Cell and Tissue Imaging

细胞和组织成像

基本信息

批准号：
10583247
负责人：
James Alexander Fitzpatrick
金额：
$ 10.37万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1996
资助国家：
美国
起止时间：
1996-12-01 至 2028-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10583247
关键词：
3-Dimensional Acceleration Animals Area Basic Science Behavior Biological Assay Cell physiology Cells Charge Cilia Consultations Contrast Media Cryoelectron Microscopy Custom Data Analyses Data Set Dedications Development Diabetes Mellitus Dimensions Education Educational workshop Electron Microscopy Electrons Ensure Equipment Experimental Designs Faculty Feedback Fluorescence Funding Generations Goals Grant Heart Human Resources Image Image Analysis Imaging Techniques Imaging technology Immunohistochemistry Individual Informatics Institution Investments Ions Laboratories Laboratory Research Learning Light Liver Macromolecular Complexes Metabolic Diseases Methodology Micro Electron Diffraction Microscope Microscopic Microscopy Morphology Optics Pathogenesis Physiological Play Preparation Process Property Proteins Publications Research Research Personnel Resolution Roentgen Rays Role Sampling Schedule Scholarship Services Specialist Specimen Speed Structure System Technical Expertise Techniques Technology Time Tissue imaging Tissues Training Translational Research Universities Visualization Washington Work X ray microscopy biological research cellular imaging cost data acquisition imaging facilities improved innovation instrumentation islet light microscopy live cell imaging member microscopic imaging molecular imaging new technology operation polypeptide professor programs quantitative imaging research study response single molecule small molecule superresolution imaging superresolution microscopy technology platform tissue fixing tool translational study treatment strategy two-dimensional two-photon

项目摘要

PROJECT SUMMARY: Cell and Tissue Imaging Core Advanced cellular microscopy is a powerful tool for biological research and plays an important role in the study of the pathogenesis and eventual treatment of diabetes mellitus. Imaging technology has evolved rapidly over the last decade leading to improvements in resolution, sensitivity and speed that have created new opportunities for studying processes across many orders of magnitude and in real-time in living cells and animals. However, the costs of increasingly sophisticated equipment are substantial and the expertise to efficiently use, maintain, and develop this equipment is not common in most labs. There is therefore a significant gap between the availability of these powerful tools and the ability of investigators to access and use them efficiently. The Cell and Tissue Imaging Core was established in response to feedback from DRC center members to leverage the significant institutional investment in the newly created Washington University Center for Cellular Imaging (WUCCI) by providing an integrated approach to investigate the structure and dynamic behavior of diabetes-related cells and tissues. The overall objective of the Core is to provide access to and technical support in using advanced cellular microscopy to accelerate the pace, expand the scope, and improve the efficiency of diabetes research at Washington University. Core services meet the unique requirements of numerous investigators over a wide range of basic and translational research and attract new investigators into diabetes research. Importantly, users benefit from the in-depth diabetes and technical expertise of the Core directors and technical staff in time spent in consultation for experimental design and interpretation of data. During the past funding cycle, the Core served 67 DRC investigator research laboratories (including 14 P&F recipients), who expensed $788,342 in core recharges, representing approximately 18% of all WUCCI activity. Eighteen DRC member laboratories received micro-grant funding through the DRC Imaging Core Scholarship program. Ten of these labs were directed by junior faculty members at the rank of Assistant Professor. Thirty-five publications used and cited core services. Given the broad use of the Core over the past funding cycle, and the establishment of new imaging technologies (e.g. cryo-EM and lattice lightsheet), sample preparation services (e.g. tissue clearing), and analysis approaches (e.g. AI-based immunohistochemistry and immunofluorescent image analysis), it is anticipated that requests for Cell and Tissue Imaging Core services will continue to grow. The substantial demand by DRC members for advanced microscopic imaging and image analysis is strong evidence that the Cell and Tissue Imaging Core provides services that leverage generous institutional support and unique expertise to facilitate important and innovative diabetes research.

项目摘要：细胞和组织成像核心先进的细胞显微镜是生物研究的有力工具，在研究糖尿病的发病机制和最终治疗。成像技术已经发展过去十年中，分辨率、灵敏度和速度的迅速提高，创造了新的在活细胞中实时研究多个数量级过程的机会动物。然而，日益复杂的设备成本高昂，而且需要专业知识有效地使用、维护和开发该设备在大多数实验室中并不常见。因此有一个这些强大工具的可用性与调查人员访问和使用的能力之间存在巨大差距他们有效地。细胞和组织成像核心是根据 DRC 中心的反馈而建立的成员利用对新创建的华盛顿大学中心的重大机构投资通过提供一种综合方法来研究细胞成像（WUCCI）的结构和动态糖尿病相关细胞和组织的行为。核心的总体目标是提供对和技术支持，加快使用先进细胞显微镜的步伐，扩大范围，提高华盛顿大学糖尿病研究的效率。核心服务满足独特众多研究人员对广泛的基础研究和转化研究的需求，并吸引新的糖尿病研究的研究者。重要的是，用户受益于深入的糖尿病和技术核心董事和技术人员在实验设计和咨询方面花费的时间的专业知识数据解释。在过去的资助周期中，核心服务于 67 名 DRC 研究者的研究实验室（包括 14 个 P&F 接受者），其核心充值费用为 788,342 美元，相当于约占所有 WUCCI 活动的 18%。十八个刚果民主共和国成员实验室获得小额赠款资助通过 DRC 成像核心奖学金计划。其中十个实验室由初级教师指导助理教授级别的成员。 35 种出版物使用并引用了核心服务。鉴于在过去的融资周期中核心的广泛使用，以及新成像技术的建立（例如冷冻电镜和点阵光片）、样品制备服务（例如组织透明化）和分析方法（例如基于人工智能的免疫组织化学和免疫荧光图像分析），预计要求细胞和组织成像核心服务将继续增长。刚果民主共和国成员的大量需求先进的显微成像和图像分析有力地证明了细胞和组织成像核心提供的服务利用慷慨的机构支持和独特的专业知识来促进重要和创新的糖尿病研究。