Biomimetic and Enzyme Investigations of Haloperoxides

卤过氧化物的仿生和酶研究

基本信息

批准号：
9529374
负责人：
Alison Butler
金额：
$ 33万
依托单位：
University of California-Santa Barbara
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1996
资助国家：
美国
起止时间：
1996-08-01 至 2001-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9529374&HistoricalAwards=false
关键词：
Biomimetic Enzyme Investigations Haloperoxides

项目摘要

This award in the Inorganic, Bioinorganic, and Organometallic Chemistry program supports research by Dr. Alison Butler of the Chemistry Department, University of California at Santa Barbara, on marine haloperoxidase enzymes. The reactivities of vanadium bromoperoxidase and FeHeme bromoperoxidase, including their roles in biosynthesis, will be investigated using functional model complexes and enzyme studies. Vanadium complexes of alpha-helical peptides and alpha-helical peptide bundles that closely resemble vanadium haloperoxidases will be synthesized and tested. In addition some new mesoporous and titanium dioxide materials will be prepared and tested for peroxidative halogenation reactivity. Reactivity comparisons will be made to marine haloperoxides isolated from chiral halogenated acetogenins and sesquiterpenes produced from marine algae. The halide selectivity of vanadium haloperoxidases from algae that evolve chlorinated and brominated hydrocarbons will be investigated. Many marine natural products and enzymes have important industrial applications in the specialty chemical, diagnostics, pharmaceutical, and biotechnology industries. Development of the use of some of these natural products has been inhibited by lack of availability of sufficient quantities. This work will identify functional mimics for some marine haloperoxidases present in marine algae. These compounds have potential use as catalysts for production of oxidants and halogenating agents in situ, processes that would be environmentally beneficial alternatives to present technology.

该无机、生物无机和有机金属化学项目奖项支持加州大学圣巴巴拉分校化学系的 Alison Butler 博士关于海洋卤过氧化物酶的研究。将使用功能模型复合物和酶研究来研究钒溴过氧化物酶和 FeHeme 溴过氧化物酶的反应性，包括它们在生物合成中的作用。将合成和测试与钒卤过氧化物酶非常相似的α螺旋肽和α螺旋肽束的钒复合物。此外，还将制备一些新的介孔和二氧化钛材料并测试其过氧化卤化反应活性。将与从海藻产生的手性卤代乙酰苷和倍半萜中分离的海洋卤过氧化物进行反应性比较。将研究来自产生氯化和溴化碳氢化合物的藻类的钒卤过氧化物酶的卤化物选择性。许多海洋天然产物和酶在特种化学品、诊断、制药和生物技术行业具有重要的工业应用。由于缺乏足够的数量，这些天然产品的使用发展受到抑制。这项工作将鉴定海洋藻类中存在的一些海洋卤过氧化物酶的功能模拟物。这些化合物具有用作原位生产氧化剂和卤化剂的催化剂的潜在用途，该过程将是现有技术的环境有益的替代方案。