Amphiphilic Siderophores: Biosynthesis, Iron Uptake and Effect on Microbial Growth

两亲性铁载体：生物合成、铁吸收及其对微生物生长的影响

基本信息

批准号：
1411941
负责人：
Alison Butler
金额：
$ 47.1万
依托单位：
University of California-Santa Barbara
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-01 至 2018-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1411941&HistoricalAwards=false
关键词：
Amphiphilic Siderophores Biosynthesis Iron Uptake

项目摘要

Nearly all bacteria require iron to grow. Understanding the process by which bacteria obtain iron from their surroundings provides valuable insight into how to disrupt the iron uptake process, and suggests potential new strategies to limit growth of deleterious microbes. The focus of this proposal is to find out how unique compounds called siderophores are produced for iron uptake in Vibrio harveyi and other microbes for which their production is tied to bacterial colony formation and survival. Siderophores are natural materials produced by bacteria that bind iron(III) very tightly playing a vital role in iron uptake by the bacterium. Through these investigations, graduate students will be trained in the application of a broad spectrum of modern techniques to a multidisciplinary research program. The graduate students will, in turn, also gain valuable experience as mentors to undergraduate researchers in the laboratory, and undergraduates will gain hands-on research experience.With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Alison Butler from the University of California, Santa Barbara to investigate the biosynthesis of key amphiphilic siderophores, such as the amphi-enterobactins, to investigate their physical properties including the ferric stability constants and membrane partitioning constants for different forms of the amphi-enterobactins, to investigate key esterases and outer membrane receptor proteins present in the genome of V. harveyi which are suspected to be important in the mechanism of iron uptake, as well as to investigate other key amphiphilic siderophores. V. harveyi is the widely studied model organism for quorum sensing, the process by which bacteria sense cell density. Discoveries from these investigations will provide new insights into the importance of the fatty acid in acylated siderophores in the iron uptake process.

几乎所有细菌都需要铁才能生长。了解细菌从周围环境获取铁的过程可以为了解如何破坏铁的吸收过程提供有价值的见解，并提出限制有害微生物生长的潜在新策略。该提案的重点是找出哈维弧菌和其他微生物中如何产生一种称为铁载体的独特化合物来吸收铁，这些微生物的产生与细菌菌落的形成和存活有关。铁载体是细菌产生的天然物质，它与铁 (III) 紧密结合，在细菌吸收铁的过程中发挥着至关重要的作用。通过这些调查，研究生将接受将广泛的现代技术应用于多学科研究项目的培训。反过来，研究生也将获得作为实验室本科生研究人员导师的宝贵经验，而本科生也将获得实践研究经验。通过该奖项，化学系的生命过程化学项目将资助艾莉森·巴特勒加州大学圣塔芭芭拉分校研究关键两亲性铁载体（例如两性肠杆菌素）的生物合成，以研究它们的物理特性，包括不同形式的铁稳定常数和膜分配常数两性肠杆菌素，研究 V. harveyi 基因组中存在的关键酯酶和外膜受体蛋白，这些蛋白被怀疑在铁吸收机制中很重要，并研究其他关键的两性铁载体。 V. harveyi 是广泛研究的群体感应模式生物，群体感应是细菌感知细胞密度的过程。这些研究的发现将为酰化铁载体中脂肪酸在铁吸收过程中的重要性提供新的见解。