Cellular Imaging Core

细胞成像核心

基本信息

批准号：
10019351
负责人：
James Alexander Fitzpatrick
金额：
$ 22.14万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10019351
关键词：
3-Dimensional 4D Imaging Animal Model Animals Area Basic Science Biological Assay Biological Models Cell physiology Cells Characteristics Consultations Consumption Contrast Media Custom Data Analyses Data Set Development Disease Educational workshop Electron Microscopy Electrons Ensure Equipment Experimental Designs Feedback Funding Generations Goals Human Resources Image Image Analysis Imaging Techniques Imaging technology Immunofluorescence Microscopy Individual Informatics Investments Ions Joints Knowledge Laboratories Laboratory Research Light Logistics Methodology Microscope Microscopic Microscopy Morphology Optics Organ Pathogenesis Play Preparation Process Research Research Personnel Resolution Resources Rheumatism Roentgen Rays Role Sampling Services Specialist Specimen Speed Structure System Technical Expertise Techniques Technology Time Tissue imaging Tissues Training Translating Translational Research Universities Visualization Washington Work X ray microscopy base biological research cell behavior cellular imaging cost data acquisition imaging modality imaging study improved innovation instrumentation light microscopy member microscopic imaging molecular imaging novel operation programs quantitative imaging research study response single molecule soft tissue tool translational study two-dimensional two-photon

项目摘要

PROJECT SUMMARY: Cellular Imaging Core Advanced cellular microscopy is a powerful tool for biological research and has an important role to play in the study of disease pathogenesis, which may translate eventually to novel treatment approaches for rheumatic diseases. Imaging technology has evolved rapidly over the last decade leading to improvements in resolution, sensitivity and speed, which have created fundamentally new opportunities for studying processes across many orders of magnitude and in real-time in living cells and animals. At the same time, the costs of increasingly sophisticated equipment are substantial and the expertise to efficiently use, maintain, and develop this equipment is not common in most labs. There is, therefore, a significant gap between the availability of these powerful tools and the ability of individual investigators to access and use them efficiently. The Cellular Imaging Core was created in response to feedback from Rheumatic Diseases Research Resource-based Center members to leverage the significant institutional investment in the newly created Washington University Center for Cellular Imaging (WUCCI) and provide an integrated approach to investigate the structure and dynamic behavior of cells and tissues rheumatic disease-related model systems. The overall objective of the Core is to provide access to and technical support in using advanced cellular microscopy tools to accelerate the pace, expand the scope, and improve efficiency of rheumatic diseases research. The Core services meet the unique requirements of numerous investigators over a wide range of basic and translational research, attracting new investigators into rheumatic diseases research areas. Importantly the users benefit from the in-depth technical expertise of the Core Director and technical staff in the time spent in consultation for experimental design and interpretation of data. During the last fiscal year (July 1 2016 – June 30 2017) of WUCCI operations, the Core served 184 research laboratories, 11 of these were RDRRC members whose usage represented ~10% of the overall core consumption. It is anticipated that requests for Cellular Imaging Core services will continue to grow given the overwhelming need for advanced microscopic imaging and image analysis approaches in the study of rheumatic diseases. The Cellular Imaging Core will provide essential services that will enable and accelerate the research of RDRRC investigators throughout the next funding cycle.

项目摘要：细胞成像核心先进的细胞显微镜是生物研究的强大工具，在对疾病发病机制的研究，最终可能转化为新的治疗方法风湿性疾病的成像技术在过去十年中迅速发展，带来了进步。分辨率、灵敏度和速度，这为研究创造了全新的机会同时，在活细胞和动物中进行多个数量级的实时过程。日益复杂的设备的成本是巨大的，而有效利用的专业知识，维护和开发这种设备在大多数实验室中并不常见，因此存在很大的差距。这些强大工具的可用性与个别调查人员访问和使用的能力之间的关系细胞成像核心是根据风湿性疾病的反馈而创建的。研究资源中心成员利用重大机构投资在新领域创建了华盛顿大学细胞成像中心 (WUCCI)，并提供了一种综合方法研究细胞和组织风湿性疾病相关模型的结构和动态行为核心的总体目标是提供使用先进系统的访问和技术支持。细胞显微镜工具加快风湿病诊断步伐、扩大范围、提高效率疾病研究的核心服务满足了众多研究人员的独特需求。一系列基础和转化研究，吸引新的研究者进入风湿病研究重要的是，用户可以从核心总监和技术人员的深入技术专业知识中受益。工作人员在最后期间花在咨询实验设计和数据解释上的时间。 WUCCI 运营的财政年度（2016 年 7 月 1 日至 2017 年 6 月 30 日），核心服务于 184 个研究实验室，其中 11 个是 RDRRC 成员，其使用量约占总体核心消耗的 10%。鉴于压倒性的压力，预计对蜂窝成像核心服务的需求将继续增长风湿性疾病研究需要先进的显微成像和图像分析方法。细胞成像核心将提供必要的服务，以促进和加速细胞成像的研究 RDRRC 调查员将参与下一个资助周期。