A defense mechanism of a living body in the odontoblastic complex via gap junction

成牙本质细胞复合体中活体通过间隙连接的防御机制

基本信息

批准号：
14370614
负责人：
IKEDA Hideharu
金额：
$ 5.5万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

In our series of studies, we electrophysiologically obtained the function of odontoblasts and a direct, non-neural transduction mechanism between odontoblasts and between odontoblasts and neighboring cells. We recognized dentinal tubules as that have a close relation with odontoblasts as the pathway for several stimuli, and focused on the defense mechanism of outward flow of dentinal fluid, microcirculation of dental pulp and chronic dental pain.We revealed1.Dye coupling using low molecule dye2.Rack of evidence that there were voltage dependent currents in feline odontoblasts3.Defense mechanism of the outward flow against the LPS entry through dentinal tubules of intact and caries affected dentin4.Evidence of a functional syncytium of odontoblast complex5.Effect of aging on electrical conductance between odontoblasts6.Effect of extracellular pH on electrical conductance between odontoblasts7.Effect of mechanical distortion of odontoblast cell membrane electrical conductance between odontoblast8.Application of whole-cell voltage-clamp method to feline odontoblast9.Gap junction between human odontoblasts and neighboring cells10.Effect of iontophoresis and pulpward hydrostatic pressure on drug delivery through dentinal tubules 111.Effect of pulpal components on dentin permeability12.Changes in human sensation and neural activity with aging13.Neuropathic orofacial pain evoked by cold stimulus of oral mucosa14.Microcirculation in the dental pulp

在我们的系列研究中，我们从电生理学角度获得了成牙本质细胞的功能以及成牙本质细胞之间以及成牙本质细胞与邻近细胞之间的直接的、非神经的转导机制。我们认识到与成牙本质细胞关系密切的牙本质小管是多种刺激的通路，并重点研究了牙本质液外流、牙髓微循环和慢性牙痛的防御机制。我们揭示了1.使用低分子染料进行染料偶联2 .大量证据表明猫成牙本质细胞中存在电压依赖性电流3.针对完整和龋齿受影响的牙本质小管的LPS进入的向外流动的防御机制牙本质4.成牙本质细胞复合体功能性合胞体的证据5.衰老对成牙本质细胞间电导的影响6.细胞外pH值对成牙本质细胞间电导的影响7.成牙本质细胞膜机械变形对成牙本质细胞间电导的影响8.全细胞电压的应用-猫成牙本质细胞的钳夹法9.人类成牙本质细胞与邻近细胞之间的间隙连接细胞10.离子电渗疗法和牙髓静水压对牙本质小管药物输送的影响111.牙髓成分对牙本质渗透性的影响12.人体感觉和神经活动随衰老的变化13.口腔粘膜冷刺激引起的神经性口面部疼痛14.牙齿微循环纸浆