Studies on diagnostic methods for measurement of periodontal disease activity

牙周病活动度诊断方法的研究

基本信息

批准号：
02454435
负责人：
EBISU Shigeyuki
金额：
$ 4.1万
依托单位：
Tokushima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

This study was designed to investigate diagnostic methods for measurement of periodontal disease activity. In the first experiment, subgingival plaque and gingival crevicular fluid (GCF) were taken from 17 periodontally health sites and 53 diseased sites in 39 subjects with untreated periodontitis. Absolute counts of subgingival plaque bacteria. the proportion of Spirochetes, Motile rods, Porphyromonas gingivalis (Pg) and Prevotella intermedia (Pi), prostaglandin E_2 (PGE_2) and interleukin-1alpha (IL-1alpha) in GCF were significantly higher in diseased sites. The results suggested that these tests may aid in diagnosis of periodontal disease.In the second experiment, subgingival plaque and GCF were taken from active and inactive lesions in 10 subjects with untreated adult periodontitis and also from 7 subjects in whom periodontitis was maintained after periodontal treatment. Active diseased sites were defined as pockets showing more than 2mm loss of connective attachment during 42-day … More monitoring period. Inactive diseased sites had a pocket depth comparable to the active sites, but connective attachment level did not change during the monitoring period. Subgingival plaque bacteria, and PGE_2, IL-1alpha, IL-1beta, collagenase activity and lipopolysaccharide(LPS)in GCF we're compared in active and inactive sites.In untreated periodontitis patients, PGE_2 in GCF and absolute counts of subgingival plaque bacteria were significantly higher in active sites. IL-1alpha, IL-1beta, active collagenase in GCF and the proportion of motile rods were elevated more often in active sites. In periodontitis patients on maintenance treatment, absolute counts of bacteria were significantly higher at active sites, and PGE_2 in GCF was elevated more often at such sites.Linear discriminant analysis of data was shown to be useful for detecting active sites in untreated periodontitis, because each test alone could not distinguish them. The combination of PGE_2, IL-1alpha, IL-1beta, LPS and collagenase determinations, was most useful for detecting active sites, and the sensitivity and specificity for detection of active lesions was 62% and 100%, respectivity. In contrast, the absolute counts of bacteria could be used by itself for detection of active sites in the periodontitis patients on maintenance treatment. Its sensitivity and specificity were both 82%. In conclusion, the linear discriminant analysis could be one of the useful diagnostic methods for measurement of periodontal disease activity. Less

本研究旨在探讨测量牙周病活动度的诊断方法。在第一个实验中，对 39 名未经治疗的牙周炎受试者的 17 个牙周健康部位和 53 个患病部位采集了龈下菌斑和龈沟液 (GCF)。龈下菌斑细菌的比例。 (Pi)、GCF 中的前列腺素 E_2 (PGE_2) 和白细胞介素 1α (IL-1α) 在患病部位显着升高。结果表明这些测试可能有助于诊断牙周病。在第二个实验中，龈下菌斑和 GCF 进行了检测。取自 10 名未经治疗的成人牙周炎受试者的活动性和非活动性病变，以及 7 名在牙周治疗后仍存在牙周炎的受试者。在 42 天的监测期内，结缔附着损失超过 2 毫米，不活跃的患病部位的袋深度与活跃部位相当，但在监测期间，龈下菌斑细菌和 PGE_2 的结缔附着水平没有变化。我们比较了GCF中的IL-1α、IL-1β、胶原酶活性和脂多糖(LPS)的活性和非活性位点。在未经治疗的牙周炎患者中，在接受维持治疗的牙周炎患者中，GCF 中的 PGE_2 和龈下菌斑细菌的绝对计数在活动部位显着较高，GCF 中的活性胶原酶和活动杆的比例也更高。活性位点的细菌绝对计数明显较高，GCF 中的 PGE_2 在这些位点更常升高。数据的线性判别分析被证明可用于检测未处理的活性位点牙周炎，因为单独的每种测试都无法区分它们，PGE_2、IL-1α、IL-1β、LPS 和胶原酶测定的组合对于检测活性位点最有用，检测活动性病变的敏感性和特异性为 62%。相比之下，细菌绝对计数可以单独用于检测牙周炎患者的活性位点，其敏感性和特异性均为82%。总之，线性判别分析可能是测量牙周病活动性的有用诊断方法之一。