Request for a Structured Illumination Super-Resolution Microscope

请求结构照明超分辨率显微镜

• 批准号: 8437557
• 负责人: SIMON C WATKINS
• 金额: $ 60万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8437557
• 关键词: Area; Biomedical Research; Communities; Core Facility; Devices; Dyes; Electrons; Funding; Growth; Housing; Image; Image Analysis; Institution; Laboratories; Light; Lighting; Medical; Medical Research; Methods; Microscope; Microscopy; Proteins; Research; Research Personnel; Research Project Grants; Resolution; Structure; System; United States National Institutes of Health; Universities; University of Pittsburgh Cancer Institute; fluorophore; improved; instrument; light microscopy; medical schools; operation; optical imaging

DESCRIPTION (provided by applicant): This request is for funds to purchase a Nikon super-resolution SIM system on a Ti inverted microscope. This instrument will be housed within the Center for Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of this core facility is to provide access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of Pittsburgh School of Medicine. Over the last few years super-resolution systems have become functionally useful in biomedical research, improving the resolution limit of light microscopy significantly. However, most of these approaches (STED, PALM/STORM) cannot be used with generically available fluorophores. High resolution methods (specifically Structured Illumination Microscopy (SIM)) which can be used with any currently available fluorophores, from organic dyes to genetically encoded proteins have only recently become commercially available in a "core suitable" format. Because of this and the fact that several of the investigators at our institution have real questions that truly benefit from the improved resolution available we are submitting this proposal. There are no other "core" super-resolution instruments in Pittsburgh. There is one CW STED instrument in a single investigators private laboratory, this cannot be used by other investigators. The requested microscope will be placed in the core laboratories of the CBI, and will be generally available to all NIH funded users of the Center. It is important to recognize that the CBI has two applications in this competition for microscopes. The other application, for a standard confocal will be placed in the new imaging core (a CBI satellite) in th University of Pittsburgh Cancer Institute. There is absolutely no redundancy between the two applications. Since the CBI commenced operations 20 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 200 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments and at neighboring institutions. The device requested here is a critical component of the continued growth of this institutional facility.

描述（由申请人提供）：此请求旨在为购买 Ti 倒置显微镜上的尼康超分辨率 SIM 系统提供资金。该仪器将安装在匹兹堡大学医学院的生物成像中心 (CBI) 内。该核心设施的任务是为匹兹堡大学医学院的所有研究小组提供全方位的光和电子光学、图像分析和形​​态测量方法。 在过去的几年中，超分辨率系统在生物医学研究中发挥了重要作用，显着提高了光学显微镜的分辨率极限。然而，大多数这些方法（STED、PALM/STORM）不能与通用荧光团一起使用。高分辨率方法（特别是结构照明显微镜（SIM））可以与任何当前可用的荧光团（从有机染料到基因编码的蛋白质）一起使用，直到最近才以“核心适合”的形式商业化。因此，我们机构的几位研究人员提出了真正受益于改进的解决方案的实际问题，我们提交了此提案。匹兹堡没有其他“核心”超分辨率仪器。单个研究者的私人实验室中有一台 CW STED 仪器，其他研究者不能使用该仪器。所需的显微镜将放置在 CBI 的核心实验室中，并将普遍提供给该中心的所有 NIH 资助的用户。 重要的是 认识到 CBI 在这场显微镜竞赛中有两个应用。标准共聚焦的另一个应用将被放置在匹兹堡大学癌症研究所的新成像核心（CBI 卫星）中。两个应用程序之间绝对没有冗余。 自 20 年前开始运作以来，CBI 已成为医学研究界不可分割的一部分，目前与医疗领域内 200 多个 PHS 资助的团体一起参与研究项目，以及与其他部门和研究人员一起参与 PHS 支持的项目。在邻近的机构。这里要求的设备是该机构设施持续发展的关键组成部分。