BASAL GANGLIA--CHEMICAL AND FUNCTIONAL CIRCUITRY

基底神经节——化学和功能电路

• 批准号: 2264530
• 负责人: Suzanne N Haber
• 金额: $ 22.79万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1997-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2264530
• 关键词: Macaca; basal ganglia; brain mapping; central neural pathway /tract; enkephalins; immunocytochemistry; in situ hybridization; intercellular connection; neural information processing; neuroanatomy; neurochemistry; neuronal transport; neurophysiology; neurotransmitter transport; substantia nigra

Patients with diseases of the basal ganglia have a number of debilitating disorders that involve a combination of motoric, cognitive, and emotional symptoms. The basal ganglia are a set of structures known for their interconnectivity, and loops systems. Based on the anatomy and physiology of the motor loops, it has been suggested that the basal ganglia and frontal cortex are organized in parallel, functionally segregated circuits. Each circuit involves a specific, functionally distinct region of the frontal cortex and projects to anatocally distinct sectors of the striatum, the pallidum, the substantia nigra, and the thalamus. Our anatomical work has suggested ways in which the ventral (or limbic-related parts of the basal ganglia) may interface with the motor circuits to produce the constellation of symptoms seen in basal ganglia diseases. The main objective of this proposal is to test this idea and to further define the role of the ventral basal ganglia in modulating general basal ganglia output by the anatomical pathways involved. In particular, our results show that: the dorsal striatum may be modulated by inputs from the ventral striatum and ventral pallidum. In addition, unlike rats, the main efferent projection of the ventral pallidum, outside of basal ganglia structures is not to the thalamus but to the lateral habenular nucleus. The source of these fibers is controversial but is likely to be derived from the ventral striatum. The connections of this nucleus are therefore important to know in order to understand how information flows through the ventral basal ganglia. Finally, enkephalin immunoreactivity is dense in the substantia nigra, pars reticulata. The source of these fibers is controversial but is likely to be derived from the ventral striatum. We will use intracellular injections and circuit tracing techniques to identify pathways, and immunohistochemistry and in situ hybridization to colocalize relevant transmitters in particular pathways. Tracers will be injected to study: the extent to which the ventral pallidum and ventral striatum anatomically modulates the dorsal striatum; the connections of the lateral habenular nucleus; and whether there is an enkephalinergic projection from the ventral striatum to the substantia nigra.

基底神经节疾病的患者有许多令人沮丧的 涉及摩托车，认知和情感的疾病 症状。基底神经节是一组以其闻名的结构 互连和循环系统。 基于解剖学和生理 在电动机环中，有人建议基底神经节和 额叶皮层在功能上隔离的电路并行组织。 每个电路涉及一个特定的功能不同区域 额叶皮层并投射到纹状体的解剖区域， Pallidum，底底尼格拉和丘脑。我们的解剖学工作 已经提出了腹侧（或与边界相关的部分的方式） 基底神经节）可以与电机电路接口以产生 基底神经节疾病中看到的症状星座。主 该建议的目的是测试这个想法，并进一步定义 腹底神经节在调节一般基底神经节中的作用 涉及的解剖途径输出。特别是，我们的结果表明 那就是：背纹状体可以通过腹侧的输入来调节 纹状体和腹侧颗粒。此外，与大鼠不同 基底神经节结构以外的腹侧颗粒的投影是 不是丘脑，而是到侧向核核。 的来源 这些纤维引起了争议，但很可能是从腹侧派生的 纹状体。因此，该核的联系很重要。 为了了解信息如何流过腹侧的基础 神经节。 最后，脑脑免疫反应性在本质中致密 Nigra，pars reticulata。这些纤维的来源是有争议的，但 可能源自腹侧纹状体。 我们将使用细胞内注射和电路跟踪技术 识别途径，免疫组织化学以及原位杂交 在特定途径中将相关发射器进行共定位。 示踪剂会 注入研究：腹侧颗粒和腹侧的程度 纹状体在解剖学上调节背纹状体；连接 侧界核；以及是否有脑磷脂能 从腹侧纹状体到黑质的投射。