Amphiphilic Siderophores: Biosynthesis, Iron Uptake and Effect on Microbial Growth

两亲性铁载体：生物合成、铁吸收及其对微生物生长的影响

基本信息

批准号：
1411941
负责人：
Alison Butler
金额：
$ 47.1万
依托单位：
University of California-Santa Barbara
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-01 至 2018-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1411941&HistoricalAwards=false
关键词：
Amphiphilic Siderophores Biosynthesis Iron Uptake

项目摘要

Nearly all bacteria require iron to grow. Understanding the process by which bacteria obtain iron from their surroundings provides valuable insight into how to disrupt the iron uptake process, and suggests potential new strategies to limit growth of deleterious microbes. The focus of this proposal is to find out how unique compounds called siderophores are produced for iron uptake in Vibrio harveyi and other microbes for which their production is tied to bacterial colony formation and survival. Siderophores are natural materials produced by bacteria that bind iron(III) very tightly playing a vital role in iron uptake by the bacterium. Through these investigations, graduate students will be trained in the application of a broad spectrum of modern techniques to a multidisciplinary research program. The graduate students will, in turn, also gain valuable experience as mentors to undergraduate researchers in the laboratory, and undergraduates will gain hands-on research experience.With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Alison Butler from the University of California, Santa Barbara to investigate the biosynthesis of key amphiphilic siderophores, such as the amphi-enterobactins, to investigate their physical properties including the ferric stability constants and membrane partitioning constants for different forms of the amphi-enterobactins, to investigate key esterases and outer membrane receptor proteins present in the genome of V. harveyi which are suspected to be important in the mechanism of iron uptake, as well as to investigate other key amphiphilic siderophores. V. harveyi is the widely studied model organism for quorum sensing, the process by which bacteria sense cell density. Discoveries from these investigations will provide new insights into the importance of the fatty acid in acylated siderophores in the iron uptake process.

几乎所有细菌都需要铁生长。了解细菌从其周围环境中获得铁的过程为如何破坏铁吸收过程提供了宝贵的见解，并提出了潜在的新策略来限制有害微生物的生长。该提案的重点是找出如何生产称为铁载体的独特化合物，以在颤音和其他微生物中产生与细菌菌落形成和生存相关的微生物中的铁。铁载体是由细菌产生的天然材料，结合铁（III）非常紧密地在细菌的铁吸收中起着至关重要的作用。通过这些调查，将对研究生进行多种现代技术的应用培训。 The graduate students will, in turn, also gain valuable experience as mentors to undergraduate researchers in the laboratory, and undergraduates will gain hands-on research experience.With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Alison Butler from the University of California, Santa Barbara to investigate the biosynthesis of key amphiphilic siderophores, such as the amphi-enterobactins, to investigate their physical包括稳定性常数和不同形式的两栖动物的膜分配常数，以研究V. harveyi基因组中存在的关键酯酶和外膜受体蛋白，这些蛋白质涉嫌在铁的机理中很重要，并研究了其他关键的重要性分支机构。 V. harveyi是广泛研究的用于法定感应的模型生物，这是细菌感知细胞密度的过程。从这些研究中发现的发现将为铁酸在铁摄取过程中脂肪酸的重要性提供新的见解。