Metalloenzyme Catalyzed Halogenations

金属酶催化卤化反应

• 批准号: 8716229
• 负责人: Alison Butler
• 金额: $ 24.35万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-01-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8716229&HistoricalAwards=false
• 关键词: Metalloenzyme; Catalyzed; Halogenations

The goal of this award is to elucidate the reactivities and mechanisms of two newly discovered haloperoxidase metalloenzymes, a vanadium bromoperoxidase (V-BrPO) and a non-heme iron (III) bromoperoxidase (FeNH-BrPO) which are both isolated from marine algae. V-BrPO and FeNH-BrPO are the first non-heme haloperoxidases to be discovered. Experiments are planned to test whether each metal ion functions as a Lewis acid catalyst or as an electron transfer catalyst, shuttling between different oxidation states. Both V-BrPO and FeNH-BrPO catalyze the bromination of pyrimidine- containing nucleic acids, cytosine and uracil. The reactivities of the two enzymes will be studied in terms of a) the types of organic compounds that can be brominated, b) the catalase and superoxide dismutase activity of the enzymes and c) the mechanism of reoxidation of the reduced derivatives of the enzymes. Halogenated organic compounds occur widely in marine organisms. Some of them have exciting biological activity. They represent a valuable, but neglected natural resource. The proposed research will help us understand how the myriad halogenated compounds are formed.

该奖项的目的是阐明两种新发现的卤代氧化酶金属酶的反应性和机制，一种钒溴氯氧化酶（V-BRPO）和一个非黄蜂铁（III）溴辣氧化酶（FENH-BRPO），它们都与海洋藻类分离。 V-BRPO和FENH-BRPO是第一个被发现的非血红素卤素氧化酶。 计划测试每种金属离子是刘易斯酸催化剂还是电子转移催化剂，在不同的氧化状态之间穿梭。 V-BRPO和Fenh-Brpo都催化了氨基胺含有核酸，胞嘧啶和尿嘧啶的溴化。 将根据a）可以溴化的有机化合物的类型，b）酶的过氧化氢酶和超氧化物歧化酶活性以及c）酶还原衍生物的再氧化机制。 卤代有机化合物在海洋生物中广泛发生。 他们中的一些人具有令人兴奋的生物学活动。 它们代表了有价值但被忽视的自然资源。 拟议的研究将有助于我们了解如何形成无数卤化化合物。