Request for live cell confocal microscope for BSL3 facility

请求为 BSL3 设施提供活细胞共聚焦显微镜

• 批准号: 7787740
• 负责人: SIMON C WATKINS
• 金额: $ 40.59万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7787740
• 关键词: Area; Cells; Communities; Confocal Microscopy; Containment; Devices; Electrons; Environment; Equipment; Funding; Grant; Growth; HIV; Housing; Image; Image Analysis; Institution; Laboratories; Life; Light; Medical; Medical Research; Methods; Microscope; Photons; Research; Research Personnel; Research Project Grants; Scanning; Services; Specimen; Universities; biosafety level 3 facility; cellular imaging; instrument; medical schools; operation; optical imaging; pathogen; time use

DESCRIPTION (provided by applicant): This request is for funds to purchase a Nikon Ti inverted microscope with widefield, DIC and confocal capabilities. This instrument will be housed within imaging core of the Regional Biocontainment Laboratory(RBL) at the University of Pittsburgh. This imaging core is a newly formed satellite facility of the Center for Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of the CBI is to provide access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of Pittsburgh School of Medicine. The building of an imaging core within the RBL is an essential facet of that mandate Confocal microscopy is an essential service provided by the center, and there are multiple point scanning single photon and one multiphoton microscope available to users, however these devices are within an open lab environment and can only be used with fixed or non-pathogenic specimens. In the last few years there has been a dramatic increase in the number of funded grants within our institution that need to image pathogenic specimens in real time using live cell imaging. This cannot be done at the moment and it is imperative we build a specialized imaging facility within a barrier facility. While some projects may be performed in a level 2+ facility (for example imaging HIV) there are an equal number of projects that demand a level 3 facility. Rather than build 2 satellites we have decided to build a single facility in level 3 containment space that will be used by all users who need access to live cell imaging at containment level 2+ or 3. Obtaining funds for a live cell confocal for this space is the focus of this application. It is important to recognize that the CBI has two applications in this competition for similar equipment. The other application, for a live cell confocal with TIRF capabilities will be placed in the 24 hr/day imaging core space, this microscope cannot be used by any of the researchers included in this proposal. Because of this there is absolutely no redundancy between the two applications. Since the CBI commenced operations 17 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 200 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments and at neighboring institutions. The device requested here is a critical component of the continued growth of this institutional facility, such that it can serve the needs of researchers who want to integrate live cell imaging of pathogens into their research.

描述（由申请人提供）：此请求是要资金购买具有广场，DIC和共聚焦功能的尼康Ti倒置显微镜。该乐器将安置在匹兹堡大学区域生物疗法实验室（RBL）的成像核心中。该成像核心是匹兹堡大学医学院生物成像中心（CBI）的新成立的卫星设施。 CBI的任务是为匹兹堡大学医学院的所有研究小组提供全方位的光和电子光学，图像分析和形​​态学方法的访问权限。 RBL内的成像核心的构建是该授权共聚焦显微镜的重要方面，是该中心提供的必不可少的服务，并且有多个点扫描单个光子和一个可供用户可供使用的多光子显微镜扫描，但是这些设备在开放式实验室环境中，只能与固定或非触发剂的样本一起使用。在过去的几年中，我们机构中资助的赠款数量急剧增加，需要使用活细胞成像实时对病原标本进行成像。目前不能做到这一点，我们必须在障碍设施中建立专门的成像设施。尽管某些项目可以在2级以上的设施（例如成像HIV）中执行，但需要相等数量的项目需要3级设施。我们决定在第3级围护空间中建立一个单个设施，而不是制造2个卫星，所有的用户都将使用在第2级或3级或3级访问Live Cell Imaging的用户使用。为此空间获得实时单元共享资金是该应用程序的重点。 重要的是要认识到，CBI在此竞赛中针对类似设备有两种应用。另一个应用程序，将在24小时/天成像核心空间中放置与TIRF功能的活细胞共焦，该提案中包括的任何研究人员都无法使用该显微镜。因此，这两个应用程序绝对没有冗余。 自17年前CBI开始运营以来，它已成为目前参与研究项目的医学研究社区的组成部分，其中有200多个PHS资助的小组，以及在PHS的支持项目中，在其他部门和邻近机构的调查人员中支持项目。这里要求的设备是该机构设施持续增长的关键组成部分，因此它可以满足希望将病原体的活细胞成像整合到其研究中的研究人员的需求。