Core 1: Tissue Pathology and Cellular Analysis

核心 1：组织病理学和细胞分析

• 批准号: 10443608
• 负责人: MARY Kay WASHINGTON
• 金额: $ 31.13万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-09 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10443608
• 关键词: Academic Medical Centers; Animal Cancer Model; Antibodies; Basic Science; Behavior; Bioinformatics; Biological Assay; Biological Process; Biometry; Cancer Center; Cancer Science; Cells; Collaborations; Collection; Colorectal Cancer; Colorectal Neoplasms; Complement; Computer Analysis; Confidentiality of Patient Information; Consult; Consultations; Cooperative Human Tissue Network; Custom; Cytometry; Data; Data Analyses; Databases; Diagnosis; Digestive System Disorders; Disease; Early Diagnosis; Ensure; Epithelial; Epithelial Cells; Evaluation; Formalin; Freezing; Gastrointestinal Neoplasms; Gene Expression; Generations; Histologic; Histology; Histopathology; Human; Image; Immunofluorescence Immunologic; Immunohistochemistry; Informatics; Informed Consent; Infrastructure; Malignant neoplasm of gastrointestinal tract; Mission; Molecular; Morphology; Mus; Normal tissue morphology; Paraffin Embedding; Pathology; Patient Care; Patients; Play; Pre-Clinical Model; Preparation; Prevention; Process; Protein Analysis; Publishing; Quality Control; Reagent; Research; Research Activity; Research Personnel; Resource Sharing; Role; Running; Sampling; Services; Signal Transduction; Specimen; Technology; The Vanderbilt-Ingram Cancer Center at the Vanderbilt University; Time; Tissue Banks; Tissue Microarray; Tissue Sample; Tissues; Translational Research; Validation; Washington; Work; biobank; cancer biomarkers; cellular imaging; colorectal cancer treatment; cost effective; data de-identification; data quality; digital; flexibility; human disease; image processing; instrument; laser capture microdissection; molecular imaging; mouse model; next generation; novel; operation; protein expression; repository; screening; single cell analysis; single-cell RNA sequencing; synergism; tool; translational goal

Project Summary/Abstract: Tissue Pathology and Cellular Analysis Core The Translational Pathology and Cellular Analysis (TPCA) Core, will play a central role in the translational research mission of this SPORE, providing essential support for all projects by providing annotated human biospecimens and research histology services. It includes with a single-cell analysis subcore under the direction of Dr. Ken Lau. This subcore will enable technologies for quantifying and analyzing protein expression at a multiplex, single-cell level, greatly augmenting the histopathology capabilities of the TPCA on both FFPE, fresh, and frozen tissue samples. While the primary mission of the TPCA is state-of-the-art immunohistochemistry, research pathology and biorepository services, the single-cell analysis subcore will offer three cutting-edge technologies: inDrop single-cell RNA-Seq, MxIF, and DISSECT-CyTOF, and their associated computational analyses. The single-cell analysis subcore will offer services, from the preparation and processing of samples, the generation and verification of epithelial-specific reagents, screening and validation of targets, and finally, computational data analysis. Single-cell data will be synthesized and presented to investigators in adaptable formats for presentation and/or downstream analysis. A specific strength of this subcore in relation to the GI SPORE is that Dr. Ken Lau's research focus is on GI disorders and his expertise will greatly synergize with the research of GI neoplasms. We can offer highly specific molecular assays that can flexibly query a wide range of biological processes including the evaluation of signaling, cell identity, function, and behavior, all within a single cell. The TPCA will partner with other established tissue collection and immunohistochemistry shared resources, such as the Vanderbilt University Medical Center Translational Pathology Shared Resource and the Digital Histology Shared Resource, to provide exceptional services to the GI SPORE in a highly cost-effective manner. Close collaboration with the Biostatistics and Bioinformatics Core will provide the TPCA and GI SPORE with robust informatics and database tools for collection, distribution, and annotation of biospecimens in the GI SPORE repository. Dr. Washington and Dr. Lau have developed synergistic working relationships with imaging services under the direction of Dr. Charles Manning and these will be maintained between the TPCA and the Mouse and Human Molecular Imaging Core.

项目摘要/摘要：组织病理学和细胞分析核心 翻译病理学和细胞分析（TPCA）核心将在翻译中发挥核心作用 该孢子的研究任务，通过提供注释的人来为所有项目提供基本的支持 生物测量和研究组织学服务。它在指导下包含单细胞分析子核 Ken Lau博士。该子核心将启用技术来量化和分析A的蛋白质表达 多路复用，单细胞水平，极大地增强了TPCA在FFPE上的组织病理学能力， 和冷冻的组织样品。尽管TPCA的主要任务是最先进的免疫组织化学，但 研究病理学和生物验证服务，单细胞分析子核心将提供三个尖端 技术：Indrop单细胞RNA-SEQ，MXIF和DISSECT-CYTOF及其相关的计算 分析。单细胞分析子核心将提供服务，从样本的准备和处理， 对靶标的上皮特异性试剂的产生和验证，最后，筛选和验证 计算数据分析。单细胞数据将被合成并提交给研究人员的适应性 用于演示和/或下游分析的格式。与GI相关的该子核心的特定强度 Spore是Ken Lau博士的研究重点是胃肠道疾病，他的专业知识将与 胃肠道肿瘤的研究。我们可以提供高度特定的分子测定，可以灵活地查询各种各样的 生物学过程，包括评估信号，细胞身份，功能和行为，全部在单个内部 细胞。 TPCA将与其他已建立的组织收集和免疫组织化学合作，共享资源， 例如范德比尔特大学医学中心转化病理学共享资源和数字资源 组织学共享资源，以高成本有效的方式为GI孢子提供卓越的服务。 与生物统计学和生物信息学核心的密切合作将为TPCA和GI孢子提供 GI中生物测量的收集，分发和注释的强大信息学和数据库工具 孢子存储库。华盛顿博士和劳博士与成像建立了协同的工作关系 在查尔斯·曼宁（Charles Manning）博士的指导下的服务，这些服务将在TPCA和 小鼠和人类分子成像核心。