DNA Sequencing with Nanopores and Transverse Tunneling

利用纳米孔和横向隧道进行 DNA 测序

• 批准号: 1102230
• 负责人: Brian Willis
• 金额: $ 36万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2015-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1102230&HistoricalAwards=false
• 关键词: DNA; Sequencing; Nanopores; Transverse; Tunneling

The objective of this program is to investigate nanoelectrode nanopore DNA sequencing devices combined with a hybrid approach that measures the electrical response of the devices to short hybridized DNA sequences. The intellectual merit is to investigate a new approach to DNA sequencing that has a strong theoretical basis but needs to be developed experimentally. The proposed research will test well-developed hypotheses that DNA can be sequenced by transverse tunneling measurements. The first hypothesis is that transverse tunneling is sensitive to specific nucleotides and that sequences of nucleotides can be detected in nanoelectrode tunneling devices. The second hypothesis relaxes the need for single base resolution by using short probes that hybridize with DNA. These labeled strands are proposed to give rise to significant fluctuations of the tunneling current in nanopore devices. The broader impacts are to discover a new type of low cost DNA sequencing device that enables new medical applications for the benefit of the general public. The successful discovery of low cost nanodevices for DNA sequencing would be transformative for personalized genomics by greatly expanding our ability to study, diagnose, and treat disease. A successful outcome of the research will have a tremendous impact on healthcare world-wide by facilitating wide-spread DNA sequencing technology. The research will also foster outreach to the general public to disseminate information about the potential benefits and ethical risks of genome sequencing technology. Other broader impacts include impacts on graduate and undergraduate education and summer outreach activities to high school students, including underrepresented groups.

该程序的目的是研究纳米电极纳米孔DNA测序设备，结合了杂化方法，该方法测量了设备对短杂交DNA序列的电响应。 智力优点是研究一种新的DNA测序方法，该方法具有强大的理论基础，但需要在实验中开发。 拟议的研究将检验出良好的假设，即可以通过横向隧道测量来对DNA进行测序。 第一个假设是横向隧道对特定的核苷酸敏感，并且可以在纳米电极隧道装置中检测到核苷酸的序列。 第二个假设通过使用与DNA杂交的短探针来放松单碱基分辨率。 提出这些标记的链，以引起纳米孔设备中隧道电流的显着波动。 更广泛的影响是发现一种新型的低成本DNA测序设备，该设备可实现新的医疗应用，以使公众受益。 通过大大扩展我们的研究，诊断和治疗疾病的能力，成功发现用于DNA测序的低成本纳米台词将是个性化基因组学的变化。 这项研究的成功结果将通过促进广泛的DNA测序技术对全世界的医疗保健产生巨大影响。 这项研究还将促进与公众的宣传，以传播有关基因组测序技术的潜在收益和道德风险的信息。 其他更广泛的影响包括对包括代表性不足的团体在内的高中生对研究生和本科教育以及夏季外展活动的影响。