Mechanistic Investigations of Vanadium Haloperoxidases

钒卤过氧化物酶的机理研究

基本信息

批准号：
0719553
负责人：
Alison Butler
金额：
$ 45.5万
依托单位：
University of California-Santa Barbara
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-15 至 2012-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0719553&HistoricalAwards=false
关键词：
Mechanistic Investigations Vanadium Haloperoxidases

项目摘要

This award in the Inorganic, Bioinorganic and Organometallic Chemistry program supports work by Professor Alison Butler at the University of California at Santa Barbara to probe reactions catalyzed by vanadium bromoperoxidases (V-BrPO) from species of marine red algae in the biosynthesis of brominated spiro--bicyclic sesquiterpene natural products, brominated fused-ring diterpene natural products, brominated acyclic monoterpene natural products, and bromofuranone natural products. The mechanism of action of these enzymes will be further investigated through bromine isotope fractionation studies. Many marine natural products and enzymes have important industrial applications in the specialty chemical, diagnostics, pharmaceutical and biotechnology industries. Mechanistic enzyme studies of V-BrPO can be used to form the basis of an economical, alternative and environmentally sound source of brominated marine natural products, as well as further elucidating the mechanism of halogenation. Elucidation of pathways for the biosynthesis of new compounds of biomedical and industrial importance is an anticipated impact. This project supports the education and training of graduate students, undergraduate students and postdoctoral fellows in a highly interdisciplinary research project in a manner that will serve to broaden and deepen the level of research training. The graduate students and PI participate in an extensive UCSB campus network providing undergraduate research training and K-12 outreach, across diverse populations.

该奖项在无机，生物促和有机金属化学计划中支持加利福尼亚大学圣塔芭芭拉分校的艾莉森·巴特勒教授的工作，以探测由钒溴过氧化物酶（V-BRPO）催化的反应，该反应是从海洋红色藻类物种中的生物合成中的生物合成的物种。 -bicyclic倍半萜天然产品，溴化融合的二萜天然产品，溴化无环单丙烯天然产品和溴呋喃酮天然产品。这些酶的作用机理将通过溴同位素分馏研究进一步研究。许多海洋天然产品和酶在特种化学，诊断，药物和生物技术行业中具有重要的工业应用。 V-BRPO的机械酶研究可用于构成溴化海洋天然产物的经济，替代和环境声音来源的基础，并进一步阐明卤素化的机制。阐明生物医学和工业重要性新化合物的生物合成途径是一种预期的影响。该项目支持高度跨学科研究项目的研究生，本科生和博士后研究员的教育和培训，以扩大和加深研究培训水平。研究生和PI参加了一个广泛的UCSB校园网络，该网络在各种人群中提供了本科研究培训和K-12外展。