类蛀牙， 物理和化学特性接近牙组织（牙釉质/牙本质），与底层良好融合 组织，并且比目前的修复材料具有更长的使用寿命。