CORE--TRANSGENIC MOUSE

核心——转基因小鼠

• 批准号: 7651455
• 负责人: Robert C Young
• 金额: $ 15.31万
• 依托单位: FOX CHASE CANCER CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7651455
• 关键词: Accidents; Address; Animal Model; Animals; Biological Models; Biology; Calendar; Cryopreservation; DNA; Development; Developmental Biology; Developmental Therapeutics Program; Differentiation and Growth; Disease; ES Cell Line; Event; Fox Chase Cancer Center; Funding; Future; Gene Expression Regulation; Gene Targeting; Genes; Health Services Accessibility; Human; Human Genetics; Immune system; Immunobiology; Insurance; Knockout Mice; Malignant Neoplasms; Malignant neoplasm of ovary; Mouse Strains; Mus; Numbers; Oncogenes; Peer Review; Play; Productivity; Rate; Recruitment Activity; Research; Research Personnel; Resources; Role; Services; Study models; Transgenic Mice; Transgenic Mouse Facility; Transgenic Organisms; Tumor Cell Biology; cancer therapy; gene function; insight; knockout animal; knockout gene; pressure; programs; success; tumorigenesis

The ability to introduce manipulated genes and delete or modify endogenous genes in the germline of animals is a major technological advance in biology, enabling investigators to answer questions about gene function which must be analyzed in a whole animal model system. Transgenic and knockout animals have been instrumental in providing insights into mechanisms of developmental gene regulation, cellular interactions within the immune system, and the effect of oncogenes on growth and differentiation. Demand for these services has grown significantly since the last review, approximately doubling every two years. A new Facility Manager was recruited in early 2000 with a specific expertise in generating knockout mice. In calendar year 1999, there were 21 requests for services. In 2003, 84 requests were completed (76 DNA Constructs Injected, 8 ES Cell Lines Injected). The number of peer-reviewed funded users has increased from nine at the last review to 15 in calendar year 2003. The use from these 15 peer-reviewed funded investigators represents six different Research Programs and all three Divisions of the Fox Chase Cancer Center (FCCC). Ninety-nine percent (99%) of Facility usage is by peer-reviewed funded investigators. As described in the last application, we planned to add needed cryopreservation services. Cryopreservation has been established and has become a routine service. It is currently used by 11 investigators. A total of 88 lines have been preserved to date. The availability of this service has resulted in several clear benefits, including the ability to bank mouse lines for future study, to relieve pressure on existing mouse colony space, to provide a means for propagating reproductively incompetent mouse lines, and to provide insurance against the loss of irreplaceable mouse lines should a catastrophic event occur in the mouse colony. The previous review noted a need to provide cryopreservation and gene knockout services as well as to increase Facility usage. All three points have been fully addressed. Both cryopreservation and gene targeting are now accomplished routinely, with a 100% success rate. Productivity and usage have increased by 200% and 100%, respectively, since 1998.

引入操纵基因并删除或修改内源基因的能力 动物种系是生物学的一项重大技术进步，使研究人员能够回答 有关基因功能的问题必须在整个动物模型系统中进行分析。 转基因和基因敲除动物有助于深入了解发育基因调控机制、免疫系统内的细胞相互作用以及癌基因对生长和分化的影响。自上次审查以来，对这些服务的需求显着增长，大约每两年翻一番。 2000 年初，新聘用了一位在基因敲除小鼠生成方面具有特殊专业知识的设施经理。 1999 历年，有 21 项服务请求。 2003 年，完成了 84 个请求（注入了 76 个 DNA 构建体，注入了 8 个 ES 细胞系）。同行评审资助的用户数量已从上次评审时的 9 名增加到 2003 年的 15 名。这 15 名同行评审资助的研究人员的使用代表了福克斯蔡斯癌症中心 (FCCC) 的 6 个不同的研究项目和所有三个部门。 ）。百分之九十九 (99%) 的设施使用是由同行评审资助的研究人员进行的。正如上一个申请中所述，我们计划添加所需的冷冻保存服务。冷冻保存已经建立并成为一项常规服务。目前有 11 名研究人员使用它。至今共保存88行。这项服务的可用性带来了几个明显的好处，包括能够储存小鼠品系以供将来研究、减轻现有小鼠群体空间的压力、提供繁殖无能力小鼠品系的方法以及提供针对损失的保险。如果小鼠群体中发生灾难性事件，则需要使用不可替代的小鼠品系。之前的审查指出需要提供低温保存和基因敲除服务以及增加设施的使用。这三点都已得到充分解决。冷冻保存和基因打靶现在都已常规完成，成功率达 100%。自 1998 年以来，生产率和使用率分别提高了 200% 和 100%。