Cell and Tissue Imaging Core

细胞和组织成像核心

• 批准号: 10197894
• 负责人: James Alexander Fitzpatrick
• 金额: $ 15.32万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-24 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10197894
• 关键词: 3-Dimensional; Algorithmic Software; Animals; Basic Science; Behavior; Biological Assay; Books; Cells; Computer software; Consultations; Cryo-electron tomography; Cryoelectron Microscopy; Custom; Data; Data Analyses; Electrons; Elements; Ensure; Equipment; Experimental Designs; Face; Fees; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Generations; Goals; Human Resources; Image; Image Analysis; Imaging technology; Investments; Ions; Liver Fibrosis; Location; Logistics; Measurement; Methodology; Microscope; Microscopic; Microscopy; Microtomy; Mission; Modality; Morphology; Negative Staining; Online Systems; Optics; Organism; Pathogenesis; Pathology; Play; Preparation; Process; Proteins; Reagent; Research; Research Personnel; Resolution; Resources; Roentgen Rays; Role; Sampling; Scanning; Scanning Electron Microscopy; Services; Slide; Speed; Structure; Techniques; Technology; Time; Tissue imaging; Tissues; Training; Translational Research; Universities; Visualization; Washington; X ray microscopy; base; biological research; cellular imaging; data resource; data sharing; design; experimental study; human disease; imaging modality; instrument; instrumentation; live cell microscopy; medical schools; microscopic imaging; novel therapeutics; particle; programs; ratiometric; research study; tissue culture; tool; translational study; transmission process; two photon microscopy; two-dimensional; two-photon

PROJECT SUMMARY: Cell and Tissue Imaging Core A Advanced cellular microscopy is a powerful tool for biological research and has an important role to play in the study of the pathogenesis and eventual treatment of human diseases. Imaging technology has evolved rapidly over the last decade leading to improvements in resolution, sensitivity and speed which have created fundamentally new opportunities for studying processes across many orders of magnitude and in real-time in living cells and organisms. The Cell and Tissue Imaging Core A is comprised of a shared cutting-edge multi-scale microscopy facility that is essential to the research goals of the 3 projects in this P01 renewal application entitled “New Therapies for liver fibrosis and hyperproliferation in alpha1-AT deficiency (ATD)”. The facility is housed within the Washington University Center for Cellular Imaging (WUCCI) (http://wucci.wustl.edu), to leverage recent significant institutional investments at Washington University School of Medicine as well as to provide an integrated approach to investigating the structure and dynamic behavior of ATD specific cells and tissues. All projects within this P01 renewal plan to make heavy use of the diverse array of cellular microscopy approaches available in the WUCCI. Methodologies available to project PIs include optical microscopy modalities such as fluorescence microscopy, confocal and two- photon microscopy, live-cell microscopy (both confocal and Total Internal Reflection - TIRF), super- resolution microscopy (both PALM / STORM and SIM) and slide-scanning microscopy (both bright-field and fluorescence). Additional advanced fluorescence modalities such as FRET, FRAP, spectral and ratiometric imaging are also available. A complete array of ultrastructural approaches is also available to project investigators. These comprise both transmission and scanning electron microscopy (TEM and SEM), negative staining, traditional thin section EM, immuno-EM, serial block face Focused Ion Beam-SEM (FIB- SEM), X-ray microscopy, single particle cryo-EM and cryo-Electron Tomography (ET). Furthermore, the facility provides access to a wide range of image analysis and quantitation software as well as technical assistance in their appropriate use from facility staff. Since the facility, the equipment and the staff expertise reside in one contiguous location, seamless integration between the imaging modalities can be realized. The facility is accessible ID card 24/7 by trained users who can also book instrument time or submit sample- processing requests anytime through our on-line web-based e-management solution, Agilent CrossLab (formerly iLab solutions).

项目摘要：细胞和组织成像核心 A 先进的细胞显微镜是生物研究的强大工具，在 人类疾病的发病机制和最终治疗的研究已经发展。 过去十年中，分辨率、灵敏度和速度迅速提高， 为研究跨多个数量级和领域的过程创造了根本性的新机会 细胞和组织成像核心 A 由一个共享的单元组成。 尖端的多尺度显微镜设施对于本研究中 3 个项目的研究目标至关重要 P01 续展申请，标题为“α1-AT 肝纤维化和过度增殖的新疗法” 该设施位于华盛顿大学细胞成像中心。 (WUCCI) (http://wucci.wustl.edu)，利用华盛顿最近的重大机构投资 大学医学院以及提供综合方法来研究结构和 ATD 特定细胞和组织的动态行为此 P01 更新计划中的所有项目都变得繁重。 使用 WUCCI 方法中提供的各种细胞显微镜方法。 投影 PI 包括光学显微镜模式，例如荧光显微镜、共焦显微镜和双 光子显微镜、活细胞显微镜（共焦和全内反射 - TIRF）、超 分辨率显微镜（PALM / STORM 和 SIM）和幻灯片扫描显微镜（明场和 其他先进的荧光模式，例如 FRET、FRAP、光谱和比率测量 还可以使用一系列完整的超微结构方法进行投影。 研究人员包括透射电子显微镜和扫描电子显微镜（TEM 和 SEM）， 负染色、传统薄切片 EM、免疫 EM、连续块面聚焦离子束 SEM (FIB- SEM）、X 射线显微镜、单颗粒冷冻电镜和冷冻电子断层扫描 (ET)。 该设施提供各种图像分析和定量软件以及技术 设施工作人员协助适当使用设施、设备和工作人员的专业知识。 驻留在一个连续位置，可以实现成像模式之间的无缝集成。 经过培训的用户可以通过 ID 卡 24/7 随时使用该设施，他们还可以预订仪器时间或提交样品 - 通过我们基于网络的在线电子管理解决方案 Agilent CrossLab 随时处理请求 （以前称为 iLab 解决方案）。