Request for transmission electron microscope for the University of Pittsburgh

匹兹堡大学申请透射电子显微镜

• 批准号: 8447178
• 负责人: SIMON C WATKINS
• 金额: $ 45.9万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447178
• 关键词: 18 year old; Area; Communities; Computers; Core Facility; Country; Electron Microscope; Electron Microscopy; Electrons; Engineering; Failure; Frustration; Funding; Housing; Image; Image Analysis; Institution; Life; Light; Mechanics; Medical; Medical Research; Medicine; Methods; Microscope; Research; Research Personnel; Research Project Grants; Schools; Services; Time; Universities; Vacuum; base; instrument; lens; operation; optical imaging; transmission process

DESCRIPTION (provided by applicant): This request is for funds to purchase a JEOL 1400 transmission electron microscope. This instrument will be housed within the Center for Biologic Imaging (CBI) at the University Of Pittsburgh School Of Medicine. The mandate of this core facility is to provide access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University Of Pittsburgh School Of Medicine. Electron microscopy is an essential service provided by the center, the current core use electron microscopes within the facility are a JEOL 1011 and 1210. The 1011 is operating at capacity. The 1210 is 18 years old and reaching the end of its useable life. In the last year we have had multiple significant failures, these related to camera problems, a variety of computer failures, general vacuum failures and other mechanical (lens) failures. In each case these failures tend toward catastrophic rather than trivial because quite simply the engineers do not know how to fix the problems and the parts needed are either very rare or cannot be found. These failures are difficult to deal with as the use of EM within the center has continued to grow such that we are no longer able to satisfy user needs without two fully functional microscopes. Therefore, the reasons for this application are to: 1: Provide critically needed instrument time; 2 Replace the 1210 instrument which is no longer serviceable as a core microscope. Since the CBI commenced operations over 20 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 250 PHS funded groups within the medical area, as well as in PHS supported projects with investigators at institutions around the country. As the PHS funded user base continues to expand, a lack of quality EM time has become a major limiting factor in the utility of the center, this leads to considerable frustration amongst users.

描述（由申请人提供）：此请求是要购买JEOL 1400传输电子显微镜的资金。该乐器将安置在匹兹堡大学医学院的生物成像中心（CBI）中。该核心设施的任务是为匹兹堡大学医学院的所有研究小组提供全方位的光和电子光学，图像分析和形​​态学方法。 电子显微镜是由中心提供的必需服务，该设施内的当前核心使用电子显微镜为JEOL 1011和1210。1011以容量运行。 1210岁的年龄是18岁，并达到了其可用寿命的终结。在过去的一年中，我们发生了多次重大故障，这些故障与摄像机问题，各种计算机故障，一般真空失败和其他机械（镜头）故障有关。在每种情况下，这些失败趋于灾难性而不是微不足道，因为工程师不知道如何解决问题，所需的零件很少或找不到。这些失败很难处理，因为中心内的使用EM的使用持续增长，因此我们不再能够在没有两个功能齐全的显微镜的情况下满足用户需求。因此，此应用程序的原因是：1：提供急需的仪器时间； 2替换不再可用作为核心显微镜的1210仪器。 自CBI于20年前开始运营以来，它已成为目前参与研究项目的医学研究社区的组成部分，该项目拥有250多个PHS资助的小组，以及在全国各地机构的调查人员的PHS支持项目中。随着PHS资助的用户群的不断扩大，缺乏质量的EM时间已成为该中心实用性的主要限制因素，这导致用户遇到极大的挫败感。