Request for Zeiss 710 multiphoton 5Live Duoscan Microscope

索取蔡司 710 多光子 5Live Duoscan 显微镜

• 批准号: 7827587
• 负责人: SIMON C WATKINS
• 金额: $ 112.37万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-03 至 2012-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7827587
• 关键词: 6 year old; 9 year old; Area; Biological Models; Blood flow; Brain; Calcium; Communities; Core Facility; Country; Devices; Electrons; Fishes; Funding; Housing; Human; Image; Image Analysis; Institution; Light; Measurement; Medical; Medical Research; Methods; Microscope; Microscopy; Mus; Optics; Photons; Research; Research Personnel; Research Project Grants; Services; Slice; Speed; System; Universities; aged; instrument; medical schools; operation; optical imaging; 6 year old; 9 year old; Area; Biological Models; Blood flow; Brain; Calcium; Communities; Core Facility; Country; Devices; Electrons; Fishes; Funding; Housing; Human; Image; Image Analysis; Institution; Light; Measurement; Medical; Medical Research; Methods; Microscope; Microscopy; Mus; Optics; Photons; Research; Research Personnel; Research Project Grants; Services; Slice; Speed; System; Universities; aged; instrument; medical schools; operation; optical imaging

DESCRIPTION (provided by applicant): This request is for funds to purchase a multiphoton capable/single photon high speed confocal microscope. This instrument will be housed within the Center for Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of this core facility is to provide access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of Pittsburgh School of Medicine. Optical sectioning microscopy is an essential service provided by the center, the current core use confocal microscopes within the facility are a 3 yr old and a 2 yr old Olympus FV1000, a 9 year old Olympus fluoview 500, a similarly aged Leica TCS SL and a 6 year old Zeiss 510. We have also recently aquired an inverted single photon/multiphoton Olympus FV1000. The reasons for this application are to: 1: Provide access to multiphoton microscopy using an upright configuration for systems which cannot be studied using the inverted system currently within the CBI 2: Provide access to high speed confocal imaging for a variety projects ranging from imaging blood flow in developing model systems such as fish and mice, cillary beat in human airway cultures and calcium measurements in brain slices Since the CBI commenced operations 17 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 200 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments at neighboring institutions, and with collaborators around the country. The device requested here is an important and fundamental addition to the armamentarium of microscopies available to users of the CBI and it will have fundamental and significant impact on PHS funded research at the University of Pittsburgh and beyond.

描述（由申请人提供）：此请求是要资金购买多人/单个光子高速共聚焦显微镜。该乐器将安置在匹兹堡大学医学院的生物成像中心（CBI）中。该核心设施的任务是为匹兹堡大学医学院的所有研究小组提供全方位的光和电子光学，图像分析和形​​态学方法。 光学切片显微镜是该中心提供的必不可少的服务，该设施内的当前核心使用共聚焦显微镜为3岁，一个2岁的Olympus FV1000，一个9岁的Olympus Fluoview 500，同样年迈的Leica TCS SL和6岁的Zeiss 510。 The reasons for this application are to: 1: Provide access to multiphoton microscopy using an upright configuration for systems which cannot be studied using the inverted system currently within the CBI 2: Provide access to high speed confocal imaging for a variety projects ranging from imaging blood flow in developing model systems such as fish and mice, cillary beat in human airway cultures and calcium measurements in brain slices Since the CBI commenced operations 17 years ago, it has become an目前，医学研究界不可或缺的一部分参与研究项目，其中有200多个PHS资助的小组，以及在邻近机构其他部门的调查人员以及全国各地的合作者的PHS支持项目中。这里要求的设备是可用于CBI用户可用的显微镜​​的重要且根本的补充，它将对匹兹堡大学及其他地区的PHS资助研究产生根本和重大影响。