Bacterial Invasion of Infected Canals and Root Dentin of Human Teeth and Analysis of the Host-parasite Interaction at the Apex. - Elucidation of Pathogenesis of Refractory Cases-.

受感染的根管和人类牙齿根部牙本质的细菌入侵以及根尖处宿主-寄生虫相互作用的分析。

基本信息

批准号：
10470406
负责人：
MATSUO Takashi
金额：
$ 6.21万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

We immunologically examined the prevalence and localization of 16 selected bacteria in the infected root canal systems and dentinal tubules of the walls of the infected canals. In this study, we established the new method which could maintain the canal contents during the preparation of the sections. This method was revealed to be useful to analyze the localization of selected bacteria especially in infected root canals.All of the samples showed bacteria invasion in the root canals, however, only 70 % of the examined teeth showed bacterial invasion in the dentinal tubules. In the apical portions of the root canals, 75% of samples showed bacterial invasion. Streptococcus mutans was the most abundant bacteria detected in the Infected root canals. And S. mutans was the predominant bacteria in the coronal portions, Lactobacillus casei was predominant in the middle portions, and Streptococcus sanguis and Prevotella nigrescens/Prevotella interimedia were abundant in the apical portions of the root canals. Treponema denticola was scant in all parts of root canals. In the dentinal tubules, Fusobacterium nucleatum, Eubacterium alactolyticum, Eubacterium nodatum, L. casei, and Peptostreptococcus micros were abundant, whereas Campylobacter rectus, Actinomyces viscosus, and T. denticola were scant. Even in the canal-enlarged group, invasion of bacteria in the dentinal tubules was observed in 65% of the teeth. Analyzing the localization of the bacteria, the coronal third of the root dentin showed higher bacterial invasion than the apical third (p<0.05).This immunohistological study revealed the actual condition of the selected bacteria in infected root canal and dentin. And this study also indicated that the canal-enlargement procedure could not completely remove all the bacteria in the dentinal tubules of the roots of teeth with infected canals.

我们通过免疫学方法检查了受感染根管系统和受感染根管壁牙本质小管中 16 种选定细菌的流行情况和定位。在本研究中，我们建立了在切片制备过程中保持根管内容物的新方法。该方法被证明可用于分析选定细菌的定位，特别是在受感染的根管中。所有样本均显示细菌侵入根管，然而，只有 70% 的检查牙齿显示细菌侵入牙本质小管。在根管根尖部分，75%的样本显示有细菌侵入。变形链球菌是在受感染的根管中检测到的最丰富的细菌。冠部以变形链球菌为主，中部以干酪乳杆菌为主，根管顶端部以血链球菌和黑普氏菌/中间普氏菌为主。齿根螺旋体在根管各部位均很少见。牙本质小管中，具核梭杆菌、解乳真杆菌、结节真杆菌、干酪乳杆菌和微小消化链球菌含量丰富，而直肠弯曲杆菌、粘性放线菌和齿垢毛细管菌含量很少。即使在根管扩大组中，也有 65% 的牙齿观察到细菌侵入牙本质小管。分析细菌的定位，牙根牙本质冠部三分之一的细菌侵袭率高于根尖部三分之一（p<0.05）。这项免疫组织学研究揭示了受感染根管和牙本质中所选细菌的实际状况。这项研究还表明，根管扩大手术并不能完全清除根管感染的牙齿根部牙本质小管中的所有细菌。