Request for a Nikon A1 confocal microscope

索取尼康 A1 共焦显微镜

• 批准号: 8825158
• 负责人: SIMON C WATKINS
• 金额: $ 35.79万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8825158
• 关键词: 10 year old; 14 year old; Area; Communities; Confocal Microscopy; Core Facility; Electrons; Frustration; Funding; Housing; Image; Image Analysis; Institution; Light; Medical; Medical Research; Methods; Microscope; Neck; Photons; Research; Research Personnel; Research Project Grants; Services; Time; United States National Institutes of Health; Universities; base; detector; instrument; medical schools; operation

 DESCRIPTION (provided by applicant): This request is for funds to purchase a basic single photon Nikon A1 confocal microscope with two GaSP detectors. This instrument will be housed within the Center for Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of this core facility is to provide access to a full range of light and electron optica, image analysis, and morphometric methods to all research groups within the University of Pittsburgh School of Medicine. Confocal microscopy is an essential service provided by the center, the current core use confocal microscopes within the facility are a 4 yr. old Nikon A1R, two Olympus FV1000's (8 and 10 years old) and a 14 year old Olympus fluoview 500, There is a single prime reason for this application: to provide critically needed instrument time to the NIH supported users of the Center for Biologic Imaging. Since the CBI commenced operations 22 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 300 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments and at neighboring institutions. The PHS funded user base within the institution continues to expand, and a lack of available confocal time has become a major limiting factor in the utility of the center, this leads to considerable frustration amongst users. The acquisition of a basic single photon confocal microscope will be a tremendous addition to the activity and functionality of the center and will help alleviate the current user bottle neck for single photon confocal microscopy.

 描述（通过应用程序证明）：该要求是基本的Singon Nikon A1共焦显微镜，并具有两个GASP检测器。匹兹堡医学院是由中心提供的，当前的核心核心核心核心核心套装PUS Fluoview 500，该应用程序有一个主要理由：为NIH支持的用户提供迫切需要的乐器中心成像。目前，医学研究界的一部分参与了300多个PHS资助的研究项目，以及其他部门和邻近的项目。共焦时间已成为中心效用的主要限制因素，导致用户的沮丧性造成了极大的挫败感。

项目成果

ISSLS prize in basic science 2021: a novel inducible system to regulate transgene expression of TIMP1.

2021年ISSLS基础科学奖：一种调节TIMP1转基因表达的新型诱导系统

• DOI: 10.1007/s00586-021-06728-0
• 发表时间: 2021-05
• 期刊: European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
• 作者: Han Y;Ouyang Z;Wawrose RA;Chen SR;Hallbaum M;Dong Q;Dando E;Tang Y;Wang B;Lee JY;Shaw JD;Kang JD;Sowa GA;Vo NN
• 通讯作者: Vo NN

Pathway crosstalk enables cells to interpret TGF-β duration.

• DOI: 10.1038/s41540-018-0060-5
• 发表时间: 2018
• 期刊: NPJ systems biology and applications
• 影响因子: 4
• 作者: Zhang J;Tian XJ;Chen YJ;Wang W;Watkins S;Xing J
• 通讯作者: Xing J

Arsenic disrupts extracellular vesicle-mediated signaling in regenerating myofibers.

砷会破坏肌纤维再生过程中细胞外囊泡介导的信号传导。

• DOI: 10.1093/toxsci/kfad075
• 发表时间: 2023
• 期刊: Toxicological sciences : an official journal of the Society of Toxicology
• 作者: Clemens,Zachary;Wang,Kai;Ambrosio,Fabrisia;Barchowsky,Aaron
• 通讯作者: Barchowsky,Aaron

Age-dependent changes in intervertebral disc cell mitochondria and bioenergetics.

• DOI: 10.22203/ecm.v036a13
• 发表时间: 2018-10-18
• 期刊: European cells & materials
• 影响因子: 3.1

MicroRNA-23a downregulates the expression of interferon regulatory factor-1 in hepatocellular carcinoma cells.

• DOI: 10.3892/or.2016.4864
• 发表时间: 2016-08
• 期刊: Oncology reports
• 影响因子: 4.2
• 作者: Yan Y;Liang Z;Du Q;Yang M;Geller DA
• 通讯作者: Geller DA