Bioinorganic Chemistry of Catechols: Siderophores, Adhesive Proteins and Biomimetic Analogs

儿茶酚的生物无机化学：铁载体、粘附蛋白和仿生类似物

基本信息

批准号：
1710761
负责人：
Alison Butler
金额：
$ 48万
依托单位：
University of California-Santa Barbara
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-01 至 2021-10-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1710761&HistoricalAwards=false
关键词：
Bioinorganic Chemistry Catechols Siderophores Adhesive

项目摘要

With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Professor Alison Butler from the University of California, Santa Barbara to investigate the properties of compounds called catechol-siderophores that are produced by bacteria. The compounds are similar to the molecules that make it possible for mussels to cling tenaciously to rocks in the ocean. This research examines the reasons for the recently discovered properties that allow mussels to cling even to slick, wet surfaces. This project may provide an understanding of the biological phenomenon and may lead to the design of compounds that can adhere even under water. The role of catechol-siderophores in iron acquisition by bacteria is also investigated. This research project provides broad multidisciplinary training for graduate and undergraduate students. Professor Butler and her students are involved in outreach workshops including, CAMP (California Alliance for Minority Participation), an NSF-funded program supporting minority undergraduate students in science, technology, engineering and mathematics fields, and SCOPE (Science as a Career Outreach Project Experiment), a program for graduate students to talk in local high schools about undergraduate and graduate education and the value of the sciences as a career choice.The research is investigates siderophores and analogs containing both catechol and amine functional groups that mimic the strong wet adhesive properties of mussel foot proteins. The overall goals of the first part of this proposal are to elucidate the synergistic role of the catechol and the amine functionalities in displacing the interfacial hydration layer that develops on mineral surfaces leading to robust adhesion. The approach is to design and investigate the surface adhesive properties of new biomimetic compounds through direct force measurements with a surface forces apparatus. The goals of the second part of this research are to investigate the biosynthesis, uptake, and iron(III) release mechanisms of acylated tetra L-serine, and tris-catechol siderophores through investigation of key fatty acid coenzyme A ligases, outer membrane receptor proteins, and esterases identified in the genomes of the producing bacteria.

有了这个奖项，化学生命过程的化学过程计划是为加利福尼亚大学圣塔芭芭拉分校的艾莉森·巴特勒教授提供资金，以调查细菌生产的称为Catechol-Sindidophores的化合物的特性。这些化合物类似于使贻贝顽强地固定在海洋中的岩石的分子。这项研究研究了最近发现的特性的原因，这些特性使贻贝甚至可以紧贴湿润的表面。该项目可能会提供对生物学现象的理解，并可能导致甚至在水下粘附的化合物的设计。还研究了儿茶酚 - 辅助载体在细菌获取铁中的作用。该研究项目为研究生和本科生提供了广泛的多学科培训。巴特勒教授和她的学生参与了外展研讨会，包括CAMP（加利福尼亚州少数派参与联盟），NSF资助的计划，为科学，技术，工程和数学领域的少数派本科生提供支持（科学作为职业外展项目实验）），一个供研究生在当地高中谈论本科和研究生教育以及科学作为职业选择的价值的计划。研究是对辅助型和胺功能组的辅助载体和类似物的研究，这些功能群体模仿了强大的湿粘合剂。贻贝脚蛋白。该提案第一部分的总体目标是阐明儿茶酚和胺功能在置换界面上发展的界面水合层中的协同作用，从而在矿物表面上发展，从而导致可靠的粘附。该方法是通过表面力设备的直接力测量来设计和研究新仿生化合物的表面粘合性能。这项研究的第二部分的目标是通过研究通过研究钥匙脂肪酸辅酶A连接酶，外膜受体蛋白质的蛋白，在产生细菌的基因组中鉴定出来的酯酶。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Precursor-directed biosynthesis of catechol compounds in Acinetobacter bouvetii DSM 14964

布韦不动杆菌中儿茶酚化合物的前体定向生物合成 DSM 14964

DOI：
10.1039/d0cc04171h
发表时间：
2020
期刊：
Chemical Communications
影响因子：
4.9
作者：
Reitz, Zachary L.;Butler, Alison
通讯作者：
Butler, Alison

Ruckerbactin Produced by Yersinia ruckeri YRB Is a Diastereomer of the Siderophore Trivanchrobactin Produced by Vibrio campbellii DS40M4

由鲁克氏耶尔森菌 YRB 产生的 Ruckerbactin 是由坎贝氏弧菌 DS40M4 产生的铁载体 Trivanchrobactin 的非对映异构体

DOI：
10.1021/acs.jnatprod.1c01047
发表时间：
2021
期刊：
Journal of Natural Products
影响因子：
5.1
作者：
Thomsen, Emil;Reitz, Zachary L.;Stow, Parker R.;Dulaney, Kalana;Butler, Alison
通讯作者：
Butler, Alison

Genomic analysis of siderophore β-hydroxylases reveals divergent stereocontrol and expands the condensation domain family

DOI：
10.1073/pnas.1903161116
发表时间：
2019-10-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Reitz, Zachary L.;Hardy, Clifford D.;Butler, Alison
通讯作者：
Butler, Alison

A suite of asymmetric citrate siderophores isolated from a marine Shewanella species