Bacterial Invasion of Infected Canals and Root Dentin of Human Teeth and Analysis of the Host-parasite Interaction at the Apex. - Elucidation of Pathogenesis of Refractory Cases-.

受感染的根管和人类牙齿根部牙本质的细菌入侵以及根尖处宿主-寄生虫相互作用的分析。

基本信息

批准号：
10470406
负责人：
MATSUO Takashi
金额：
$ 6.21万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

We immunologically examined the prevalence and localization of 16 selected bacteria in the infected root canal systems and dentinal tubules of the walls of the infected canals. In this study, we established the new method which could maintain the canal contents during the preparation of the sections. This method was revealed to be useful to analyze the localization of selected bacteria especially in infected root canals.All of the samples showed bacteria invasion in the root canals, however, only 70 % of the examined teeth showed bacterial invasion in the dentinal tubules. In the apical portions of the root canals, 75% of samples showed bacterial invasion. Streptococcus mutans was the most abundant bacteria detected in the Infected root canals. And S. mutans was the predominant bacteria in the coronal portions, Lactobacillus casei was predominant in the middle portions, and Streptococcus sanguis and Prevotella nigrescens/Prevotella interimedia were abundant in the apical portions of the root canals. Treponema denticola was scant in all parts of root canals. In the dentinal tubules, Fusobacterium nucleatum, Eubacterium alactolyticum, Eubacterium nodatum, L. casei, and Peptostreptococcus micros were abundant, whereas Campylobacter rectus, Actinomyces viscosus, and T. denticola were scant. Even in the canal-enlarged group, invasion of bacteria in the dentinal tubules was observed in 65% of the teeth. Analyzing the localization of the bacteria, the coronal third of the root dentin showed higher bacterial invasion than the apical third (p<0.05).This immunohistological study revealed the actual condition of the selected bacteria in infected root canal and dentin. And this study also indicated that the canal-enlargement procedure could not completely remove all the bacteria in the dentinal tubules of the roots of teeth with infected canals.

我们在免疫学上检查了感染管道壁的感染根管系统和牙本质小管中16种选定细菌的患病率和定位。在这项研究中，我们建立了一种新方法，该方法可以在制备过程中维护运河含量。该方法揭示了分析选定细菌的定位是有用的，尤其是在受感染的根管中。所有样品均显示出根管中的细菌侵袭，但是，只有70％的牙齿显示出牙本质小管中的细菌浸润。在根管的顶端部分，75％的样品显示细菌侵袭。链球菌突变是在感染根管中检测到的最丰富的细菌。链球菌和链球菌是冠状动脉部分中的主要细菌，乳杆菌在中部占主导地位，而链球菌和链球菌和普雷特拉·尼古特氏菌/prevotella互二氏菌在根管的顶端部分中很丰富。 treponema牙本质在根管的所有部分都很少。在牙本质小管中，核细菌核细菌，小杆菌，溶分裂性，肉杆菌nodatum，L。casei和peptopstopococcus micros含量丰富，而弯曲杆菌直肠，静脉内科杆菌，静脉穿刺杆菌，静脉肌cos和T. dententicola scant。即使在运河增强的组中，在65％的牙齿中也观察到牙本小管中细菌的侵袭。分析细菌的定位时，冠状牙本质的三分之一显示出比根尖的三分之一的细菌浸润（P <0.05）。该免疫组织学研究揭示了感染根管和牙本质中所选细菌的实际状况。这项研究还表明，运河增强程序无法完全清除带有感染管的牙齿根部牙本质小管中的所有细菌。