Imaging Core

成像核心

基本信息

批准号：
10631053
负责人：
SIMON C WATKINS
金额：
$ 21.76万
依托单位：
OHIO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-01-03 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10631053
关键词：
Acute Lung Injury Acute Respiratory Distress Syndrome Algorithmic Software Apoptosis Biological Biological Process Biology Cell Communication Cell Death Cell Survival Cells Collaborations Color Computer Assisted Confocal Microscopy Data Electron Microscopy Electrons Epithelial Cells Experimental Designs Faculty Fluorescence Microscopy Goals Image Image Analysis Imaging Techniques Immunobiology Immunosuppression In Vitro Individual Lasers Light Lipids Lung Measurement Messenger RNA Methods Microscopic Molecular Morphology Myeloid Cells Necrosis Optical Methods Optics Paper Pathology Pathway interactions Pattern Pneumonia Process Productivity Proteins Research Personnel Resolution Risk Factors Role Sampling Secondary to Services Structure Techniques Technology Tissue imaging Tissues Training Transmission Electron Microscopy animal imaging cell behavior cell fixing cell motility cell type cellular imaging chemokine combinatorial design experimental study in vivo innate immune function instrumentation light microscopy live cell imaging programs protein expression protein transport

项目摘要

Core C: Abstract The overarching theme of program is to dissect a new concept that in Acute Respiratory Distress Syndrome (ARDS), immunosuppression is a signature manifestation secondary to unique, combinatorial pathways that modulate epithelial and myeloid cell viability and innate immune function. We focus on pneumonia as a conceptual framework to study immunosuppression, as pneumonia is a common cause and risk factor for ARDS. The faculty within the Imaging Core have been collaborating continuously and productively with the PI’s of this program for 23 years in various aspects of immunobiology, lipid biology andairway/pulmonary biology resulting in 37 collaborative papers. The principal roles of the imaging core will be; careful analysis of cellular and molecular processes associated with apoptosis, necrosis, ferroptosis and other cell death mechanisms as needed, characterization and quantification of cell types within tissues, examination of protein expression during cell death, examination of cell-cell interactions in vitro, quantitative analysis of cellular migration in vivo and ex vivo individual cells within tissues, and quantitative measurement of the levels and patterns of expression of chemokines and cell-type markers various samples at all levels of resolution. These studies will employ the full array of current light, and potentially, electron microscopic methods including: single and multicolor fluorescence microscopy, laser confocal microscopy live cell imaging, transmission electron microscopy and computer-aided morphometric analyses . The Center for Biologic Imaging, in which this core service will be performed, is designed for the purpose of providing state of the art microscopic technologies to its users. It is equipped to perform a continuum of optical methods including all types of light and electron microscopy essential to this Program Project.

核心 C：摘要该计划的首要主题是剖析急性呼吸系统疾病中的一个新概念应激综合症（ARDS），免疫抑制是继发于调节上皮细胞和骨髓细胞活力和先天性的独特组合途径我们专注于肺炎作为研究的概念框架。免疫抑制，因为肺炎是 ARDS 的常见原因和危险因素。成像核心内部一直与 PI 进行持续且富有成效的合作该项目在免疫生物学、脂质生物学的各个方面已有 23 年的经验和气道/肺生物学产生了 37 篇合作论文。成像核心的主要作用是：仔细分析细胞和分子与细胞凋亡、坏死、铁死亡和其他细胞死亡机制相关的过程所需、组织内细胞类型的表征和定量、蛋白质检查细胞死亡期间的表达、体外细胞间相互作用的检查、定量分析组织内体内和离体单个细胞的细胞迁移，并定量趋化因子和细胞类型标记的表达水平和模式的测量这些研究将采用所有当前的分辨率水平的各种样本。光显微方法和潜在的电子显微方法，包括：单色和多色荧光显微镜、激光共聚焦显微镜、活细胞成像、透射电子显微镜和计算机辅助形态分析。生物成像中心。该核心服务将被执行，旨在提供它能够为用户提供连续的光学技术。方法，包括该计划项目所必需的所有类型的光学和电子显微镜。