STRUCTURAL BIOLOGY OF THE ENAMEL PROTEINS

牙釉质蛋白质的结构生物学

• 批准号: 8363746
• 负责人: Janet M. Oldak
• 金额: $ 0.08万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363746
• 关键词: Apatites; Architecture; Area; Binding; Biomimetics; Dental Enamel; Development; Extracellular Matrix; Funding; Future; Gel; Goals; Grant; Growth; In Vitro; Lead; MMP-20; Mass Spectrum Analysis; Minerals; Molecular; Morphology; Nanosphere; National Center for Research Resources; Nature; Peptide Hydrolases; Principal Investigator; Proteins; Research; Research Activity; Research Infrastructure; Resources; Solutions; Source; Structure; United States National Institutes of Health; amelogenin; base; cost; octacalcium phosphate; self assembly; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The major goal of our research activities is to advance our understanding of the fundamental molecular mechanisms involved in the formation of dental enamel. We postulate that such understanding will lead to the future development of biomimetic strategies for the creation of enamel-like mineral materials. We focus on two areas: 1. The structure and function of enamel extracellular matrix components. 2) The function and mechanism of action of enamel proteinases MMP-20 and KLK-4. Our goals are: ( project #1) to identify the nature of the interactions which define protein self-assembly in solution and the nanosphere matrix architecture, at the molecular level, to define at the molecular level the ultrastructural architecture of amelogenin-based matrices formed in vitro, to characterize apatite and octacalcium phosphate crystal growth, morphology and orientation within synthetic amelogenin gel matrices in the absence and presence of the non-amelogenins. (project # 2) : To investigate the function of MMP-20 in the assembly, dis-assembly and apatite binding of amelogenin and its proteolytic products.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的首席研究员可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 我们研究活动的主要目标是增进我们对牙釉质形成所涉及的基本分子机制的理解。我们假设这种理解将导致未来用于创建牙釉质矿物材料的仿生策略的发展。我们重点研究两个领域：1.牙釉质细胞外基质成分的结构和功能。 2）牙釉质蛋白酶MMP-20和KLK-4的功能和作用机制。我们的目标是：（项目＃1）在分子水平上确定溶液中蛋白质自组装和纳米球基质结构的相互作用的性质，在分子水平上定义形成的基于牙釉蛋白的基质的超微结构在体外，表征在不存在和存在非牙釉蛋白的情况下合成牙釉蛋白凝胶基质中磷灰石和磷酸八钙晶体的生长、形态和取向。 （项目#2）：研究MMP-20在牙釉蛋白及其蛋白水解产物的组装、解组装和磷灰石结合中的功能。