Claude D. Pepper Older Americans Independence Center

克劳德·D·佩珀美国老年人独立中心

• 批准号: 7814804
• 负责人: Marco Pahor
• 金额: $ 21.97万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7814804
• 关键词: Address; Aerobic; Agriculture; American; Arts; Behavioral; Bioinformatics; Biological; Biological Markers; Biometry; Budgets; Cessation of life; Clinical Research; Communities; Complement; Complex; County; Data; Economics; Elderly; Electron Transport; Environment; Exons; Failure; Fatigue; Florida; Food; Genetic; Genomics; Health; Homeostasis; Impairment; In Situ; Individual; Institutes; Iron; Laser Scanning Microscopy; Lead; Magnetic Resonance Imaging; Medical; Metabolism; Mitochondria; Modification; Monitor; Muscle; Muscle function; Occupations; Older Population; Oxygen; Oxygen Consumption; Phenotype; Physical Function; Physiological; Pilot Projects; Prevention; Process; Production; Psychosocial Assessment and Care; Psychosocial Factor; Quality of life; Rehabilitation therapy; Reporting; Research; Research Infrastructure; Research Personnel; Resolution; Respiration; Risk; Science; Services; Skeletal Muscle; Symptoms; Unemployment; Universities; age related; base; consumption measures; data management; depression; disability; economic impact; exhaustion; functional disability; gel electrophoresis; genome wide association study; improved; in vivo; mitochondrial dysfunction; new technology; novel strategies; parent grant; psychologic; psychosocial; public health relevance; residential care facility; respiratory; sarcopenia

DESCRIPTION (provided by applicant): This revision expands the focus of the OAIC to "mitochondrial function and fatigue". Fatigue is a major complaint among older adults and is strongly associated with loss of independence and impairments regardless of underlying medical conditions. Multiple biological and psychosocial factors may contribute to or influence fatigue. In particular, skeletal muscle mitochondrial dysfunction can lead to reduced energy production, which could result in fatigue. The new focus on mitochondrial function and fatigue will complement and enhance the sarcopenia theme of the parent grant. To address this new focus we propose the revision of three OAIC cores with the addition of new assessments and a new pilot study. The Clinical Research Core will include oxygen consumption measures to assess whole body energy efficiency and utilization (V02peak), in vivo MRI assessments of mitochondrial function, activity monitoring, and comprehensive health and psychosocial assessments of fatigue to characterize the phenotype of fatigue. The Genomics, Metabolism and Biomarkers Core will provide the following novel technologies: (a) intravital multiphoton excitation laser-scanning microscopy to obtain assessments of mitochondrial function, and iron (Fe) accumulation and localization, (b) high-resolution respirometry to assess mitochondrial respiration, and electron transport chain and OXPHOS capacity in situ, (c) Blue native gel electrophoresis (BN PAGE) to determine content and enzymatic activity of the individual mitochondrial respiratory complexes, and (d) array-based genomic analysis to identify genotypic and phenotypic differences correlated with muscle mitochondrial function and iron (Fe) homeostasis, utilizing genome-wide association studies and exon expression analysis. The Biostatistics and Data Management Core will implement bioinformatics and analyses of genomic data. Economic impact The University of Florida (UF) contributes substantially to the local and regional economy. In 2008, UF created 2,525 jobs and recent studies have shown that UF contributes nearly $6 billion annually to Florida's economy. The university employs about 34,000 people directly on its main campus and via UF organizations, such as the Institute of Food and Agricultural Sciences, is responsible for the creation of 74,894 jobs statewide. Unfortunately, because of the current economic environment, as of April 16*, 2009 UF is required to cut $120 million from its budget. This cut will raise local unemployment rates and will vibrate across many surrounding counties. The current proposal will create or retain 8 jobs. PUBLIC HEALTH RELEVANCE: Fatigue is a common symptom in older populations and is often associated with functional limitations, impairments and disability. Understanding the biological mechanisms underlying fatigue may help to devise novel approaches to avert fatigue and improve quality of life and independence of older adults.

描述（由申请人提供）：此修订将OAIC的重点扩展到“线粒体功能和疲劳”。疲劳是老年人的主要抱怨，与失去独立性和障碍无关，无论其潜在的医疗状况如何。多种生物学和社会心理因素可能导致或影响疲劳。特别是，骨骼肌线粒体功能障碍会导致能量产生降低，这可能导致疲劳。对线粒体功能和疲劳的新关注将补充和增强父母赠款的肌肉减少主题。为了解决这个新的重点，我们建议对三个OAIC核心进行修订，并增加新的评估和一项新的试点研究。临床研究核心将包括评估全身能源效率和利用率的氧气消耗措施（V02PEAK），对线粒体功能的体内MRI评估，活动监测以及疲劳的全面健康和心理心理评估，以表征疲劳表型的表征。基因组学，代谢和生物标记物核心将提供以下新技术：（a）内内室内多光激发激光扫描显微镜以获得线粒体功能的评估，以及铁（FE）积累和定位，（B）高分辨率呼​​吸量，以评估线粒体呼吸器和电式蓝色的蓝色电位蓝色，并在旋转的旋转能力（b）。 （BN页面）确定单个线粒体呼吸系统复合物的含量和酶活性，以及​​（d）基于阵列的基因组分析，以识别与肌肉线粒体功能和铁（FE）稳态相关的基因型和表型差异，利用了全基因组范围的关联研究和外生的表达分析。生物统计学和数据管理核心将实施基因组数据的生物信息学和分析。经济影响佛罗里达大学（UF）对地方和地区经济产生了重大贡献。 2008年，UF创造了2,525个就业机会，最近的研究表明，UF每年为佛罗里达州的经济贡献近60亿美元。该大学直接在其主要校园内和通过UF组织（例如食品和农业科学研究所）雇用约34,000名员工，负责全州创建74,894个职位。不幸的是，由于当前的经济环境，截至2009年4月16日*，UF必须从预算中削减1.2亿美元。这种削减将提高当地的失业率，并在许多周围县的振动中振动。当前的建议将创造或保留8个工作。 公共卫生相关性：疲劳是老年人群中的常见症状，通常与功能限制，障碍和残疾有关。了解疲劳的生物学机制可能有助于设计出避免疲劳并改善老年人生活质量和独立性的新方法。