STRUCTURAL BIOLOGY OF THE ENAMEL PROTEINS

牙釉质蛋白质的结构生物学

基本信息

批准号：
7601827
负责人：
Janet M. Oldak
金额：
$ 0.89万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-30 至 2008-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7601827
关键词：
Apatites Architecture Area Biomimetics Computer Retrieval of Information on Scientific Projects Database Dental Enamel Development Endopeptidases Extracellular Matrix Funding Future Gel Goals Grant Growth In Vitro Institution Lead MMP-20 Minerals Molecular Morphology Nanosphere Nature Peptide Hydrolases Proteins Recombinants Research Research Activity Research Personnel Resources Solutions Source Structure United States National Institutes of Health amelogenin base enamelin enamelysin octacalcium phosphate self assembly structural biology

Apatites Architecture Area Biomimetics Computer Retrieval of Information on Scientific Projects Database Dental Enamel Development Endopeptidases Extracellular Matrix Funding Future Gel Goals Grant Growth In Vitro Institution Lead MMP-20 Minerals Molecular Morphology Nanosphere Nature Peptide Hydrolases Proteins Recombinants Research Research Activity Research Personnel Resources Solutions Source Structure United States National Institutes of Health amelogenin base enamelin enamelysin octacalcium phosphate self assembly structural biology

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The major goal of our research activities is to advance our understanding of the fundamental molecular mechanisms involved in the formation of dental enamel. We postulate that such understanding will lead to the future development of biomimetic strategies for the creation of enamel-like mineral materials. We focus on two areas: 1. The structure and function of enamel extracellular matrix components. 2) The function and mechanism of action of enamel proteinase MMP-20 (enamelysin). Our goals are: ( project #1) to identify the nature of the interactions which define protein self-assembly in solution and the nanosphere matrix architecture, at the molecular level, to define at the molecular level the ultrastructural architecture of amelogenin-based matrices formed in vitro, to characterize apatite and octacalcium phosphate crystal growth, morphology and orientation within synthetic amelogenin gel matrices in the absence and presence of the non-amelogenins. To characterize the effects of amelogenin and non-amelogenins on apatite and octacalcium crystal growth morphology in solution. (project # 2) : To investigate the action of recombinant MMP-20 on recombinant enamel proteins amelogenin, ameloblastin and enamelin.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。我们的研究活动的主要目标是促进我们对牙齿形成涉及的基本分子机制的理解。我们假设这种理解将导致未来的仿生策略来创建类似搪瓷的矿物质材料。我们关注两个领域：1。搪瓷细胞外基质组件的结构和功能。 2）牙釉质蛋白酶MMP-20（Inamelysin）的作用和机理。我们的目标是：（项目＃1）确定在分子水平上定义蛋白质自组装的相互作用的性质，在分子水平上定义了基于阿米他素基质的超微结构基质的超微结构结构，以替代的磷灰石和层次的层次化层化层化层化的层次效果，并在分子水平上定义蛋白质水平。非淀粉蛋白的不存在和存在。为了表征蛋白质素和非淀粉蛋白酶对溶液中磷灰石和八钙晶体生长形态的影响。（项目＃2）：研究重组MMP-20对重组牙釉质蛋白氨基蛋白蛋白，蛋白蛋白和搪瓷蛋白的作用。