Electorophysiological and biochemistrical study on protecture mechaninism of odontoblasts identified with specific monoclonal antibodies
特异性单克隆抗体对成牙本质细胞保护机制的电生理生化研究
基本信息
- 批准号:10470404
- 负责人:
- 金额:$ 8.58万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1998
- 资助国家:日本
- 起止时间:1998 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We showed the functional link between odontoblasts (OB) and other type of pulpal cells (PC) beneath the odontoblastic layer.1. Isolation and in vitro immunization : From cat and human dental pulp, outer pulpal cells including odontoblasts were freshly isolated. After fusion and screening, monoclonal antibodies that labeled odontoblasts were chosen.2. Patch-clamp technique : Cells having a monopolar process and an oval cell body were used for patch-clamp recording. We succeeded to record single-channel activity and then nystatin-perforated whole-cell configuration was performed. Simply linear relation between current and voltage was obtained.3. Dye-coupling : When lucifer yellow was injected into one of two types of cells with iontophoresis, the other type of cell was also labeled. But uncoupled cells, even though they were close to the cells, were not labeled, suggesting that lucifer yellow did not leak to the outer-cellular space and labeled the cell membrane, but spread into different kind of cells through dye-coupling.4. Gap-junction : EM study showed that the couplings mentioned above were gap-junctions. After we confirmed that lucifer yellow injected in an OB or a PC transferred to the other type of cell(s), cells were fixed and used for EM study. Between two types of cells, gap-junctions were always observed.5. Electrophysiological evidence of electrical coupling : Current could pass bidirectionally through the structure confirmed above morphologically. However, asymmetric transduction was observed.These results demonstrate that OBs and PCs are functionally linked and work as a network and that OBs work as a sensor of external stimuli.
我们展示了成牙本质细胞(OB)和成牙本质细胞层下方其他类型的牙髓细胞(PC)之间的功能联系。1.分离和体外免疫:从猫和人的牙髓中新鲜分离包括成牙本质细胞的外牙髓细胞。经过融合筛选,筛选出标记成牙本质细胞的单克隆抗体。 2.膜片钳技术:具有单极突起和椭圆形细胞体的细胞用于膜片钳记录。我们成功记录了单通道活动,然后进行了制霉菌素穿孔的全细胞配置。得到了电流与电压的简单线性关系。 3.染料偶联:当通过离子电渗疗法将荧光黄注射到两种类型的细胞中的一种时,另一种类型的细胞也被标记。而未偶联的细胞,即使距离细胞很近,也没有被标记,这表明荧光黄并没有泄漏到细胞外间隙并标记细胞膜,而是通过染料偶联扩散到不同种类的细胞中。4.间隙连接:电镜研究表明上述耦合是间隙连接。在我们确认注射到 OB 或 PC 中的荧光黄转移到其他类型的细胞后,细胞被固定并用于 EM 研究。两种细胞之间总是存在间隙连接。5.电耦合的电生理学证据:电流可以双向通过上述形态学证实的结构。然而,观察到不对称转导。这些结果表明 OB 和 PC 在功能上相连并作为网络工作,并且 OB 作为外部刺激的传感器。
项目成果
期刊论文数量(86)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
H. Ikeda, H. Suda: "Ageing influences objective neural activity and subjective pulpal sensation"Archs. Oral Biol.. (in press). (2000)
H. Ikeda、H. Suda:“衰老影响客观神经活动和主观牙髓感觉”Archs。
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- 影响因子:0
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- 通讯作者:
Ikeda H, Suda H.: "Phantom tooth pain triggered by cold stimulation"Pain Research. (in press).
Ikeda H、Suda H.:“冷刺激引发的幻牙痛”疼痛研究。
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- 影响因子:0
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H.Ikeda et al.: "Dye-coupling between odontoblasts and other pulpal cells" J.dent Res. 77(Spec.Issue). 335 (1998)
H.Ikeda 等人:“成牙本质细胞和其他牙髓细胞之间的染料偶联”J.dent Res。
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- 影响因子:0
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H. Ikeda, H. Suda: "Lucifer Yellow can pass through dye-coupling between odontoblasts and other pulpal cells"Archs. Oral Biol. (in press). (2000)
H. Ikeda、H. Suda:“荧光黄可以通过成牙本质细胞和其他牙髓细胞之间的染料偶联”Archs。
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- 影响因子:0
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H. Ikeda et al.: "Neurobiology of Mastication-from Molecular to Systems Approach"Elsevier Science B.V.. 525 (1999)
H. Ikeda 等人:“咀嚼神经生物学 - 从分子到系统方法”Elsevier Science B.V.. 525 (1999)
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IKEDA Hideharu其他文献
IKEDA Hideharu的其他文献
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{{ truncateString('IKEDA Hideharu', 18)}}的其他基金
Reclassification of pulpal sensations uisng functional Magnetic Resonance Imaging (fMRI) and analysis of Transient Receptor Potential (TRP) channel receptor ptoteins
使用功能性磁共振成像 (fMRI) 重新分类牙髓感觉并分析瞬时受体电位 (TRP) 通道受体蛋白
- 批准号:
24659845 - 财政年份:2012
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
A study on pulp lymphatic system using multiphoton-excitation microscopy and analysis of membrane potential-sensitivity
牙髓淋巴系统的多光子激发显微镜研究及膜电位敏感性分析
- 批准号:
22659344 - 财政年份:2010
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Interaction between odontoblastic syncytium coupled through gap-junction and pulpal nervous and circulatory system
通过间隙连接耦合的成牙本质细胞合胞体与牙髓神经和循环系统之间的相互作用
- 批准号:
20390482 - 财政年份:2008
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
A defense mechanism of a living body in the odontoblastic complex via gap junction
成牙本质细胞复合体中活体通过间隙连接的防御机制
- 批准号:
14370614 - 财政年份:2002
- 资助金额:
$ 8.58万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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