Request for a Structured Illumination Super-Resolution Microscope

请求结构照明超分辨率显微镜

• 批准号: 8437557
• 负责人: SIMON C WATKINS
• 金额: $ 60万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8437557
• 关键词: Area; Biomedical Research; Communities; Core Facility; Devices; Dyes; Electrons; Funding; Growth; Housing; Image; Image Analysis; Institution; Laboratories; Light; Lighting; Medical; Medical Research; Methods; Microscope; Microscopy; Proteins; Research; Research Personnel; Research Project Grants; Resolution; Structure; System; United States National Institutes of Health; Universities; University of Pittsburgh Cancer Institute; fluorophore; improved; instrument; light microscopy; medical schools; operation; optical imaging

DESCRIPTION (provided by applicant): This request is for funds to purchase a Nikon super-resolution SIM system on a Ti inverted microscope. This instrument will be housed within the Center for Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of this core facility is to provide access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of Pittsburgh School of Medicine. Over the last few years super-resolution systems have become functionally useful in biomedical research, improving the resolution limit of light microscopy significantly. However, most of these approaches (STED, PALM/STORM) cannot be used with generically available fluorophores. High resolution methods (specifically Structured Illumination Microscopy (SIM)) which can be used with any currently available fluorophores, from organic dyes to genetically encoded proteins have only recently become commercially available in a "core suitable" format. Because of this and the fact that several of the investigators at our institution have real questions that truly benefit from the improved resolution available we are submitting this proposal. There are no other "core" super-resolution instruments in Pittsburgh. There is one CW STED instrument in a single investigators private laboratory, this cannot be used by other investigators. The requested microscope will be placed in the core laboratories of the CBI, and will be generally available to all NIH funded users of the Center. It is important to recognize that the CBI has two applications in this competition for microscopes. The other application, for a standard confocal will be placed in the new imaging core (a CBI satellite) in th University of Pittsburgh Cancer Institute. There is absolutely no redundancy between the two applications. Since the CBI commenced operations 20 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 200 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments and at neighboring institutions. The device requested here is a critical component of the continued growth of this institutional facility.

描述（由申请人提供）：此请求是要在Ti倒置显微镜上购买尼康超分辨率SIMS系统的资金。该乐器将安置在匹兹堡大学医学院的生物成像中心（CBI）中。该核心设施的任务是为匹兹堡大学医学院的所有研究小组提供全方位的光和电子光学，图像分析和形​​态学方法。 在过去的几年中，超分辨率系统在生物医学研究中已经有用，从而显着提高了光学显微镜的分辨率极限。但是，这些方法中的大多数（Sted，Palm/Storm）不能与一般可用的荧光团一起使用。高分辨率方法（特定的结构化照明显微镜（SIM）），可以与当前可用的荧光团一起使用，从有机染料到遗传编码的蛋白质，直到最近才以“核心适合”格式在市售。因此，这一事实是，我们机构中的几个调查人员都有真正的问题，这些问题确实从可用的改进决议中受益，我们提交了该提案。匹兹堡没有其他“核心”超分辨力工具。单个调查人员的私人实验室中有一种CW Sted工具，其他调查人员无法使用。所请求的显微镜将放置在CBI的核心实验室中，并且通常向中心的所有NIH资助的用户使用。 重要的是 认识到CBI在这项竞赛中具有两种应用，以进行显微镜。对于标准公约的另一个应用程序将放置在匹兹堡癌症研究所的新成像核心（CBI卫星）中。这两个应用程序之间绝对没有冗余。 自20年前CBI开始运营以来，它已成为目前参与医学研究项目不可或缺的一部分，该研究项目拥有200多个医疗领域内的PHS资助小组，以及在PHS的支持项目中，与其他部门和邻近机构的调查人员有关。这里要求的设备是该机构设施持续增长的关键组成部分。