Vitamin D and Natural History of Human Papillomavirus (HPV) Infections in Women

维生素 D 与女性人乳头瘤病毒 (HPV) 感染的自然史

基本信息

批准号：
9280589
负责人：
RACHEL L. WINER
金额：
$ 23.25万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-07-14 至 2019-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9280589
关键词：
25-hydroxyvitamin D Achievement Adult Affect Age-Years Anogenital cancer Bioavailable Biological Markers Cervical Chronic Disease Communicable Diseases DNA DNA Damage Data Development Diet Dihydroxycholecalciferols Future Genotype Glean HPV-High Risk Health behavior Hormones Human papilloma virus infection Immune Infection Laboratories Link Longitudinal Studies Maintenance Malignant neoplasm of cervix uteri Measurement Measures Methods Minerals Mutation National Health and Nutrition Examination Survey Natural History Nutrient Nutritional status Play Questionnaires Recruitment Activity Reporting Research Research Design Risk Role Sampling Serologic tests Serological Serum Severities Sex Behavior Testing Therapeutic Intervention Time Vagina Vitamin D Vitamin D Deficiency Vitamins Woman adaptive immune response base carcinogenesis clinical practice cohort prevent sexually active

项目摘要

PROJECT DESCRIPTION Co-factors linked to high-risk (hr) HPV persistence versus transience are not well-defined. Deficiencies in nutrients such as vitamin D can increase the risk of carcinogenesis via DNA damage, immune incompetence, and genetic alterations. While a recent study reported that vitamin D deficiency was associated with an increased likelihood of prevalent hrHPV infection, to our knowledge, no study has explored associations between vitamin D levels and hrHPV persistence or reactivation. From 2011-2012, we recruited 409 healthy mid-adult women to participate in a 6-month longitudinal study of hrHPV infections. Each month, women self- collected vaginal samples for PCR-based genotype-specific hrHPV DNA testing and completed health and sexual behavior questionnaires. In addition, sera were collected for hrHPV serology testing and future biomarker testing. We propose to make efficient use of stored sera, monthly hrHPV genotyping results, and hrHPV serology results to evaluate vitamin D levels in relation to presence, persistence, and reactivation of hrHPV infection. To explore different potential mechanisms of vitamin D involvement, sera will be tested for several different biomarkers: 25-hydroxyvitamin D (25(OH)D) and several alternative measures that may better characterize vitamin D sufficiency, including 1,25-dihydroxyvitamin D (1,25(OH)2D), free vitamin D, and bioavailable vitamin D. Our aims are to: 1) Evaluate cross-sectional associations between serum vitamin D levels and prevalent hrHPV infection; 2) Evaluate associations between serum vitamin D levels and persistent vs. transient type-specific hrHPV infection; and 3) In women with evidence of prior hrHPV infection at baseline (positive serology / negative DNA), evaluate the association between serum vitamin D levels and subsequent reactivation of the same hrHPV type. We hypothesize that higher serum vitamin D levels will be inversely associated with prevalent, persistent, and reactivated hrHPV infection. Information gleaned will help us to understand whether serum vitamin D levels impact the hrHPV natural history in mid-adult women. Ultimately, these pilot data will be crucial in developing future longitudinal studies designed to evaluate whether achievement or maintenance of sufficient serum vitamin D levels can positively alter the natural history of hrHPV infections.

项目描述与高危 (hr) HPV 持续性和短暂性相关的辅助因素尚未明确定义。不足之处维生素 D 等营养物质会因 DNA 损伤、免疫功能低下、和基因改变。虽然最近的一项研究报告称，维生素 D 缺乏与据我们所知，流行 hrHPV 感染的可能性增加，尚无研究探讨关联性维生素 D 水平与 hrHPV 持续存在或再激活之间的关系。 2011-2012年，我们招募了409名健康中成年女性参加一项为期 6 个月的 hrHPV 感染纵向研究。每个月，女性都会自我收集阴道样本进行基于 PCR 的基因型特异性 hrHPV DNA 检测，并完成健康和性行为问卷。此外，收集血清用于 hrHPV 血清学检测和未来生物标志物测试。我们建议有效利用储存的血清、每月 hrHPV 基因分型结果，以及 hrHPV 血清学结果可评估维生素 D 水平与病毒存在、持续性和重新激活的关系 hrHPV 感染。为了探索维生素 D 参与的不同潜在机制，将对血清进行测试几种不同的生物标志物：25-羟基维生素 D (25(OH)D) 和几种可能更好的替代措施表征维生素 D 充足性，包括 1,25-二羟基维生素 D (1,25(OH)2D)、游离维生素 D 和生物可利用的维生素 D。我们的目标是：1) 评估血清维生素 D 之间的横截面关联水平和流行的 hrHPV 感染； 2) 评估血清维生素 D 水平与持久性之间的关联与暂时性类型特异性 hrHPV 感染相比； 3) 在基线时有 hrHPV 感染证据的女性（血清学阳性/DNA 阴性），评估血清维生素 D 水平与后续结果之间的关联相同 hrHPV 类型的重新激活。我们假设较高的血清维生素 D 水平将呈反比与流行、持续和重新激活的 hrHPV 感染有关。收集到的信息将帮助我们了解血清维生素 D 水平是否影响中成年女性的 hrHPV 自然史。最终，这些试点数据对于开展未来的纵向研究至关重要，这些研究旨在评估是否达到或维持足够的血清维生素 D 水平可以积极改变自然史 hrHPV 感染。